The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats

Abstract

The purpose of our studies is to assist in fully understanding the role of oxytocin in the live metabolism by examining the effects of oxytocin, oxytocin receptor antagonist atosiban, and oxytocinatosiban chemicals injected to the rats on activities of carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes in the lung tissue of the rats. For this purpose, 4 different groups each of which contains 6 rats formed. (Control group, oxytocin administered group, atosiban administered group, and both oxytocin and atosiban administered group). The rats were necropsied after an hour from the injection of chemicals into the rats intraperitoneally. The lung tissues of the rats were extracted. Carbonic anhydrase and acetylcholinesterase enzymes activities were measured for the tissues. All the experimental results were provided as mean ± SD (mean standard deviation). Because of the data was compatible with the normal range, it was tested via variance analysis (ANOVA) and then, significance was determined by the Tukey test. Statistical significance was identified to be p <0.05. Accordingly, it was observed that there was neither a decrease of enzyme activities in oxytocin and atosiban administered groups, nor a significant change of enzyme activities in both oxytocin and atosiban administered group. Also, we observed that oxytocin reduced carbonic anhydrase enzyme activity as a systemic effect. We think that this result may contribute to remedy of lung related diseases progressing with oxygen and carbon dioxide transport disorders.

Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles

“…The cytosolic forms of carbonic anhydrase enzymes are hCA I and hCA II. hCA I and hCA II isozymes play a role in respiration and acid–base homeostasis, 6–11 pH and bicarbonate homeostasis, many physiological processes including respiration, bone metabolism, and tumor formation. It is involved in the carbon dioxide hydration catalysis of CAs 12–15 …”

“…CA (carbonate hydrolase, EC 4.2.1.1) is a metalloenzyme that functions in numerous tissues and acts as a catalyst in the reversible hydration of carbon dioxide to bicarbonate. It contains zinc (Zn 2+ ) ions in its active site 6–14 . Carbonic anhydrases are classified into four categories based on where they are found in the body, with at least 16 distinct isoforms ranging from CA I to CA XVI.…”

“…It contains zinc (Zn 2+ ) ions in its active site. [6][7][8][9][10][11][12][13][14] Carbonic anhydrases are classified into four categories based on where they are found in the body, with at least 16 distinct isoforms ranging from CA I to CA XVI. CA isoenzyme inhibitors (hCA I and hCA II) are also employed as active ingredients in pain medications and diuretics to treat cancer, epilepsy, osteoporosis, and other diseases, hypertension, especially hCA II inhibitors, and eye diseases.…”

Oxytocin maintains lung histological and functional integrity to confer protection in heat stroke

“…The present study demonstrated that OT binding to OT receptors has a protective effect against heat-induced ALI by inhibiting the ischemic, oxidative and inflammatory responses in a rat model. The mechanism might involve TLR4/NLRP3/NF-κB, Bax/p53, Bcl-2 40,41 , type A gamma-aminobutyric acid receptor 28 , calpain-1 7 , and carbonic anhydrase and acetylcholinesterase enzymes 42 .…”

Synthesis, characterization, and biological studies of chalcone derivatives containing Schiff bases: Synthetic derivatives for the treatment of epilepsy and Alzheimer's disease

“…Also, for K is , three different concentrations of the compounds ( 3a–j and 5a–f ) were used. [ 41 ] Then, Lineweaver–Burk graphs were drawn according to these measurements. K i values of chalcone derivatives containing Schiff bases ( 3a–j and 5a–f ) were determined from Lineweaver–Burk graphs, as previously described.…”