The effects of in ovo administered bisphenol A on tibial growth plate histology in chicken

Abstract

Objectives The aim of this study was to determine of the effects of in ovo administered BPA on embryonic development of the tibial growth plate using histological methods in chickens. Methods Three hundred and ten fertile eggs of Isa Brown laying parent stock were divided into five groups as untreated control, vehicle‐injected control, 50, 100, and 250 μg/egg BPA. At the 13th, 18th, and 21st days of incubation, eggs were randomly opened from each group until 10 live embryos were obtained. Embryos were weighed and crown‐rump length was measured. Tibial tissue samples were taken from embryos. Tibia weight, relative tibia weight and tibia length were determined. Tissue samples were fixed in 10% buffered formalin solution. Sections were stained with Safranin O staining methods and zones in the growth plate were measured. Also, proliferating cell nuclear antigen (PCNA) was stained immunohistochemically. Results The mortality in the BPA treated groups was higher than untreated control group. The results have revealed that mean relative embryo weights, crown‐rump length, mean tibia weight, relative tibia weight, and tibia length of BPA treated groups were significantly lower when compared to the untreated control and vehicle‐injected control groups. Also, proliferative zone get significantly narrowed, whereas the transitional and hypertrophic zone thickened and PCNA positive chondrocytes increased in growth plate of BPA treated groups. Conclusion These results have suggested that developmental exposure to BPA adversely affected development of the tibial growth plate.

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“…Dietary MLF for breeder hens significantly increased the level of p-mTOR/mTOR in breast muscle of chicks at 1 d after hatching, indicating that the up-regulated expression of p-mTOR was an important effector molecule to promote the development of offspring. A number of studies have considered the relative breast muscle weight and relative tibial length during the incubation as important parameters for embryonic development ( Akil and Zakaria, 2015 ; Öznurlu et al., 2021 ; Piestun et al., 2015 ). And BMP is involved in osteoblast differentiation and bone formation regulation ( Gomez-Puerto et al., 2019 ).…”

Investigation of the Effects of Monosodium Glutamate on the Embryonic Development of the Eye in Chickens

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