The Effects of Induced Physical and Oxidative Stress on the Cortisol Levels of Female Wistar Rat

Shugaba, AI; Hambolu, JO; Ojo, SA

doi:10.4314/jmt.v12i2.69321

Cited by 7 publications

(6 citation statements)

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“…Data here are consistent with these findings, showing an increase in unhealthy follicles, and a reduction in total follicle number, following treatment with Cisplatin [27,46]. According to Perez [47], mice exposed to chemotherapeutic agents displayed apoptosis of granulosa cells in primordial follicles.…”

Section: The Morphological Effectssupporting

confidence: 79%

Cisplatin-Induced Ovarian Cytotoxicity and the Modulating Role of Aqueous Zest Extract of Citrus limonium (AZECL) in Rat Models

Gg¹,

Oa²,

Uk³

et al. 2017

J Tradi Med Clin Natur

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Cisplatin is a prominent member of the effective broad-spectrum antitumor drugs. However, its clinical usage is restricted due to some adverse side effects, such as testiculototoxicity, hepatotoxicity and nephrotoxicity. The aim of this study is to evaluate the effect of Aqueous Zest Extract of Citrus limonium (AZECL) on the ovary of female Wistar rat treated with Cisplatin. Twenty adult female Wistar rats were divided into four groups (A-D) containing five rats each. Group A rats served as negative control and were treated orally with 2.5 ml/kg body weight of normal saline, group B rats served as positive control group and were treated intraperitoneally with a single dose of 10 mg/kg body weight of Cisplatin, group C rats were treated orally with 50 mg/kg body weight of AZECL and group D rats were treated intraperitoneally with a single dose of 10 mg/kg body weight of Cisplatin and two weeks later treated orally with 50 mg/kg body weight of AZECL. Result showed a significant (p<0.01) decrease in primary follicles, secondary follicles, graafian follicles and a significant (p<0.01) increase in atretic follicles, PAS positive reaction and reduction in the total carbohydrate contents of the stromal cells in the positive control group. Also there was a significant (p<0.05) decrease in the activity level of FSH, LH and a significant (p<0.05) increase in Malondialdehyde when compared to rats in group A and C. The group post-treated with the extract had remarkable normalization of the histo-morphometric, histochemical and biochemical parameters when compared to the positive control group. Aqueous zests extract of C. limonium has a curative effect on cisplatin-induced cytotoxicity on the ovary.

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Section: The Morphological Effectssupporting

confidence: 79%

Cisplatin-Induced Ovarian Cytotoxicity and the Modulating Role of Aqueous Zest Extract of Citrus limonium (AZECL) in Rat Models

Gg¹,

Oa²,

Uk³

et al. 2017

J Tradi Med Clin Natur

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show abstract

“…Serum total proteins (Armstrong et al, 1964) and albumin (Doumas et al, 1971) were determined, while globulin was calculated by subtracting the values of albumin from corresponding values of total proteins for each sample. Total Lipids (Postma and SWroes, 1968), total cholesterol (Kostner et al, 1979), AST and ALT (Reitman and Frankel, 1957), alkaline phosphatase (Tietz , 1976) and cortisol (Shugaba et al, 2010), and progesterone (Nulsen et al, 1992) were determined. All serum biochemical parameters were done using commercial kits (Diagnostic System Laboratories, Inc., USA).…”

Section: Blood Samplesmentioning

confidence: 99%

“…Cortisol and progesterone are affected rapidly by heat loads. There are relationships between the high level of cortisol and both ovum and ovulation development (Shugaba et al, 2010). Moreover, alkaline phosphatase (ALP), ALT and AST are important biochemical parameters (Chaurasia et al, 2016).…”

Section: Hormonal Profile and Enzyme Activitymentioning

confidence: 99%

Effect of Toxin Binder Supplemented to Friesian Cows Rations on Stress States and Their Reproductive Performance Under Summer Climatic Conditions in Delta Region تأثیر مضادات السموم الفطریة المضافة لعلائق الأبقار الفریزیان على حالة الاجهاد الحرارى و الأداء التناسلى لها تحت الظروف المناخیة الصیفیة لأقلیم الدلتا

Mehany

Hegazy

2020

Journal of Animal and Poultry Production

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Twenty Holstein cows with an average of 54017.59 kg LBW and 2-5 parities were used from beginning dry period to 120-days postpartum to study the effect of dietary bentonite and/or zeolite supplementation on reproductive performance under heat stress of Delta region. The experimental cows were distributed into four homogenous groups (5 cows each). All cows were fed a basal ration (BR) according to their production stage and body weight. The first group (G1) was fed (BR) only without any supplementation (control), otherwise groups G2, G3 and G4 were fed control diet plus 2 % bentonite, 1% bentonite and 1% zeolite or 2% zeolite of DM intake, respectively. The obtained results showed that dry matter intake, total proteins, albumin, Albumin/Globulin ratio, progesterone and birth weight of newborns were higher in treatment than in control groups. Rectal temperature, respiratory rate, the interval to 1 st ovulation, oestrus and service, days open, and the number of services per conception were lower (P<0.05), while conception rate was higher (P<0.05) in treatment than un-supplemented groups. In conclusion, bentonite supplementation as an antitoxin to ration of lactating Holstein cows at a level of 2% of DM intake under summer months in Delta region had a positive impact on feed intake, health status, and reproductive parameters.

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“…It is also possible that the membranes of these lymphocytes were oxidized as the rats were subjected to Ikhueniro waste dumpsite leachate; as a high concentration of lymphocytes in the blood is suggestive of a high degree of infection after trauma leading to high antibody production (Abbas and Lichtman, 2003). Increased granulocyte levels suggest high cellular damage/inflammation and depressed immunity; while increased monocyte count might be a sign of a chronic infection, an autoimmune disorder, or a blood disorder (Shugaba et al, 2012). One of the major functions of lymphocytes is their response to antigens (foreign bodies) by forming antibodies that circulate in the blood or the development of cellular immunity (Frandson, 2003).…”

Section: Ibezute a C; Makun O J; Tawari-fufeyin Pmentioning

confidence: 99%

Impact Of Ikhueniro Dumpsite Leachate On Blood Profile And Blood Film Of Wistar Rats

Ibezute¹,

Makun²,

Tawari-Fufeyin³

2023

jasem

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The challenge of environmental toxicity and the associated unrelenting efforts of man to abate or eradicate its hazards are lifelong efforts. The present study investigated the impact of Ikhueniro dumpsite leachate in Benin City, Nigeria on the hematological blood profile and blood film of Wistar rats. A total of 30 Wistar rats were acclimatized for two weeks and randomly distributed into three groups A to C; group A served as control, while groups B and C were orally administered Leachate only and Leachate + Abatement (Dialium guineenses aqueous leaf extract), respectively, once every 48 hours for 30 days. After the exposure period, the surviving rats in group A and half of the rats in group B and C were examined and sacrificed. The experiment continued with the remaining rats in groups B and C. Group B was given only clean water and C only abatement following ealier stated schedule, after which the surviving rats were sacrificed and samples collected. Hematological analysis showed that leachate administration in Wistar rats caused an increase in white blood cells (27.65%), platelet count (13.35%), and platelet crit (45.45%); with a decrease in red blood cell count (7.52%), hemoglobin (5.40%) and hematocrit (6.12%) of Wistar rats when compared to the control. Geimsa-stained blood film revealed the presence of polymorphs and basophils in the blood film of rats administered dumpsite leachate. Nevertheless, when leachate administration was stopped in the group of rats where it was previously administered, there was a slight improvement in some blood hematological indices. However, the administration of Dialiumguineense leaf extracts better-improved blood hematological indices. The findings of this study indicate that Ikhueniro dumpsite leachate negatively impacted blood hematological indices in Wistar rats. Dumpsite leachate should be handled and treated carefully to avoid potential health hazards in animals.

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The Effects of Induced Physical and Oxidative Stress on the Cortisol Levels of Female Wistar Rat

Cited by 7 publications

References 0 publications

Cisplatin-Induced Ovarian Cytotoxicity and the Modulating Role of Aqueous Zest Extract of Citrus limonium (AZECL) in Rat Models

Cisplatin-Induced Ovarian Cytotoxicity and the Modulating Role of Aqueous Zest Extract of Citrus limonium (AZECL) in Rat Models

Impact Of Ikhueniro Dumpsite Leachate On Blood Profile And Blood Film Of Wistar Rats

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