The effects of lycopene on methotrexate-induced liver injury in rats

Abstract

BACKGROUND: The aim of this study was to investigate the effects of lycopene (Lyc) on methotrexate (Mtx) induced liver toxicity in rats. METHODS: Twenty-eight male Sprague-Dawley rats were divided into four equal groups: control, Lyc, Mtx and Mtx-L: Control group: Rats were given only the vehicle. Lyc group: Rats were given Lyc (10 mg/kg) with corn oil by oral gavage for ten days. Mtx group: Rats were injected intraperitoneally with a single dose of 20 mg/kg of Mtx and given corn oil by oral gavage. Mtx-L group: Rats were post-treated with Lyc (10 mg/kg) for ten days after a single dose of Mtx (20 mg/kg). RESULTS: Mtx administration increased histopathological damage, TNF-α, IL-1β, TOS, TAS and OSI levels in tissues; AST, ALT levels in the blood. Sinusoidal dilatation, infl ammatory cell infi ltration and congestion were signifi cantly improved in the Mtx-L aon histopathologic examination of the rats. In Mtx-L group that were treated at the Lyc, TNF-α and IL-1β levels of liver tissue were decreased signifi cantly compared to Mtx group whereas the decrease in OSI was not signifi cant. Lyc treatment improved the AST and ALT values in Mtx-L group. But only AST improvement was signifi cant. CONCLUSIONS: The results of this study revealed that Lyc might be useful in protecting the liver from injury due to Mtx in rats by reducing the increased proinfl ammatory cytokine levels (Tab. 4, Fig. 1, Ref. 44). Text in PDF www.elis.sk.

The Effect of Pretreatment With Toll-Like Receptor 4 Antagonist Resatorvid on Methotrexate-Induced Liver Injury in Rats: Histopathological Study

“…Moderate inflammation observed in the histological section of treated animals with infiltration of chronic inflammatory cells. The histopathological changes we achieved in this study are in consistent with the previous studies, which described severe focal necrosis accompanied by hepatic granular damage, abnormal hepatocytes rearrangement around central vein with shrunken and inflected nuclei, infiltration of mononuclear cells, vascular congestion, increased KCs activation and proliferated connective tissue in hepatic sections of those rats treated with MTX [15,20,21,34,[41][42][43]. Despite, our finding represent the moderate-severe patterns of MTX-induced liver injury and thus we described milder steatohepatitic changes [11,31,34,39,40].…”

“…A total of 28 male albino Wistar rats (aged 4-6 mo and weighed 125-225 g) taken from Kut Technical Institute, University of Wasit, were maintained under non-specific pathogen-free conditions in wiremeshed cages (7 rats in each cage) with ad libitum access to water and regular diet. The animals were kept under a constant temperature 24 ± 3°C with 12:12 h light dark cycle [13][14][15]. Animal handling and housing have proceeded in accordance with the international guidelines for the care and use of laboratory animals of the National Research Council [13,14].…”

The effect of resveratrol on oxidative ovary-damage induced by methotrexate in rats (Resveratrol oxidative ovary-damage)

“…One hour after resveratrol and distilled water was administered, RMTX and MTX rat groups were injected with 2 mg/kg methotrexate intraperitoneally (i.p.). The average dose of methotrexate to produce oxidative stress in rats is 10-50 mg/kg (Omer et al 2014;Yucel et al 2017). This procedure was repeated once a day for 30 days.…”

An experimental study on the use of lycopene to prevent infertility due to acute oxidative ovarian damage caused by a single high dose of methotrexate

“…with a single dose of MTX (20 mg/kg). 2 The lycopene and the solvent were administered once a day for 5 days. At the end of this application, 6 rats from each group (MG, LMG and HCG) were euthanized with a high dose of anesthesia (50 mg/kg of thiopental sodium i.p.)…”

“…1 It has been found that this agent, which is also frequently used in ectopic and molar pregnancy treatments, increases the level of free oxygen radicals and pro-inflammatory molecules. 2 It is also gonadotoxic and decreases the number of ovarian follicles. In this way, it is thought to cause infertility, especially in young patients.…”