The Effects of Orientin on Proliferation and Apoptosis of T24 Human Bladder Carcinoma Cells Occurs Through the Inhibition of Nuclear Factor-kappaB and the Hedgehog Signaling Pathway

Tian, Fei; Tang, Ming; Li, Zizhi; Huang, Weichao; Jin, Yanyang; Cao, Qingfei; Zhou, Xiaoli; Tong, Guangquan

doi:10.12659/msm.919203

Cited by 22 publications

(12 citation statements)

References 31 publications

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“…Induced apoptosis and altered apoptotic protein levels [393,394]. Caused cell cycle arrest [394,395]. Decreased cell proliferation [393,395].…”

Section: Orientinmentioning

confidence: 99%

“…In these cells, orientin induced apoptosis and cell cycle arrest at the G0/G1 phase through the regulation of cyclin and cyclin-dependent protein kinases [ 394 ]. Additionally, orientin inhibited T24 human transitional cell bladder carcinoma cell proliferation, induced cell cycle arrest, and decreased the expression of inflammatory mediators such as NF-ĸB and components of the Hedgehog signaling pathway, at a concentration of 100 µM [ 395 ]. In vivo, orientin administration (10 mg/kg) resulted in antiproliferative effects on 1,2-dimethyl hydrazine (DMH)-induced colorectal cancer in rats, and improved tumor marker levels while decreasing proliferative marker levels, such as proliferating cell nuclear antigen (PCNA) and Ki67 [ 396 ].…”

Section: Flavonoidsmentioning

confidence: 99%

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Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis

Tomko

Whynot

Ellis

et al. 2020

Cancers

162

139

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In recent years, and even more since its legalization in several jurisdictions, cannabis and the endocannabinoid system have received an increasing amount of interest related to their potential exploitation in clinical settings. Cannabinoids have been suggested and shown to be effective in the treatment of various conditions. In cancer, the endocannabinoid system is altered in numerous types of tumours and can relate to cancer prognosis and disease outcome. Additionally, cannabinoids display anticancer effects in several models by suppressing the proliferation, migration and/or invasion of cancer cells, as well as tumour angiogenesis. However, the therapeutic use of cannabinoids is currently limited to the treatment of symptoms and pain associated with chemotherapy, while their potential use as cytotoxic drugs in chemotherapy still requires validation in patients. Along with cannabinoids, cannabis contains several other compounds that have also been shown to exert anti-tumorigenic actions. The potential anti-cancer effects of cannabinoids, terpenes and flavonoids, present in cannabis, are explored in this literature review.

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“…Induced apoptosis and altered apoptotic protein levels [393,394]. Caused cell cycle arrest [394,395]. Decreased cell proliferation [393,395].…”

Section: Orientinmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis

Tomko

Whynot

Ellis

et al. 2020

Cancers

162

139

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“…e function of TCM in the procession of bladder cancer has been widely studied in recent years. For example, orientin repressed bladder cancer cell growth and facilitated cell apoptosis by downregulating NF-kappaB and Hedgehog pathway [19]. Curcumin inhibited bladder cancer cell growth through multiple signaling pathways [20,21].…”

Section: Discussionmentioning

confidence: 99%

Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

Xu¹,

Dong

Hou

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer. Methods. The mRNA expression of HEIH in bladder cancer cells was measured by RT-qPCR. T24 and SW780 cells were treated with sinomenine for 24 hours. Cell viability was detected by the MTT assay. Cell migration and invasion were detected by the transwell assay. Western blotting assay was performed to assess the protein expression of Bcl-2, Bax, and caspase-3. Results. Sinomenine significantly suppressed cell viability in T24 and SW780 cells. Moreover, cell migration and invasion were significantly inhibited by sinomenine. Sinomenine accelerated the expression of Bax and caspase-3 but decreased the expression of Bcl-2. HEIH was upregulated in bladder cancer cells compared with normal bladder epithelial cells. Besides this, we noticed that HEIH knockdown blocked cell proliferation, migration, and invasion but facilitated cell apoptosis in bladder cancer cells. Additionally, HEIH reversed the suppression of the progression induced by sinomenine. Conclusion. Sinomenine was observed to suppress cell progression of bladder cancer cells by inhibiting HEIH expression. Our findings suggested that the use of sinomenine might be an effective treatment for bladder cancer.

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“…A recent report on the T24 cell line showed orientininduced G0-G1 and S phase cell cycle arrest by decreasing the Cyclin E1 and CDK2 expressions. Orientin treatment was also reported to significantly inhibit the NF-κB signaling pathway and down-regulated the protein expressions of the Hedgehog signaling pathway in human bladder cancer cell lines (Tian et al 2019).…”

Section: Orientinmentioning

confidence: 96%

Glycosidic flavonoids and their potential applications in cancer research: a review

Abusaliya

Bhosale

et al. 2021

Mol. Cell. Toxicol.

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Purpose of review Every year, the cancer patient registry increases, and the leading cause of death in a global context. Plant-based molecules are gaining attention in cancer research due to the side effects of chemotherapy. A glycosidic derivative of flavonoid (GDF) plays a significant role in cancer proliferation mechanisms. GDF inhibits cell proliferation by elevating the expression of apoptotic proteins, altering the expression of nuclear factor-kappa B (NF- κB), and decreasing mitochondrial membrane potential (Δψm) in cancer cells. Recent findings Reported studies on the flavonoids orientin, vitexin, prunetionoside, chrysin, and scutellarein increased attention and are being widely investigated for their potential role in different parts of cancer research. Prunetionoside is a flavonoid with high cytotoxic potential and capable of inducing necroptosis in AGS gastric cancer cells. Similarly, scutellarein is a flavonol, induces an extrinsic apoptotic pathway and downregulates the expression level of cyclin proteins in HepG2 liver cancer cells. Vitexin is reported to be capable of deregulating the expression levels of p-Akt, p-mTOR, and p-PI3K in A549 lung cancer cells. Orientin inhibits IL-8 expression and invasion in MCF-7 breast cancer cells by suppressing MMP-9 in the presence of TPA via STAT3/AP-1/ERK/PKCα-mediated signaling pathways. It also induces mitochondria-mediated intrinsic apoptosis and G0/G1 cell cycle arrest in HT29 colon cancer cells. Chrysin is a flavonoid present in honey that has been shown to play an important role in cervical and colon cancer by suppressing the AKT/mTOR/PI3K pathway and increasing ROS accumulation, LDH leakage, respectively.

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The Effects of Orientin on Proliferation and Apoptosis of T24 Human Bladder Carcinoma Cells Occurs Through the Inhibition of Nuclear Factor-kappaB and the Hedgehog Signaling Pathway

Cited by 22 publications

References 31 publications

Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis

Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis

Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

Glycosidic flavonoids and their potential applications in cancer research: a review

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