The effects of ovine growth hormone on protein turnover in rainbow trout

Foster, A. R.; Houlihan, D.F.; Gray, Colin; Médale, Françoise; Fauconneau, Benoı̂t; Kaushikj, S.J.; Bail, P.‐Y. Le

doi:10.1016/0016-6480(91)90302-m

Cited by 83 publications

(22 citation statements)

References 33 publications

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“…Fish respond to stress catabolically, utilising metabolic energy for numerous activities which together act to reduce growth rate. The administration of exogenous GH has been demonstrated to enhance growth in several fish species, including Oncorhynchus mykiss, O. kisutch, O. tshawytscha and Oreochromis mossambicus (Foster et al 1991, Flik et al 1993, McLean et al 1997. GH-induced growth enhancement suggests a reallocation of metabolic energy to anabolic processes and may be indicative of reduced stress as fish subjected to chronic stressors often exhibit compromised growth (Wendelaar Bonga 1997).…”

Section: Discussionmentioning

confidence: 99%

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Deane

Kelly²,

Lo³

et al. 1999

Journal of Endocrinology

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Heat shock protein 70 (HSP70) expression was assessed in hepatic tissue of a marine teleost Sparus sarba after exogenous hormone administration. Using a PCR-amplified, homologous HSP70 cDNA clone, as a probe in Northern analysis, we detected a 2·3 kb transcript which was elevated after exposure to a temperature 7 C above the ambient. For our studies on hormonal effects on HSP70 expression, groups of fish were administered recombinant bream GH (rbGH), ovine prolactin (oPRL) or cortisol daily over a 7-day period. Quantification of hepatic HSP70 transcript revealed that the administration of GH and PRL significantly reduced HSP70 mRNA abundance by 42% and 54% from saline-injected fish respectively. Also hepatic HSP70 levels were reduced by 76% and 64% as determined by immunoblotting after rbGH and oPRL treatment respectively. The administration of exogenous cortisol did not alter hepatic HSP70 mRNA or protein levels in S. sarba. The results obtained in this study are the first evidence for hormonal modulation of heat shock protein expression in fish. The significance of these results is discussed within the context of current knowledge on the roles of these hormones in teleostean stress response.

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Section: Discussionmentioning

confidence: 99%

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Deane

Kelly²,

Lo³

et al. 1999

Journal of Endocrinology

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“…We have identified high-affinity, low-capacity binding sites for GH in tilapia liver. tPRL 177 binds to these sites, albeit with lower affinity than tGH, but equally as well as ovine GH, the actions of which on growth in teleosts have been described (12,47,48 (45,50). This suggests that the actions of tPRL 177 may overlap with those of tPRL 188 and tGH through its ability to bind to their receptors.…”

Section: Discussionmentioning

confidence: 99%

Somatotropic actions of the homologous growth hormone and prolactins in the euryhaline teleost, the tilapia, Oreochromis mossambicus

Shepherd

Sakamoto

Nishioka

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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It is increasingly clear that growth hormone (GH) has growth-promoting effects in fishes, which are mediated in part by the insulin-like growth factor (IGF)-I. Growth-promoting actions of prolactin (PRL) have been reported in higher vertebrates, but are less well established in teleosts. We examined the effects of injecting homologous GH or the two homologous tilapia PRLs (tPRL 177 and tPRL 188 ) on the in vitro incorporation of [ 35 S]sulfate (extracellular matrix synthesis) and [ 3 H]thymidine (DNA synthesis) by ceratobranchial cartilage explants and on IGF-I mRNA levels in tilapia liver. Tilapia GH (tGH) and tPRL 177 stimulated sulfate uptake at the highest doses examined. Thymidine incorporation was stimulated by tPRL 177 . tPRL 188 was without these effects. Consistent with its somatotropic actions, tGH elevated IGF-I mRNA levels in the liver. tPRL 177 also elevated liver IGF-I levels. Consistent with the previously described osmoregulatory actions of GH and PRL in teleosts, we observed that tGH elevated and tPRL 177 and tPRL 188 lowered levels of gill Na ؉ ,K ؉ -ATPase activity. High-affinity, low-capacity binding sites for tGH in the tilapia liver were identified. tPRL 177 binds with lower affinity than tGH to these sites but can displace 125 I-labeled tGH from its receptor. The ability of tPRL 177 to displace tGH was similar to that of ovine GH. tPRL 188 did not displace 125 I-labeled tGH binding. Collectively, this work suggests that tPRL 177 may possess somatotropic actions similar to tGH, but only in freshwater tilapia where tPRL 177 levels are sufficiently high for it to act as a competitive ligand for GH receptors.

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“…Seperti yang dilaporkan oleh beberapa peneliti bahwa dengan pemberian rGH dapat meningkatkan sintesis protein dan menurunkan sintesis lemak pada mamalia (Pell et al, 1990;Johnsson et al, 1987) dan menstimulasi anabolisme dalam meningkatkan penggantian dan sintesis protein pada ikan, yang terjadi pada hati dan otot dengan menstimulasi efisiensi dari translasi ribosom melalui peningkatan konsentrasi mRNA dan ribosom (Foster et al, 1991;Herbert et al, 2001). Mekanisme tersebut diduga melalui optimasi pemanfaatan protein sebagai sumber energi untuk pertumbuhan.…”

Section: Description: the Crude Fiber Not Detectedunclassified

Respons Pertumbuhan Dan Keragaan Darah Dari Tiga Strain Ikan Nila Terhadap Pemberian Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang

Listiyowati

Alimuddin

Sukenda

et al. 2015

Jurnal Riset Akuakultur

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ABSTRAKBerbagai strain ikan nila sudah berhasil dibudidayakan di Indonesia. Penelitian ini dilakukan untuk mengevaluasi penambahan hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH) dalam pakan terhadap performa pertumbuhan dan imunitas tiga strain ikan nila terhadap infeksi bakteri Streptococcus agalactiae. Tiga strain nila yang digunakan adalah strain nila biru (hasil pemuliaan di Sukamandi), srikandi (toleran salinitas tinggi dari Sukamandi) dan nirwana (hasil pemuliaan di Wanayasa)dengan kisaran rerata bobot 0,24-0,4 g/ekor dan panjang standar 1,9-2,27 cm/ekor. Ikan dipelihara dalam bak fiber bulat kapasitas 1,5 ton air sebanyak sembilan buah selama 50 hari, dengan padat tebar 200 ekor/bak. Pakan mengandung rElGH dosis 2 mg/kg pakan diberikan dua hari sekali, selama dua minggu dengan frekuensi tiga kali sehari (pagi, siang, dan sore) secara at satiation (sampai kenyang). Hasil penelitian menunjukkan ikan yang diberi rElGH memiliki pertumbuhan yang lebih baik dibandingkan kontrol, kecuali nila biru. Pertumbuhan terbaik terdapat pada nila srikandi dengan bobot 6,08 ± 1,26 g; laju pertumbuhan harian 0,12 ± 0,03 g/hari dan panjang standar 5,5 ± 0,63 cm. Ikan nila yang mendapatkan perlakuan rElGH pada pakan menunjukkan ketahanan tubuh yang berbeda ketika diuji tantang dengan bakteri S. agalactiae. Ikan nila nirwana memiliki jumlah kematian yang paling sedikit (P<0,05) dibandingkan dengan ikan nila srikandi dan nila biru terhadap infeksi S. agalactiae. Nilai MTD ikan nila nirwana perlakuan rElGH dan kontrol adalah 174,3 jam dan 217,7 jam. Hasil pengamatan uji tantang menunjukkan bahwa tidak ada yang berbeda dalam parameter hematologi. Sebagai kesimpulan, strain ikan nila sangat memengaruhi efektivitas pemberian rElGH dalam memacu pertumbuhan dan imunitasnya. KATAKUNCI: pertumbuhan, imunitas, hormon pertumbuhan rekombinan ikan kerapu kertang (rElGH), Streptococcus agalactiae, ikan nila ABSTRACT:Growth and haematology performance response of three tilapia strains fed on diet containing a recombinant giant grouper growth hormone. By: Nunuk Listiyowati, Alimuddin, Sukenda, and Priadi Setyawan

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The effects of ovine growth hormone on protein turnover in rainbow trout

Cited by 83 publications

References 33 publications

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Somatotropic actions of the homologous growth hormone and prolactins in the euryhaline teleost, the tilapia, Oreochromis mossambicus

Respons Pertumbuhan Dan Keragaan Darah Dari Tiga Strain Ikan Nila Terhadap Pemberian Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang

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