The Effects of Saccharomyces boulardii on Bacterial Translocation in Rats with Obstructive Jaundice

Abstract

INTRODUCTION The aim of this study was to investigate the effect of Saccharomyces boulardii treatment on preventing bacterial translocation in an obstructive jaundice animal model. MATERIALS AND METHODS Sixty adult rats were divided into five groups: group 1 -the sham-operated group; group 2 -the common bile duct ligation group; group 3 -the S. boulardii group; group 4 -the ampicillin-sulbaktam group; and group 5 -the S. boulardii plus ampicillin-sulbaktam group. The saline, antibiotics and S. boulardii were given, respectively, for a 7-day period as a single dose per day via temporary orogastric intubation. Seven days following the obstructive jaundice, the animal had laparatomy under sterile conditions. Segments of ileum were removed for histopathological examination. Blood, liver, spleen and mesenteric lymph nodes were taken for microbiological culture. RESULTS Bacterial translocation rates were 0% in the sham-operated group, 83% in group 2, 42% in group 3, 42% in group 4 and 33% in group 5. Bacterial translocation significantly increased in group 2 compared to groups 3, 4 and 5 (P = 0.001). The bacterial counts (CFU/g) of group 2 were significantly higher than those of groups 3, 4 and 5 (P = 0.001). Histopathological examination of ileum specimens revealed a significant decrease in the heights of villi in groups 2-5 compared to the sham-operated group (P = 0.001). The mean villus height in groups 3 and 5 was significantly higher than that of group 4 (P = 0.001).CONCLUSIONS S. boulardii was found to be effective in the successful control of translocation and improvement of intestinal barrier function.

Evaluation of the effects of Aegle marmelos and Punica granatum in an experimental model of gastrointestinal barrier dysfunction

“…14 Hence various researchers have assessed villous morphology including villous heights to evaluate the effect of the test drugs in the model of common bile duct ligation. 15,16 In our study, A. marmelos and P. granatum significantly prevented the blunting of villi and maintained the villous heights and the higher doses were comparable to glutamine. The exact mechanism by which A. marmelos and P. granatum prevented the damage to the intestinal mucosa is not known.…”

Effects on Bacterial Translocation of High-Fat Enteral Nutrition in Bile Duct Ligated Rats

“…Although the BT in bile duct ligated models was investigated before (15)(16)(17)(18)(19)(20)(21)(22), in many of them (15)(16)(17)(19)(20)(21)(22)) the effects of different materials on BT, except food with high-fa was studiedt. In previous studies, the effects of high-fat enteral nutrition on BT were studied by Luyer et al (10,11,18).…”

“…Up to now, some studies which investigate the BT in obstructive jaundice models have been carried out (15)(16)(17)(18)(19)(20)(21)(22). In some of them, different materials were given to the models in whom bile duct was ligated to observe their effects on BT (16)(17)(18)(19)(20)(21)(22).…”

“…In some of them, different materials were given to the models in whom bile duct was ligated to observe their effects on BT (16)(17)(18)(19)(20)(21)(22). Erbil et al (16) have studied the effects of deoxycholate, lactulose and glutamine on BT included by obstructive jaundice.…”

“…Luyer et al (18) have investigated the levels of plasma endotoxin and TNF-α on the models which have bile duct ligation and fed by high-fat nutrition during seven days after a hemorrhagic shock procedure. Geyik et al (21) have studied the effects of an yeast on BT in the models with obstructive jaundice. Gencay et al (22) have reported the effect of honey on BT in obstructive jaundice.…”