The effects of mineral trioxide aggregate on osteo/odontogenic potential of mesenchymal stem cells: a comprehensive and systematic literature review

Babaki, Danial; Yaghoubi, Sanam; Matin, Maryam Moghaddam

doi:10.1080/26415275.2020.1848432

Cited by 7 publications

(6 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among root canal filling materials, investigators have widely reported the successful application of mineral trioxide aggregate (MTA) in vital pulp therapy [ 16 ]. Moreover, bioactivity and cytotoxicity measurements revealed that MTA not only shows biocompatibility toward bone marrow and dental-derived mesenchymal stem cells but also can induce their proliferation and osteo/odontoblast-like differentiation in a concentration-dependent manner [ 6 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

MTA Enhances the Potential of Adipose-Derived Mesenchymal Stem Cells for Dentin–Pulp Complex Regeneration

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of the current study was to investigate the effects of mineral trioxide aggregate (MTA) on the proliferation and differentiation of human adipose-derived mesenchymal stem cells (Ad-MSCs) as a surrogate cell source in futuristic stem-cell-based endodontic therapies. Human Ad-MSCs and mesenchymal stem cells derived from bone marrow (BM-MSCs) were isolated from liposuction waste adipose tissue and femur, respectively, and the effects of MTA-conditioned media on their viability, mineralization potential, and osteo/odontogenic differentiation capacity were subsequently evaluated. Alkaline phosphatase (ALP) activity, quantitative alizarin red S staining, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses were performed to investigate and compare the osteo/odontogenic induction potential of MTA on the Ad/BM-MSCs. The results of cytotoxicity assay revealed that at different concentrations, MTA-conditioned medium was not only biocompatible toward both cell types, but also capable of promoting cell proliferation. ALP activity assay showed that 0.2 mg/mL was the optimal concentration of MTA-conditioned medium for osteo/odontogenic induction in Ad/BM-MSCs. The expression of osteo/odontogenic gene markers was increased in Ad/BM-MSCs treated with 0.2 mg/mL MTA-conditioned media. Our results indicated that MTA can efficiently enhance the osteo/odontogenic potential of Ad-MSCs, and thus they can be considered as a better cell source for dentin–pulp complex regeneration. However, further investigations are required to test these potentials in animal models.

show abstract

Section: Introductionmentioning

confidence: 99%

MTA Enhances the Potential of Adipose-Derived Mesenchymal Stem Cells for Dentin–Pulp Complex Regeneration

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…TheraCal PT ® was the least performing tested material in the present study and is in alignment with previous studies, which proved MTA and Biodentine™ had superior regenerative powers when compared to TheraCal LC ® (Farsi et al, 2018;Kang, 2020;Manaspon et al, 2021;Omidi et al, 2020). This was further supported by Babaki et al (2020) who concluded that MTA is promising with regard to stem-cell-based endodontic approaches due to its osteo/odontogenic differentiation-inducing properties, but further animal/human trials were deemed necessary. Despite TheraCal PT ® showing superior properties to TheraCal LC ® (Sanz et al, 2021), it seems that the TheraCal family of pulp-capping agents failed to outperform other materials as shown in the present study.…”

Section: Pcr Resultsmentioning

confidence: 90%

“…An ideal pulp capping material should prevent bacterial infiltration, trigger minimal inflammation, and induce dentin bridge formation (Qureshi et al, 2014 ). Current materials clinically used for pulp capping can be generally divided into calcium hydroxide (Ca(OH) 2 ) (Peng et al, 2022 ; Whitehouse et al, 2021 ; Youssef et al, 2019 ), mineral trioxide aggregate (MTA) (Babaki et al, 2020 ; Youssef et al, 2019 ), calcium silicate (Kang, 2020 ; Kim et al, 2021 ; Onay et al 2018 ), and adhesive‐based materials (Giraud et al, 2019 ). Interestingly, MTA and calcium silicate‐based materials have a comparable effect on dentin bridge formation, inflammatory response, and success rate (Babaki et al, 2020 ; Paula et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Current materials clinically used for pulp capping can be generally divided into calcium hydroxide (Ca(OH) 2 ) (Peng et al, 2022 ; Whitehouse et al, 2021 ; Youssef et al, 2019 ), mineral trioxide aggregate (MTA) (Babaki et al, 2020 ; Youssef et al, 2019 ), calcium silicate (Kang, 2020 ; Kim et al, 2021 ; Onay et al 2018 ), and adhesive‐based materials (Giraud et al, 2019 ). Interestingly, MTA and calcium silicate‐based materials have a comparable effect on dentin bridge formation, inflammatory response, and success rate (Babaki et al, 2020 ; Paula et al, 2018 ). Apart from these clinical observations, direct comparison of the effects of these materials on hDPSCs in vitro is limited.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of pulp capping materials on odontogenic differentiation of human dental pulp stem cells: An in vitro study

Bakr,

Shamel,

Raafat

et al. 2023

Clinical & Exp Dental Res

View full text Add to dashboard Cite

ObjectivesMigration and differentiation of human dental pulp stem cells (hDPSCs) is a vital and key factor in the success of reparative dentin formation for maintenance of pulp vitality. Pulp capping materials are used to stimulate DPSCs to induce new dentin formation. Thus, the aim of the present study was to compare the response of DPSCs to four commercially available pulp capping materials: a bioactive bioceramic (Material 1), a nonresinous ready‐to‐use bioceramic cement (Material 2), a bioactive composite (Material 3), and a biocompatible, dual‐cured, resin‐modified calcium silicate (Material 4).Materials and MethodshDPSCs were isolated and cultured from freshly extracted teeth and were then characterized by flow cytometry and multilineage differentiation. Discs prepared from pulp capping materials were tested with hDPSCs and MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay, cell migration assay and odontogenic differentiation assay was performed. Expression of osteogenic markers (osteopontin, RUNX family transcription factor 2, osteocalcin) and the odontogenic marker (dentin sialophosphoprotein) was detected using reverse transcription‐polymerase chain reaction.ResultsMaterials 1, 2, and 3 generated more cell viability than Material 4. Furthermore, Material 4 showed the least wound exposure percentage, while Material 3 showed the highest percentage. Enhanced mineralization was found in hDSCPs cultured with Material 3, followed by Material 1, and then Material 2, while Material 4 revealed the least calcified mineralization.ConclusionsThe results of this study were inconclusive regards contemporary bioceramic materials designed for vital pulp therapy as they have different effects on hDPSC. Further testing for cytotoxicity using live‐dead staining, animal experiments, clinical trials, and independent analyses of these biomaterials is necessary for clinicians to make an informed decision for their use.

show abstract

“…Various MTA materials were tested on bone marrow-derived MSCs and were demonstrated to be non-toxic, but they could affect tooth-derived stem cells in a concentration and time-dependent manner [18]. Studies indicate that MTA materials can promote the proliferation and survival of MSCs derived from dental tissues including apical papilla, exfoliated deciduous teeth, and dental pulp [19].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and Osteo/Odontogenic Potential of Various Bioactive Root-End Filling Materials

Ercal,

Sismanoglu

2023

Applied Sciences

View full text Add to dashboard Cite

This in vitro study aimed to investigate if different bioactive root-end filling materials can promote osteo/odontogenic differentiation of mesenchymal stem cells (MSCs) and support their viability. MSCs from porcine tooth germs were isolated. Cells were exposed to extracts from MTA Angelus, BIOfactor MTA, Medcem MTA, Well-Root ST, and Pure Portland Cement for 7 days. Viability was determined with MTS and live/dead assay. Osteo/odontogenic differentiation was evaluated with alkaline phosphatase (ALP) activity and quantitative real-time PCR (RUNX2, DMP-1, and DSPP genes) which were compared with osteo/odontogenic-induced MSCs and non-treated cells. All the tested materials supported cell proliferation and cells maintained their viability after 7 days. Osteo/odontogenic differentiation of MSCs was promoted by the tested materials in varying levels as demonstrated by increased ALP activity and upregulation of related gene markers in comparison to the control group. Pure Portland Cement demonstrated a continuous high ALP activity on day 7, showing the highest value among all materials and significantly increased in comparison to the control group (p < 0.001). Significant RUNX2 expression and high ALP activity (p < 0.001) similar to that of osteogenically induced cells was detected for Pure Portland Cement after 7 days. Tested MTA-based cement materials are biocompatible and induce osteo/odontogenic differentiation in vitro. MTA materials performed similarly to Pure Portland Cement regarding osteo/odontogenic differentiation.

show abstract

The effects of mineral trioxide aggregate on osteo/odontogenic potential of mesenchymal stem cells: a comprehensive and systematic literature review

Cited by 7 publications

References 91 publications

MTA Enhances the Potential of Adipose-Derived Mesenchymal Stem Cells for Dentin–Pulp Complex Regeneration

MTA Enhances the Potential of Adipose-Derived Mesenchymal Stem Cells for Dentin–Pulp Complex Regeneration

Effect of pulp capping materials on odontogenic differentiation of human dental pulp stem cells: An in vitro study

Biocompatibility and Osteo/Odontogenic Potential of Various Bioactive Root-End Filling Materials

Contact Info

Product

Resources

About