The effects of male age on sperm DNA damage: an evaluation of 2,178 semen
            samples

Comar, V.A.; Ricci, Juliana; Dieamant, Felipe; Oliveira, J.B.A.; Baruffi, R.L.R.; Júnior, Jose G. Franco

doi:10.5935/1518-0557.20180047

Cited by 29 publications

(28 citation statements)

References 61 publications

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“…More studies with greater numbers of normal fertile individuals must be performed to confirm the effects of aging on sperm concentration, total sperm count, sperm motility, and sperm DNA fragmentation. Nevertheless, the findings reported in this and other similar studies (Varshini et al, 2012;Stone et al, 2013;Oliveira et al, 2014;Gunes et al, 2016;Petersen et al, 2018) suggest that aging has a negative effect on most semen parameters and DNA integrity. Therefore, age is an important factor to consider in the treatment of infertile couples.…”

Section: Discussionsupporting

confidence: 41%

“…Other authors using the same technique described similar findings (Varshini et al, 2012;Oliveira et al, 2014). Petersen et al (2018) also concluded that DNA fragmentation worsened with age and associated it with mitochondrial damage, since mitochondrial membrane potentials deteriorate significantly with age. One might argue that it is advisable to assess older men seeking fertility treatment for DNA fragmentation, since it may cause infertility (Ahmadi et al, 2016) and increase miscarriage rates (García-Ferreyra et al, 2015).…”

Section: Discussionmentioning

confidence: 53%

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The effects of aging on semen parameters and sperm DNA fragmentation

Pino¹,

Sanz²,

Valdés³

et al. 2020

JBRA Assisted Reproduction

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Introduction:This study aimed to look into possible correlations between male age and different sperm parameters derived from semen analysis and sperm deoxyribonucleic acid (DNA) fragmentation.Methods: This retrospective descriptive study included 2681 male patients who underwent semen analysis at Clínica Las Condes (CLC), Santiago, Chile, between January 2014 and May 2017; correlations between age and sperm parameters were analyzed.Results: Males above the age of 50 were significantly more likely to present anomalies in semen volume, sperm concentration, and sperm DNA fragmentation; males aged 41+ years were more likely to have lower sperm concentration levels; males aged 31+ years were more likely to have decreased sperm motility; when concentration was constant, more volume and motility anomalies were seen as age increased; when volume was kept constant, more motility and concentration anomalies were seen as age increased; and when motility was constant, normal semen volumes decreased as age increased.Conclusion: Our study showed that male age significantly affects sperm parameters that might have an impact on male fertility.

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Section: Discussionsupporting

confidence: 41%

Section: Discussionmentioning

confidence: 53%

The effects of aging on semen parameters and sperm DNA fragmentation

Pino¹,

Sanz²,

Valdés³

et al. 2020

JBRA Assisted Reproduction

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show abstract

“…Our study population was probably too small to adequately address the question of whether or not lifestyle factors, such as BMI, alcohol and smoking may also have an influence on acrosomal status. Male age has been found to have an effect on some of the classical sperm parameters and sperm DNA fragmentation [ 27 , 28 , 29 ]; however, we were not able to show a significant correlation between acrosomal status and the age of the male partner, which again may be due to the size of study population. The age of the female partner has a significant impact on the chances of pregnancy and also on the type of fertility treatment chosen [ 30 , 31 ].…”

Section: Discussioncontrasting

confidence: 97%

“…Earlier studies on the importance of the acrosome are mainly related to the acrosomal responsiveness, i.e., the ability of the acrosome to react to stimuli, such as an ionophore or progesterone, and release its content by exocytosis [ 25 , 26 , 27 , 28 ]. Acrosomal responsiveness is, however, a different matter of concern as it only relates to the proportion of spermatozoa that still retain an intact acrosome.…”

Section: Discussionmentioning

confidence: 99%

The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

Norup

Palme²,

Petersen

et al. 2020

JCM

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The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) (n = 99) and intracytoplasmic sperm injection (ICSI) (n = 107) treatment. Purified frozen-thawed donor samples were also analyzed (n = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors (p = 5.3 × 10−8) and men from IVF couples (p = 2.2 × 10−5) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility (p = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, p = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.

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“…С помощью метода TUNEL в ряде работ выявлена прямая зависимость уровня фрагментации ДНК от возраста мужчин [4][5][6][7], что согласуется с данными, полученными в нашем исследовании. Другие авторы не выявили кор реляции с целостностью хроматина сперматозоидов [8,9].…”

Section: Discussionunclassified

Sperm DNA fragmentation in men of different age

Хаят

Брагина

Arifulin

et al. 2019

Androl. genit. hir.

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The study objective is to analyze the content of spermatozoa with single and double-stranded DNA breaks in different age groups.Materials and methods. The level of DNA fragmentation was studied in 300 ejaculate samples obtained from 266 sub- or infertile men. The group 1 included 150 samples obtained from 131 patients under the age of 45 (21–44 years), the group 2 included 150 samples obtained from 135 patients above the age of 45 (45–68 years). Mean ages were 34.8 ± 3.9 and 48.6 ± 3.1 years, respectively. The number of sperm with fragmented DNA was evaluated using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method on ejaculate smears. The number of spermatozoa with >15 % of fragmented DNA was considered elevated. Standard semen analysis was performed in 117 and 97 men from the groups 1 and 2, respectively.Results. The number of sperm with fragmented DNA varied in ejaculated samples from 1.5 to 64.5 %. Mean number of sperm with DNA breaks in the group 1 (12.0 ± 6.0 %) was significantly lower than in the group 2 (16.1 ± 8.3 %, p <0.05). Mean sperm count in the ejaculate of the group 1 (267.0 ± 198.7 million) was significantly higher than in the group 2 (201.0 ± 162.9 million, p = 0.02).Conclusion. We revealed that in men over the age of 45 years, the percentage of spermatozoa with DNA fragmentation is higher than in men under 45 years of age, it may indirectly indicate an increased level of reactive oxygen species in the seminal plasma in older patients.

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The effects of male age on sperm DNA damage: an evaluation of 2,178 semen samples

Cited by 29 publications

References 61 publications

The effects of aging on semen parameters and sperm DNA fragmentation

The effects of aging on semen parameters and sperm DNA fragmentation

The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

Sperm DNA fragmentation in men of different age

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