The effects ofBrassica junceaL. leaf extract on obesity and lipid profiles of rats fed a high-fat/high-cholesterol diet

Abstract

BACKGROUND/OBJECTIVESObesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats.MATERIALS/METHODSMale Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively.RESULTSThe HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol 7α-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor α. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE.CONCLUSIONSSupplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.

Eruca sativa Mill. seed extract promotes anti‐obesity and hypoglycemic effects in mice fed with a high‐fat diet

“…These results are in accordance with the literature published on other Brassicaceae vegetables; indeed, many preclinical and clinical reports demonstrated their antioxidant activity (Bahadoran et al, 2011), anti-obesity and hypo-lipidemic effects in rats fed with HF diet (Lee, Kim, & Lee, 2018). For instance, 4-week supplementation with Brassica rapa L. (var.…”

Effect of concomitant therapy of anti-diabetics and hypolipidemics on biochemical and histological parameters in animal models

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Phytochemistry and biological activity of mustard (Brassica juncea): a review

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