The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (<i>Citrus sinensis</i>[L.] Osbeck ×<i>Poncirus trifoliata</i>[L.] Raf.)

Chiancone, Benedetta; Martorana, Letizia; Gianguzzi, Valeria; Germana, Maria

doi:10.17660/actahortic.2017.1155.26

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…The results obtained in the present study allow us to highlight the comparative value of TIS and solid medium during the multiplication phase of three different caper selected accessions cultivated in the Aeolian island of Salina. As a whole, we observed a higher RGR in TIS compared to the solid medium system, together with better proliferation indices and higher number of shoots produced by the explants, in accordance with previous results obtained for other plant species [46,47,48,49]. Bioreactor cultures have several advantages compared to those on agar substrate, such as a better control of plant tissue contact with the culture medium, optimal supply of nutrients and growth regulators, as well as better aeration and substrate circulation, filtration of the medium, and reduction of the number of cultures.…”

Section: Discussionsupporting

confidence: 92%

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In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System

Gianguzzi

Inglese

Barone

et al. 2019

Plants

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Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38°33′49″ N) were micropropagated to evaluate two different in vitro culture systems: one using the traditional solid medium, and the other based on liquid culture in a PlantForm bioreactor. PlantForm is a temporary immersion system (TIS), a new propagation method in which the shoots undergo temporary immersion in a liquid medium in order to avoid the accumulation of gas through forced ventilation. This study proposes a protocol to improve the efficiency of in vitro propagation of caper plants, while also reducing production costs, because of the elimination of the gelling agent, and manual labor, requiring limited subcultures and posing minimal contamination risks. Our results show that the caper shoots propagated in bioreactors demonstrated good adaptability and better growth rates than those grown in the conventional system. Statistically significant differences were observed between plants grown in the PlantForm liquid culture and those grown in solid medium regarding the number and length of shoots, which were further promoted by the addition of plant growth regulators (PGRs). The relative growth and real proliferation rate of the caper explants were higher when using meta-Topolin than when using 6-benzylaminopurine as a PGR. Overall, the TIS improved in vitro caper culture by promoting the proliferation, length, and vigor of the shoots.

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Section: Discussionsupporting

confidence: 92%

“…In the present study, the best results were obtained in the MS medium integrated with meta-Topolin. The efficiency of mT has also been reported for a number of plant species different from caper [44,45,46,47].…”

Section: Resultsmentioning

confidence: 82%

In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System

Gianguzzi

Inglese

Barone

et al. 2019

Plants

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“…For these reasons, new aromatic derivatives of cytokinin have been intensively researched [66]. One such derivative is mT, a highly active natural aromatic cytokinin widely used in the micropropagation of various species [56,68,69].…”

Section: Discussionmentioning

confidence: 99%

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Gianguzzi,

Sottile

2024

Horticulturae

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The genus Sorbus has maintained an extremely relevant role over time from a landscape and environmental perspective in many countries in the Mediterranean and Central Europe. Based on the requirements coming from the environmental policies provided in the European strategy Next Generation EU, Sorbus has been considered a valuable species to be introduced in urban and peri-urban areas. The purpose of this study was to propagate four Sorbus accessions selected in the Sicilian territory, Southern Italy, using the liquid substrate in temporary immersion bioreactors Plantform™. The results obtained showed that the presence of 1 mg L−1 mT in the substrate in combination with IBA 0.05 mg L−1 produced a significant number of shoots (4.7) and a greater length (2.2 cm). Among the accessions, there were statistically significant differences; the accession SN2 and SN1 produced more shoots (respectively, 4.0 and 3.6), and a greater length of the shoots was observed in the selections SN4 and SN3 (respectively, 2.4 cm and 2.3 cm). The relative growth rate (RGR) was significantly influenced by the presence of the culture substrate of the combination of cytokines and auxin; SN4 selection showed the best RGR results of 8.3 mg−1 d−1. The use of the bioreactor Plantform™ in Sorbus domestica L. has favored a better development of plants obtained in vitro, demonstrating that this system is a valid alternative for the micropropagation of Sorbus.

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Role of Meta-topolin on in Vitro Shoot Regeneration: An Insight

Gantait

Mitra

2021

Meta-Topolin: A Growth Regulator for Plant Biotechnology and Agriculture

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The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (Citrus sinensis[L.] Osbeck ×Poncirus trifoliata[L.] Raf.)

Cited by 4 publications

References 31 publications

In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System

In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Role of Meta-topolin on in Vitro Shoot Regeneration: An Insight

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