Executive SummarySpecies interactions research was initiated in 1989 to investigate ecological interactions among fish in response to proposed supplementation of salmon and steelhead in the upper Yakima River basin. This is the sixth of a series of progress reports that address species interactions research and pre-supplementation monitoring of fishes in the Yakima River basin. Data have been collected prior to supplementation to characterize the ecology and demographics of non-target taxa (NTT) and target taxon, predict the potential interactions that may occur as a result of supplementation, and develop methods to monitor interactions and supplementation success. Major topics of this report are associated with the ecology and life history of NTT, interactions experimentation, methods for sampling, risk assessment, and risk minimization. This report is organized into 14 chapters, with a general introduction preceding the first chapter. This annual report summarizes data collected primarily by the Washington Department of Fish and Wildlife (WDFW) between January 1, 1995 and December 31, 1997 in the Yakima basin, however these data were compared to data from previous years to identify preliminary trends and patterns. Major preliminary findings from each of the chapters included in this report are described below.o Objectives for highly valued non-target taxa (NTT) were described as acceptable impacts to their current status that could be attributed to spring chinook salmon supplementation. Impacts to a NTT's distribution, abundance, or size structure in excess of the acceptable impact would indicate a failure to achieve an objective. The following objectives for NTT are recommended: accept no impact -bull trout, westslope cutthroat trout, Pacific lamprey; accept a maximum of 5% impact -Marion Drain fall chinook salmon, upper Yakima steelhead, mountain sucker, leopard dace, sand roller; accept a maximum of 10% impact -resident rainbow trout in the mainstem Yakima River, Naches steelhead, Satus steelhead, Toppenish steelhead, Naches spring chinook salmon, American River spring chinook salmon; accept a maximum of 40% impactmountain whitefish, resident rainbow trout in tributaries; and accept impacts up to a maximum that maintains all native species at sustainable levels. These objectives reflect the goal of having all native species at sustainable levels including populations robust enough to support harvest and recreation.o Certain species or guilds within an ecosystem may interact strongly with spring chinook salmon resulting in failure to achieve desired numerical goals of spring chinook salmon through supplementation. Monitoring the influence of strong interactor taxa relative to spring chinook salmon will aid in interpreting factors that may influence YFP success. Recommended objectives for strong interactors are of two types: 1) identify and reduce impacts of unnaturally high predatory, pathogenic, and competitory interactions that limit spring chinook salmon productivity, and 2) identify, conserve and enhance...