The effects of intrafollicular injections of plasmin and E-aminocaproic acid on the ovulation in the ewe

Smokovitis, A.; Kouimtzis, S.; Koutsouris, C.; Kokolis, N.; Kouskoura, Th

doi:10.1016/0268-9499(89)90051-9

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…Plasminogen activator activity (PAA) has been also detected into various fluids and cell types of the female reproductive system, including follicular fluid (Smokovitis et al. , ), oviductal fluid (Gabler et al. ), cumulus‐oocyte complexes (Liu and Hsueh ; Yamada et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Krania

Dovolou

Rekkas

et al. 2014

Reprod Domestic Animals

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Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue-type plasminogen activator (t-PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus-oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t-PA and/or its inhibitor epsilon-aminocaproic acid (control, t-PA, t-PA+ε-ACA, ε-ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t-PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε-ACA. PAA in the treated group was significantly reduced by ε-ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t-PA-modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t-PA. In conclusion, it appears that excessive t-PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.

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Section: Introductionmentioning

confidence: 99%

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Krania

Dovolou

Rekkas

et al. 2014

Reprod Domestic Animals

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“…Plasmin is a trypsin‐like protease which can cleave a wide spectrum of substrates (Dano et al ., 1985). There is an accumulation of evidence that PA/plasmin can exert directly and/or indirectly extracellular matrix degradation (Smokovitis et al ., 1988, 1989). Besides the extracellular proteolytic effect of plasmin, this enzyme can degrade even membrane proteins (Christman et al ., 1977).…”

Section: Introductionmentioning

confidence: 99%

Induction of the acrosome reaction in bull spermatozoa with plasmin

2003

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Proteolytic enzymes appear to have an essential role in multiple phases of mammalian fertilization. Plasmin, the active enzyme of the plasminogen activation system that stimulates fibrinolysis and proteolysis has a less well-documented role in reproduction. The current study was conducted to investigate the effect of the active protease, plasmin, on the ability of bovine sperm to undergo the acrosome reaction. Aliquots of freshly ejaculated bull sperm were incubated in capacitating conditions with 10 microg ml-1 of heparin for 4 h. Every 2 h an aliquot of spermatozoa was exposed to lysophosphatidylcholine (100 microg ml-1) or 0, 0.1, 1, 10 and 100 mU of plasmin to induce the acrosome reaction in capacitated spermatozoa. Plasmin increased the percentage of live acrosome reacted sperm after 4 h of incubation in the capacitation medium. Viability was not affected by any of the treatments. This study provides new information on bovine acrosome reaction during in vitro incubation with plasmin and indicates that this protease may participate in the proteolytic events that accompany fertilization.

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“…The membrane localization of PAs in spermatozoa indicates that these proteolytic enzymes could participate in the process of fertilization. Early studies have shown that PA/plasmin can exert a direct and/or an indirect effect on extracellular matrix degradation (Danø et al ., 1985; Smokovitis et al ., 1988, 1989; Liotta et al ., 1991). Plasmin, which has a broad substrate specificity, can even attack membrane proteins (Christman et al ., 1977; Saksela & Rifkin, 1988).…”

Section: Discussionmentioning

confidence: 99%

Gossypol-induced inhibition of plasminogen activator activity in human and ovine acrosomal extract

et al. 1999

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The effect of gossypol--a polycyclic compound isolated from cotton seeds--on the plasminogen activator activity of man and ram acrosomal extracts was explored in vitro. The action of gossypol on the plasminogen activator activity was investigated by a spectrophotometric method using the chromogenic substrate S-2251. Gossypol, a known antispermatogenic agent, was found to effectively inhibit human and ovine acrosomal plasminogen activator activity. The inhibition was dose-dependent. Plasminogen activator activity from man and ram extracts was completely inhibited by 350 mumol l-1 and 300 mumol l-1 of gossypol, respectively. In additional experiments, low, non-spermicidal concentrations of gossypol (2.5-40 mumol l-1) were found to significantly inhibit plasmin activity in a dose-dependent manner. The results suggest that inhibition of both acrosomal plasminogen activator and plasmin activity is a possible mechanism by which gossypol exerts its antifertility effect, since the plasminogen activator/plasmin system plays a role in the whole process of ovum fertilization.

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The effects of intrafollicular injections of plasmin and E-aminocaproic acid on the ovulation in the ewe

Cited by 11 publications

References 33 publications

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Induction of the acrosome reaction in bull spermatozoa with plasmin

Gossypol-induced inhibition of plasminogen activator activity in human and ovine acrosomal extract

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