The effects of low level laser therapy on Staphylococcus aureus infected third-degree burns in diabetic rats

Ranjbar, Reza; Takhtfooladi, Mohammad Ashrafzadeh

doi:10.1590/s0102-865020160040000005

Cited by 22 publications

(22 citation statements)

References 30 publications

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“…24,16,39,22,23 Ranjbar and Takhtfooladi have reported the positive effects of LLLT(685 nm, 5 sequential sessions, 40 J/cm 2 ) on wound contraction and wound strength in third-degree burn wounds that were infected with S. aureus in TIDM rats. 24 They reported average bacterial count in the experimental group was significantly lower than that in the control group on day 21. 24 Asghari et al reported positive influences of LLLT [or photobiomodulation (PBM), 890 nm, 80 Hz, 300 s duration, 0.324 J/cm 2 energy density] on the microbial flora and tensiometerical parameters of wounds in a nongenetic model of TIIDM compared to the placebo and metformin groups.…”

Section: Discussionmentioning

confidence: 99%

“…PW LLLT accelerated the wound‐healing process in streptozotocin (STZ)‐induced TIDM, as measured by tensiometerical parameters and histological characteristics, and significantly reduced the colony‐forming units (CFUs) of bacteria in non‐genetic rat models of Types 1 and 2 . In 2016, Ranjbar and Takhtfooladi reported positive effects of CWLLLT (685 nm, 3 J/cm 2 , 5 days) on infected burns with MRSA in TIDM animals …”

Section: Introductionmentioning

confidence: 99%

“…13,15,16 In 2016, Ranjbar and Takhtfooladi reported positive effects of CWLLLT (685 nm, 3 J/cm 2 , 5 days) on infected burns with MRSA in TIDM animals. 24 Mesenchymal stem cells (MSCs) release growth factors called secrotomes. 25 Secrotomes could be administered to wounds by a conditioned medium (CM).…”

Section: Introductionmentioning

confidence: 99%

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The effect of combined pulsed wave low‐level laser therapy and mesenchymal stem cell‐conditioned medium on the healing of an infected wound with methicillin‐resistant Staphylococcal aureus in diabetic rats

Kouhkheil

Fridoni

Piryaei

et al. 2018

J of Cellular Biochemistry

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This study aims to investigate the combined effects of Pulsed wave low-level laser therapy (PW LLLT) and human bone marrow mesenchymal stem cell-conditioned medium (hBM-MSC-CM) on the microbial flora and tensiometrical properties of an infected wound model with methicillin-resistant staphylococcal aureus (MRSA) in an experimental model for Type 1 diabetes mellitus (TIDM). TIDM was induced in rats by streptozotocin (STZ). One full-thickness excision was made on the backs of the rats. Next, the rats were divided into the following groups: Group 1 was the control (placebo) group; Group 2 received hBM-MSCs-CM four times; Group 3 were laser PWLLLT (890 nm, 80 Hz, 0.2 J/cm ); and Group 4 received hBM-MSCs-CM +LASER. Wounds were infected with MRSA. Microbiological examinations were performed on days 4, 7, and 15. Tensiometerical examinations were carried out on the 15th day. One-way analysis of variance showed that laser and CM alone and/or in combination significantly increases the tensiomerical properties of the repaired wounds compared with control wounds. A combination of PW laser and CM was statistically more effective than other treated groups. Two-way analysis of variance showed that laser and CM alone and/or in combination significantly decreases the colony-forming units (CFUs) compared with the control group. The application of hBM-MSC-CM and PWlaser alone and/or together significantly accelerates the wound-healing process in MRSA-infected cutaneous wounds in TI DM in rats. Additionally, a combined application of hBM-MSC-CM and PWlaser demonstrates a synergistic effect on the wound-healing process in MRSA-infected cutaneous wounds in Type I DM rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of combined pulsed wave low‐level laser therapy and mesenchymal stem cell‐conditioned medium on the healing of an infected wound with methicillin‐resistant Staphylococcal aureus in diabetic rats

Kouhkheil

Fridoni

Piryaei

et al. 2018

J of Cellular Biochemistry

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“…Novel approaches to treat MRSA contamination and counter the growing complication of MRSA are mandatory 34. Specifically, our experiment aimed to focus on the bactericidal effects of PBM47 and ADS34 because both have been individually reported to have bactericidal effects in animal models. To date, there is no probe that has examined the effects of PBM on immunomodulation of ADS.…”

Section: Discussionmentioning

confidence: 99%

Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Ebrahimpour-Malekshah

Amini

Zare

et al. 2020

BMJ Open Diab Res Care

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ObjectiveWe assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).Research design and methodsWe randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm2, energy density: 0.2 J/cm2) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA).ResultsAll three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups.ConclusionsCombination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1.

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“…Different course of antibiotic resistance, especially in methicillin resistant Staphylococcus aureus (MRSA) demand a new approach to management of innovative treatments [3]. To manage medical treatment of infected wounds, lots of agents and protocols have been proposed in the literature [4,5].…”

mentioning

confidence: 99%

Effects of Aloe Vera and Chitosan Nanoparticle Thin-Film Membranes on Wound Healing in Full Thickness Infected Wounds with Methicillin Resistant Staphylococcus Aureus

Ranjbar

Yousefi

2018

BEAT

Self Cite

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Objective: To assess effect of Aleo vera with chitosan nanoparticle biofilm on wound healing in full thickness infected wounds with antibiotic resistant gram positive bacteria. Method: Thirty rats were randomized into five groups of six rats each. Group I: Animals with uninfected wounds treated with 0.9% saline solution. Group II: Animals with infected wounds treated with saline. Group III: Animals with infected wounds were dressed with chitosan nanoparticle thin-film membranes. Group IV: Animals with infected wounds were treated topically with Aloe vera and Group V: Animals with infected wounds were treated topically with Aloe vera and dressed with chitosan nanoparticle thin-film membranes. Wound size was measured on 6, 9, 12, 15, 18 and 21days after surgery. Results: Microbiology, reduction in wound area and hydroxyproline contents indicated that there was significant difference (p<0.05) between group V and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (p<0.05) between group V and other groups. Conclusion: The Aloe vera with chitosan nanoparticle thin-film membranes had a reproducible wound healing potential and hereby justified its use in practice.

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The effects of low level laser therapy on Staphylococcus aureus infected third-degree burns in diabetic rats

Abstract: The low-level laser improved the healing of S. aureus third-degree burn infections in diabetic rats.

Cited by 22 publications

References 30 publications

The effect of combined pulsed wave low‐level laser therapy and mesenchymal stem cell‐conditioned medium on the healing of an infected wound with methicillin‐resistant Staphylococcal aureus in diabetic rats

The effect of combined pulsed wave low‐level laser therapy and mesenchymal stem cell‐conditioned medium on the healing of an infected wound with methicillin‐resistant Staphylococcal aureus in diabetic rats

Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Effects of Aloe Vera and Chitosan Nanoparticle Thin-Film Membranes on Wound Healing in Full Thickness Infected Wounds with Methicillin Resistant Staphylococcus Aureus

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