The Effects of In-Ovo Injection of Propolis on Egg Hatchability and Starter Live Performance of Japanese Quails

Abstract

KeywordsIn-ovo feeding, propolis, hatching traits, quail.Submitted: August/2016 Approved: September/2016 ABSTRACT The purpose of this study was to determine the effects of in-ovo injection of a propolis water extract on hatchability, embryonic mortality, starter live performance, and livability of Japanese quails. In total, 500 fresh hatching eggs were randomly distributed into five treatment groups of 100 eggs per treatment with four replicates of 25 eggs each. On day 14 of incubation, eggs from group 1 were not injected (control), group 2 was injected with distilled water (water), group 3 was injected with 1% propolis water extract (1% propolis), group 4 was injected with 2% propolis water extract (2% propolis), and group 5 was injected with 3% propolis water extract (3% propolis). A completely randomized design was applied, and data were analyzed using the least-square methodology. Hatchability and embryonic mortality in the 2% propolis and 3% propolis treatment groups were significantly lower compared with the control group, but no significant differences were observed between the 1% propolis and control groups. There were no significant bodyweight gain, feed intake, feed conversion ratio, or livability differences among treatments. The results of this study demonstrated that in-ovo injection of propolis water extract, especially at doses of 2% and 3% propolis, had negative effects on hatchability and embryonic mortality, but 1% propolis had no detrimental effects on hatchability or embryonic mortality. In all treatment groups, propolis did not negatively affect body weight gain, feed intake, feed conversion ratio, or livability.

The immune adjuvant effect of Astragalus polysaccharide on in ovo injection of Newcastle disease vaccine

“…Our study was contrary to the findings of Aygun (2016), which demonstrated that plant extracts of thyme and savory could improve immunity, but not the embryonic hatchability. Our results revealed both APS and live ND vaccine adjuvanted with APS appeared to significantly enhance hatchability; however, the live ND vaccine only did not change the hatchability when compared to Lymphocytes proliferation rate is an indicator of the status of cellular immunity (Zhao, Hu, Wang, Liu, & Guo, 2013), has been widely used to evaluate immunogenetic capability of an adjuvant.…”

“…Adjuvants aiming to strengthen immune responses have been extensively exploited in vaccine schemes (Coffman, Sher, & Seder, 2010). For example, certain polysaccharides extracted from Chinese medicines used as vaccine adjuvants could effectively enhance vaccination efficacies (Aygun, 2016; Saki & Salary, 2015). In this study, we evaluated the effects of Astragalus polysaccharide (APS) as a ND vaccine adjuvant on the humoral and cellular immune responses in chicks through in ovo embryonic vaccination.…”

Impact of hen treatment with bee pollen and thermal manipulation during early egg incubation period on the hatchability and embryonic development of chicks

“…The increased DAP and EM percentages in the control group (untreated) may be due to increasing water loss percentage from the eggs leading to high DAP as well as EM because of dehydration. These results disagree with the findings of Aygun (2016) who found that the hatchability of fertile eggs and EM for eggs treated with distilled water and injected with 1, 2 and 3% propolis water extract were not affected. Also, the findings of Abuoghaba (2017) showed that the DAP, EM were not affected by ascorbic acid treatment or the interaction between incubation temperature and ascorbic acid treatment.…”

“…This could be attributed to the role of BP in the reduction of adverse effects of high incubation temperature. These results agree with Aygun (2016) who stated that the chick body weight at hatching was not affected by the in ovo injection of 1, 2 and 3% propolis during the incubation period.…”

In ovo injection of flavanone on bone quality characteristics, biochemical parameters and antioxidant enzyme status of blood in daily chicks

“…The defence mechanisms like enzymes and natural antioxidants (Surai, ) protect the developing chick. The IOI of nutrients with antioxidant roles may help to overcome any limitation of inadequate egg nutrition and improve antioxidative status (Aygun, ).…”