The effects of levetiracetam on neural tube development in the early stage of chick embryos

Güvenç, Yahya; Dalgıç, Ali; Billur, Deniz; Karaoğlu, Derya; Aydin, Sevim; Dağlıoğlu, Ergün; Özdöl, Çağatay; Nacar, Osman Arikan; Yıldırım, Ali Erdem; Belen, Deniz

doi:10.5137/1019-5149.jtn.7471-13.0

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…Many studies have been done on chicken embryos to investigate the etiology of NTD. They evaluated the effects of environmental factors and drugs (Cetinkal et al, 2010;Dalgic et al, 2009;Guvenc et al, 2013;Simsek et al, 2012;Temiz et al, 2009;Whitsel et al, 2002). In this study, it was observed that the width of the neural tube was altered with the Riparin III concentrations evaluated.…”

Section: Discussionmentioning

confidence: 70%

“…The period between the appearance of neural plaque to closure of the palate, that is, the period between the 18th and 60th day of pregnancy, is the period when the possibility of congenital anomaly is highest. These anomalies originate from an insufficiency in neural tube formation (Guvenc et al, 2013(Guvenc et al, , 2016 or re-opening after formation of the neural tube (Gardner, 1960). Environmental factors that would influence fetus development may cause congenital anomalies during this period.…”

Section: Discussionmentioning

confidence: 99%

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Riparin III effect on the development of neural tube embryos of Gallus gallus

Lima¹,

Filho²,

Gutierrez³

et al. 2017

Afr. J. Pharm. Pharmacol.

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The aim of this study is to demonstrate the teratogenicity of Riparin III in embryos of Gallus gallus (domestic) at an early stage of development. This study is unprecedented in the literature. Chicken eggs were used to investigate the morphometry. All the groups were incubated at 37.2 ± 0.1°C and 60 ± 5% relative humidity for 24 h. The embryonic disc was identified and the eggs of the control group were administered 0.1 ml of saline tamponade (PBS, pH 7.0) and those of the other group were administered Riparin III in 25, 50 and 100 μg/ml doses. The eggs were closed with sterile adhesive strips and incubation was continued for another 24 h. All the eggs were then reopened and the embryos dissected from embryonic membranes and evaluated histopathologically with haematoxylin eosin dye for 15 s and washed with distilled water. An embryological development within twelve stages as classified by Hamburger and Hamilton (1951) was obtained. When compared with the control group, the width of the neural tube of the embryos was modified with all Riparin III concentrations. Longer somites resulted from the 50 and 100 μg/ml Riparina III concentrations. The function of the structures was preserved. This drug can potentially be used in pregnant women as an anxiolytic and for treating depression.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Riparin III effect on the development of neural tube embryos of Gallus gallus

Lima¹,

Filho²,

Gutierrez³

et al. 2017

Afr. J. Pharm. Pharmacol.

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“…Also, intraperitoneal daily injection of 1200 mg/kg/day LEV during the period of organogenesis to pregnant mice increased the overall frequency of fetal skeletal abnormalities (Isoherranen et al, 2003). Similarly, fetal skeletal abnormalities were increased in rats and rabbits treated with LEV throughout gestation and/or during organogenesis On the contrary, according to Guvenc et al (2013) study, developmental anomalies were not seen at low doses of LEV in an early chick embryo model, and this finding may indicate that there were no developmental anomalies during pregnancy.…”

Section: Discussionmentioning

confidence: 98%

A Comparative Study of the Teratogenic Effects of Antiepileptic Drugs: Lamotrigine and Levetiracetam on Adult Albino Rats

Elgndy

Hagag

Kholy

et al. 2019

The Egyptian Journal of Forensic Sciences and Applied Toxicolog

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Background: Lamotrigine (LTG) and levetiracetam (LEV) are widely and increasingly prescribed second generation antiepileptic drugs for women with epilepsy during childbearing age. However, data pertaining to their congenital malformations are lacking. Aim: This study aimed to investigate and compare the teratogenic effects of LTG and LEV on normal adult female albino rats and their fetuses in order to find out the least teratogenic one of them. Materials and methods: Ninety-six adult albino rats (64 virgin females and 32 males) of almost the same range of weight and age were used. Pregnant rats were divided into 4 groups. The 1 st group (control; received 1 ml of distilled water) and the 2 nd group (vehicle; received 1 ml of 2% Arabic gum), while the 3 rd (LTG-treated) and 4 th (LEV-treated) groups received 50 mg/kg/b.wt. of LTG and 310 mg/kg/b.wt. of LEV, respectively. All substances were administered as single oral dose starting from gestational day (GD) 7 to 15. On GD 20, all dams were weighted then sacrificed and subjected to Cesarean section. The total numbers of ovarian corpora lutea and the status of all implantation sites (the total numbers of resorption sites, live and dead fetuses, and the total implantations) were counted. The gravid uteri were subsequently removed, weighted, and fetuses were delivered. The fetuses' numbers, weights, different body lengths, gross abnormalities, and skeletal malformations were recorded. Results: On GD 20, maternal weight gains and gravid uteri weights were statistically significantly lower in both treated groups than control and these differences were more significant in LTG than LEV. The percentages of pre-implantation and post-implantation losses in LTG and LEV exhibited statistically significant increase and non-significant differences compared with control, respectively, and the latter was more pronounced in LTG than LEV. As regard the numbers, weights, crown-rump lengths, biparietal diameters and head lengths of the fetuses, both treated groups showed significant differences in comparison to the control with higher abnormalities in LTG-treated than LEV-treated groups. Fetuses from treated groups revealed various gross abnormalities in the internal organs. Fetuses' skeletons examination revealed bone defects in both treated groups. Skull, vertebrae, and hind limb skeletal malformations were more prevalent in LTG-treated group, while sternum was more affected in LEV-treated group. Conclusion: Both antiepileptic drugs were teratogenic in variable degrees; however, LEV appears to be less teratogenic than LTG.

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“…Previously, substances that were thought to be toxic for the neural tube (NT) during pregnancy were investigated using the chicken embryo model (Emon, Orakdogen, Uslu, & Somay, 2015;Mete et al, 2016). In these studies, the effect of teratogenic substances was investigated on NT closure (Ertekin et al, 2019;Guvenc et al, 2013Guvenc et al, , 2016. In addition, the effects of factors other than teratogenic agents have been investigated in recent chicken embryo studies (Kantarcioglu et al, 2018).…”

mentioning

confidence: 99%

Impact of Bisphenol A on neural tube development in 48‐hr chicken embryos

Atay

Ertekin

Bozkurt

et al. 2020

Birth Defects Research

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Objectives: Bisphenol A (BPA) is one of the most heavily produced chemicals in the world. BPA is involved in the production of many substances such as cosmetics, various foodstuffs, toys, personal care products, detergents and plastic bottles all that are frequently used in daily life. Depending on BPA exposure, sexual maturation and reproductive function, and bone and brain development are adversely affected. The aim of this study is to investigate the possible effects of BPA on the development of the nervous system and neural tube in 48-hr chicken embryos. Methods: Thirty specific pathogen-free (SPF) fertilized eggs were used in the study. SPF eggs were placed in the incubator and divided into three groups at 28 hr of incubation; control, BPA 1 and BPA 2 (10 eggs in each group). At this stage of incubation, two different doses of BPA were injected subblastodermically with the Hamilton microinjector. At the end of 48 hr of incubation, all eggs were opened and embryos were dissected and separated from the embryonic membrane. All embryos were evaluated morphologically and histopathologically. Results: As the BPA dose increased, delays in the development of the nervous system and midline closure increased in the early period of chicken embryos. Depending on the dose, it was found that the embryo's crown-rump length and somite number decreased (p < .05). Conclusion: It was determined that BPA application on early chicken embryos adversely affected neural tube development. It was also found to delay midline closure.

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The effects of levetiracetam on neural tube development in the early stage of chick embryos

Cited by 10 publications

References 14 publications

Riparin III effect on the development of neural tube embryos of Gallus gallus

Riparin III effect on the development of neural tube embryos of Gallus gallus

A Comparative Study of the Teratogenic Effects of Antiepileptic Drugs: Lamotrigine and Levetiracetam on Adult Albino Rats

Impact of Bisphenol A on neural tube development in 48‐hr chicken embryos

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