The effects of oxygenation aeration treatment on dissipation behaviors of tricaine mesylate in carp ( Cyprinus carpio ) muscle and water

Qin, Yuhong; Zhang, Jingru; Yuang, Zhang; Li, Fangbing; Li, Yanjie; Yang, Han; Zou, Nan; Pan, Canping

doi:10.1016/j.foodcont.2017.01.027

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…27 In carp, oxygenation aeration treatment reportedly increased the dissipation rate of MS-222. 28 Species-specific differences have been detected regarding MS-222 aversion: medaka avoided MS-222, but carp and rainbow trout did not. 29 These results should be taken into consideration when selecting an anaesthetic agent.…”

Section: Ms-222mentioning

confidence: 99%

Anaesthetics and analgesics used in adult fish for research: A review

Martins

Valentim

Pereira³

et al. 2018

Lab Anim

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The number of fish used in research has increased in the last decades. Anaesthesia is required when fish must be held immobile and it is crucial to promote fish welfare, because these vertebrates can show signs of stress and/or pain during handling, transport, tagging, sampling and invasive procedures. The use of an inadequate anaesthetic protocol can compromise not only the welfare of the fish, but also the reliability of the research results. Thus, the development of suitable anaesthetic regimes for each fish species is important. This article reviews the main anaesthetic and analgesic agents used in adult fish in a research setting.

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Section: Ms-222mentioning

confidence: 99%

Anaesthetics and analgesics used in adult fish for research: A review

Martins

Valentim

Pereira³

et al. 2018

Lab Anim

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“…Pan's group developed a modified multiplug filtration cleanup method for the rapid determination of tricaine residues in fish samples by coupling pulse glow discharge and ion mobility spectrometry with recoveries of 81.2–99.1% (Zou et al, ). Recently, a modified QuEChERS method combined with n ‐hexane delipidation and multiplug filtration cleanup was further developed by Pan's group for the analysis of tricaine in carp muscle by HPLC–MS/MS with recoveries of 85–95% (Qin et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…However, the reported methods use only a structural analog (benzocaine) as an internal standard (Scherpenisse & Bergwerff, 2007) or the external standard method (Li et al, 2014;Qin et al, 2017;Zou et al, 2016) to correct the matrix effects (MEs). MEs have serious impacts on the accuracies and precisions of the developed methods (Chen, Guo, Wang, & Qiu, 2008).…”

mentioning

confidence: 99%

Stable isotope dilution assay for the accurate determination of tricaine in fish samples by HPLC–MS–MS

Xie

Han

et al. 2019

Biomedical Chromatography

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Tricaine methanesulfonate is one of most commonly used anesthetics in fish during blood sampling, artificial propagation and long‐distance transportation. In this study, an accurate method for the quantitative determination of tricaine in fish samples by a stable isotope dilution assay coupled with high‐performance liquid chromatography–triple quadrupole mass spectrometry was developed. Tricaine‐D5 was synthesized and used as an isotopically labeled internal standard for the determination of tricaine. The analytical performance of the method was validated for tricaine determination in marine fish and freshwater fish. The determination of tricaine was linear in the range of 2.0–200.0 μg L−1. The limit of detection and limit of quantitation for fish muscle tissues were 1.0 and 4.0 μg kg−1, respectively. Good recoveries were obtained in the range of 92.08–97.50%. The inter‐ and intra‐assay relative standard deviations (RSD values) were investigated, and the values were 0.39–3.01 and 0.85–2.77%, respectively. The values of CCα and CCβ were 10.21–10.43 and 10.42–10.87 μg kg−1, respectively. The clearance of MS‐222 from grass carp was further studied using our method. The results demonstrate that MS‐222 could be well absorbed and rapidly eliminated after bath administration.

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