The effects of multiple freeze–thaw cycles on the biomechanical properties of the human bone‐patellar tendon‐bone allograft

Jung, Hosung; Vangipuram, Gautum; Fisher, Matthew B.; Yang, Guoguang; Hsu, Shan-Ling; Bianchi, John; Ronholdt, Chad J.; Woo, Savio L‐Y.

doi:10.1002/jor.21373

Cited by 99 publications

(84 citation statements)

References 26 publications

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“…The material parameters, stress, and modulus were unaffected by increasing freeze-thaw cycles or even by freeze-drying. These results are in line with those of other authors regarding soft tissue [6,8,9] and hard tissue [10,13,14] as a result of freezing and thawing cycles. Two previous studies [12,17] found that processing and storage caused freeze-dried allograft bone to be weaker, but we did not compare our samples with the same control group.…”

Section: Discussionsupporting

confidence: 93%

“…These specific treatments were chosen to span the range between the minimum and maximum cycles expected to be encountered by graft material between recovery and use. The maximum number of eight freeze-thaw cycles was chosen by consultation with American Association of Tissue Banks [1] and other published data [6]. Each treatment was represented once within a donor, thus each treatment had an n = 18.…”

Section: Methodsmentioning

confidence: 99%

“…Another found four freeze-thaw cycles decreased the ability of meniscal allografts to resist compressive forces [9]. A third, more-recent study reported that as many as eight freeze-thaw cycles do not affect the biomechanical properties of bone-patellar tendon-bone grafts [6]. However, it is difficult to ascertain the effect of freeze-thaw cycles on bone using data derived from soft tissue, and studies on allograft cortical bone are limited and differ in their findings.…”

Section: Introductionmentioning

confidence: 99%

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Repeated Freeze-thaw Cycles Do Not Alter the Biomechanical Properties of Fibular Allograft Bone

Shaw¹,

Hunter²,

Gayton

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Background Allograft tissues can undergo several freezethaw cycles between donor tissue recovery and final use by surgeons. However, there are currently no standards indicating the number of reasonable freeze-thaw cycles for allograft bone and it is unclear how much a graft may be degraded with multiple cycles.Questions/purposes We therefore asked whether (1) the mechanical properties of fibular allograft bone would remain unchanged with increasing numbers of freeze-thaw cycles and (2) histologic alterations from increased numbers of freeze-thaw cycles would correspond to any mechanical changes. Methods Fibular allograft segments were subjected to two, four, and eight freeze-thaw cycles and compared biomechanically and histologically with a control group (one freeze-thaw cycle). Two freeze-dried treatments, one after being subjected to one freeze-thaw cycle and the other after being subjected to three freeze-thaw cycles, also were compared with the control group. Results For all segments, the average ultimate stress was 174 MPa, average modulus was 289 MPa, average energy was 2.00 J, and the average stiffness was 1320 N/mm. The material properties of the freeze-thaw treatment groups were similar to those of the control group: ultimate stress and modulus were a maximum of 16% and 70% different, respectively. Both freeze-dried treatments showed increased stiffness (maximum 53% ± 71%) and energy to failure (maximum 117% ± 137%) but did not exhibit morphologic differences. There were no alterations in the histologic appearance of the bone sections in any group. Conclusions Fibular allograft segments can be refrozen safely up to eight times without affecting the biomechanical or morphologic properties. Freeze-dried treatments require further study to determine whether the detected differences are caused by the processing. Clinical Relevance Cryopreserved cortical allografts are thawed by surgeons in preparation for procedures and then occasionally discarded when not used. Refreezing allograft tissues can result in a cost savings because of a reduction in wasted graft material.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Repeated Freeze-thaw Cycles Do Not Alter the Biomechanical Properties of Fibular Allograft Bone

Shaw¹,

Hunter²,

Gayton

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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“…As radiografias foram avaliadas quanto a doença óssea ou nutricional, fraturas da crista da tíbia, sinais de artroplastia ou osteotomia da tuberosidade da tíbia. Após remoção de tecidos moles estas foram armazenadas em freezer convencional, com temperatura de -20° Celsius (PARK et al, 2009;GOH et al, 2010;JUNG et al, 2011). As peças foram armazenadas em embalagens plásticas com dimensões de 50 centímetros de altura e 30 centímetros de largura.…”

Section: Limitações Do Estudounclassified

“…(JUNG et al, 2011). Esta temperatura foi baseada em estudos similares previamente realizados (DENNLER et al, 2006;KOWALESKY;LINS et al, 2009;PARK et al, 2009;ETCHEPAREBORDE et al, 2010;GOH et al, 2010;BUTLER et al, 2011;JUNG et al, 2011 DOVERSPIKE et al, 1993;DUPUIS et al, 1994;CHAUVET et al, 1996;BONFIM;PACCOLA, 2000;INNES et al, 2000;MORRIS;LIPOWITZ, 2001;BOUDRIEAU, 2005;CONZEMIUS et al, 2005;DAMUR, 2005;HOFFMANN et al, 2006;BARAÚNA et al, 2007;TARTARUNAS et al, 2007;STEINS;SCHMOEKEL, 2008;VOSS, et al, 2008;GUERRERO et al, 2009;DYMOND;GOLDSMID;SIMPSON, 2010;BISGARD et al, 2011;KEMPER et al, 2011;STEINBERG et al, 2011).…”

Section: Dispositivo Para Corpo De Provaunclassified

Estudo biomecânico de tração (ex-vivo) em osteotomia da tuberosidade tibial de cães estabilizada com placa fixa por "garfo" ou parafuso

Ito¹

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Agradeço ao meu orientador, Professor Cassio Ricardo Auada Ferrigno, a quem devo este trabalho. Palavras não são suficientes para agradecer os últimos 6 anos que passaram. Foram 6 anos de muito trabalho, conquistas e oportunidades em minha vida; ingressar na pós-graduação da Universidade de São Paulo era um sonho muito muito distante que pôde se tornar realidade. Obrigada professor Cassio por ter me aceito como orientada, por tudo que fez por mim, pelos ensinamentos profissionais e pessoais, aprendi tudo que sei dentro da ortopedia veterinária com o senhor e espero não tê-lo decepcionado. Obrigada pela confiança e por permitir que possa ter a honra de dizer daqui para frente que fui sua orientada.Obrigada a meus pais José Mutuo Ito e Rosa Sanae Ito por todo apoio em todos os momentos e por terem feito de mim uma pessoa de caráter.Obrigada a meus irmãos Edson Massao Ito, Katiuscia Namie Ito Hiroto e seus familiares por estarem presentes na minha vida e pelo apoio nos momentos que precisei.Ao meu noivo Leonardo Seizen Yamauchi e sua família agradeço pelo amor, companheirismo e muita paciência que teve durante este período.Ao amigo César Augusto Martins Pereira pelos desenhos dos dispositivos, pela prontidão nos momentos de dificuldade e pela ajuda incondicional na realização dos ensaios biomecânicos sem a qual não teria conseguido.Aos meus queridos amigos de trabalho... dos anciãos para os mais novos: Marcos

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Effects of freezing/thawing on the mechanical properties of decellularized lungs

Nonaka

Campillo

Uriarte

et al. 2013

J Biomedical Materials Res

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Lung bioengineering based on decellularized organ scaffolds is a potential alternative for transplantation. Freezing/thawing, a usual procedure in organ decellularization and storage could modify the mechanical properties of the lung scaffold and reduce the performance of the bioengineered lung when subjected to the physiological inflation-deflation breathing cycles. The aim of this study was to determine the effects of repeated freezing/thawing on the mechanical properties of decellularized lungs in the physiological pressure-volume regime associated with normal ventilation. Fifteen mice lungs (C57BL/6) were decellularized using a conventional protocol not involving organ freezing and based on sodium dodecyl sulfate detergent. Subsequently, the mechanical properties of the acellular lungs were measured before and after subjecting them to three consecutive cycles of freezing/thawing. The resistance (RL ) and elastance (EL ) of the decellularized lungs were computed by linear regression fitting of the recorded signals (tracheal pressure, flow, and volume) during mechanical ventilation. RL was not significantly modified by freezing-thawing: from 0.88 ± 0.37 to 0.90 ± 0.38 cmH2 O·s·mL(-1) (mean ± SE). EL slightly increased from 64.4 ± 11.1 to 73.0 ± 16.3 cmH2 O·mL(-1) after the three freeze-thaw cycles (p = 0.0013). In conclusion, the freezing/thawing process that is commonly used for both organ decellularization and storage induces only minor changes in the ventilation mechanical properties of the organ scaffold.

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The effects of multiple freeze–thaw cycles on the biomechanical properties of the human bone‐patellar tendon‐bone allograft

Cited by 99 publications

References 26 publications

Repeated Freeze-thaw Cycles Do Not Alter the Biomechanical Properties of Fibular Allograft Bone

Repeated Freeze-thaw Cycles Do Not Alter the Biomechanical Properties of Fibular Allograft Bone

Estudo biomecânico de tração (ex-vivo) em osteotomia da tuberosidade tibial de cães estabilizada com placa fixa por "garfo" ou parafuso

Effects of freezing/thawing on the mechanical properties of decellularized lungs

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