The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

Abstract

Paeoniflorin (PAE) is the most abundant compound in Xuebijing injection widely used to treat sepsis. We aimed to investigate effect of PAE on expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in a rat model of sepsis. Wistar rats were divided into Normal, Model, and PAE groups (n=20 each). Endotoxin was administrated at 5 mg/ml/kg in Model and PAE rats to establish rat sepsis model. 1 h after endotoxin administration, PAE was administrated at 4 ml/kg in PAE group once per day for 3 days. Routine blood tests and biochemical indexes were assessed, including aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB). The plasma sTREM-1 level was measured using quantitative ELISA. At the end of experiment, the small intestine, liver, kidney and lung were subjected to pathological examinations. A rat model of sepsis-induced multiple organ dysfunction syndrome (MODS) was established successfully with endotoxin administration (5 mg/ml/kg), evidenced by histo-pathological examinations, routine blood tests and biochemical indexes: platelet count decreased and white blood cell count increased (p<0.05), CK-MB and AST increased (p<0.05). PAE treatment significantly reduced the plasma levels of AST, CK-MB, and sTREM-1, compared to Model group (p<0.05). Meanwhile, sepsis-induced damages in the liver, lung, stomach and intestinal mucosa were also markedly ameliorated by PAE treatment. PAE demonstrated a significantly protective effect in a rat model of sepsis by decreasing plasma sTREM-1 level, reducing inflammation, preventing MODS and protecting organ functions.

Paeoniflorin and Hydroxysafflor Yellow A in Xuebijing Injection Attenuate Sepsis-Induced Cardiac Dysfunction and Inhibit Proinflammatory Cytokine Production

“…No significant differences were observed between C0127 and C0127-2 group, suggesting paeoniflorin and HSYA are the core compounds in XBJ for cardiac protection. Two previous studies suggested paeoniflorin rendered cardiac protection in LPS-induced endotoxemia models (Zhai and Guo, 2016b;Liu et al, 2016). Liu et al found 12 mg/kg paeoniflorin once/day reduced plasma CK-MB (a biomarker of severe heart damage) to normal level 72 h after the LPS administration (5 mg/kg) in a rat model (Liu et al, 2016).…”

“…A series of studies indicated these two compounds are promising Xuebijing constituents of therapeutic importance (Cao et al, 2011;Zhai and Guo, 2016b;Cheng et al, 2016;Zou et al, 2018;Shang et al, 2019). Paeoniflorin improved survival and cardiac function in LPS-treated rodents (Cao et al, 2011;Zhai and Guo, 2016b;Liu et al, 2016). HSYA improved ischaemiainduced cardiac haemodynamics by stimulating nucleolinmediated angiogenesis (Zou et al, 2018).…”

Effects of Carnosine (Beta-Alanyl-L-Histidine) in an Experimental Rat Model of Acute Kidney Injury Due to Septic Shock

“…Sepsis is found to be associated with the development of acute kidney injury (AKI) [ 2 ]. Bacterial infections, most commonly due to Gram-negative bacteria and their endotoxins, are the most common underlying cause of sepsis [ 1 – 3 ]. Inflammation associated with sepsis involves a dynamic response of vascularized tissue to injury [ 3 ].…”

“…Bacterial infections, most commonly due to Gram-negative bacteria and their endotoxins, are the most common underlying cause of sepsis [ 1 – 3 ]. Inflammation associated with sepsis involves a dynamic response of vascularized tissue to injury [ 3 ]. In the inflamed tissues, changes in blood flow, vascular permeability, leukocyte accumulation, and edema occur, with a variety of mediators being involved in this chain of events [ 3 ].…”

“…Inflammation associated with sepsis involves a dynamic response of vascularized tissue to injury [ 3 ]. In the inflamed tissues, changes in blood flow, vascular permeability, leukocyte accumulation, and edema occur, with a variety of mediators being involved in this chain of events [ 3 ].…”