The Effects of Photobiomodulation Delivered by Light-Emitting Diode on Stem Cells from Human Exfoliated Deciduous Teeth: A Study on the Relevance to Pluripotent Stem Cell Viability and Proliferation

Vale, Katia Llanos do; Maria, Durvanei Augusto; Picoli, Lara Cristina; Deana, Alessandro Melo; Mascaro, Marcelo Betti; Mesquita‐Ferrari, Raquel Agnelli; Bussadori, Sandra Kalil; Fernandes, Kristianne Porta Santos

doi:10.1089/pho.2017.4279

Cited by 14 publications

(7 citation statements)

References 39 publications

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“…All the studies analyzed showed qualitative and quantitative different parameters and for this reason it is very difficult to compare them and to identify a univocal protocol. Nearly all papers showed that LLLT had a positive effect on HPDLSCs, hDPSCs, hBMSCs and hADSCs proliferation with doses used between 0.5 and 10 J/cm 2 , while doses higher than 10 J/cm 2 exert or no effects [7] or seems to be antiproliferative [93] and similar outcomes were found for LED [104]. In addition, no study showed deleterious effects of LLLT on these cells [105].…”

Section: Discussionmentioning

confidence: 85%

Analysis of Changes Induced In Human Periodontal Ligament, Dental Pulp, Bone Marrow and Adipose Stem Cells by Low Level Laser Therapy: A Review and New Perspectives

Grassia

Vitale

d’Apuzzo

et al. 2018

BJSTR

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The biomodulation and the biostimulation of Low Level Laser Therapy (LLLT) promote wound healing, dentine repairing processes and stimulate the biological activity of human Periodontal Ligament Stem Cells (HPDLSCs), human Dental Pulp Stem Cells (HDPSCs), human Bone Marrow Stem Cells (HBMSCs) and human Adipose Stem Cells (HADSCs). The aim of the study was to review the literature on the LLLT effects on HPDLSCs, HDPSCs, HBMSCs and HADSCs. The electronic search was performed collecting articles in the PubMed, Medline, Scopus, Lilacs and Google Scholar databases from January 1990 to January 2018. LLLT was able to significantly increase HPDLSCs, HDPSCs, HBMSCs and HADSCs proliferation rate, stimulating osteogenic differentiation and opening new possibilities in the tissue regeneration. However, the research background on this topic showed high heterogeneity and the in vitro studies cannot always mimic the in vivo conditions. Thus, further clinical studies should be performed to determine the appropriate dose of irradiation in different patients.

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Section: Discussionmentioning

confidence: 85%

Analysis of Changes Induced In Human Periodontal Ligament, Dental Pulp, Bone Marrow and Adipose Stem Cells by Low Level Laser Therapy: A Review and New Perspectives

Grassia

Vitale

d’Apuzzo

et al. 2018

BJSTR

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“…A previous study by Vale et al . (34) found genomic DNA damage when a single LED irradiation of 4 J cm −2 was applied in MSCs. It is worth mentioning that the irradiance used by the researchers was 37 mW cm −2 for 1 min and 47 s, more than twice the irradiance employed in our experimental set and for less than twice the irradiation time.…”

Section: Discussionmentioning

confidence: 99%

Light‐emitting Diode Can Enhance the Metabolism and Paracrine Action of Mesenchymal Stem Cells

Mansano

Rocha

Teixeira

et al. 2023

Photochem & Photobiology

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This study investigated the influence of red light‐emitting diodes (LED, 630 nm) on different irradiation parameters and the number of applications on mesenchymal stem cells derived from adipose tissue (AdMSCs) metabolism and paracrine factors. The AdMSCs were irradiated with a LEDbox device (output power: 2452.5 mW; laser beam: 163.5 cm2; irradiance: 15 mW cm−2) using radiant exposures of 0.5, 2, and 4 J cm−2, respectively. AdMSCs were irradiated once or every 48 h up to three irradiations. All molecular analyses were performed 24 h after the last irradiation. LED did not induce changes in cell count, DNA damage, and oxidative stress. A significant repercussion of the LED has been noticed after three irradiations with 4 J cm−2. AdMSCs had higher levels of IL‐6, IGF‐1, and NOx index. A higher ATP content and MMT/Resazurin assay were identified in AdMSCs irradiated three times with 4 J cm−2. Mitochondrial basal respiration, maximal respiration and proton leak under metabolic stress were reduced by 0.5 and 2 J cm−2 irradiations. These data showed that three LED irradiations with 4 J cm−2 may be a suitable parameter for future AdMSCs therapy because of its improved metabolic activity, ATP content, and IL‐6, IGF‐1, and nitric oxide secretion.

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“…Tani et al [44] reported that proliferation and osteogenic differentiation is increased at 635 nm of wavelength in in vitro study. Not only other studies focus on the osteogenetic effects of PBM [45][46][47][48][49], but also Peat et al [50] reported that PBM therapy using neodymium-doped yttrium aluminum garnet laser at 1,064 nm can enhance the angiogenesis which can promote the bone healing. Furthermore, in osteogenic aspects, LLLT irradiation (gallium-aluminumarsenide diode; λ 830 nm, 30 mW, θ 1 mm) showed almost two-times greater bone formation rate with new bone structural unit in histometric analysis (13.29% vs. 26.17%).…”

Section: Photobiomodulation Therapy For Bone Regenerationmentioning

confidence: 99%

Application of photobiomodulation therapy in the recovery of a fracture model in different countries: a concise review

Kim

Padalhin²

2022

Medical Lasers

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The bone is a dynamic biological tissue composed of metabolically active cells which are integrated into a hard framework. However, it can be injured due to trauma or infections and this can result in bone defects. Hence, bone generation or bone healing has been an interesting field of orthopedics and has been the subject of multiple studies. As a result, various substances which promote osteogenesis and angiogenesis have been identified, but their mechanism of action has not yet been completely elucidated. Owing to its anti-inflammatory and wound-healing effects, photobiomodulation (PBM) has been proposed in the treatment of various disease conditions. Specifically, in orthopedics, multiple studies have been conducted to treat bone generation or bone union using PBM. The clinical improvement of bone healing as well as various substances and mechanisms of osteogenesis and angiogenesis by PBM have been identified although the mechanism of action is not fully understood. This review summarizes the published reports on the bone healing effects of PBM treatment in fracture sites.

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The Effects of Photobiomodulation Delivered by Light-Emitting Diode on Stem Cells from Human Exfoliated Deciduous Teeth: A Study on the Relevance to Pluripotent Stem Cell Viability and Proliferation

Abstract: LED irradiation at 630 nm (37 mW/cm, 75 mW) with radiant exposure of 16 J/cm was capable of inducing a proliferative response in stem cells from the pulp tissue of deciduous teeth without affecting mitochondrial function or inducing senescence.

Cited by 14 publications

References 39 publications

Analysis of Changes Induced In Human Periodontal Ligament, Dental Pulp, Bone Marrow and Adipose Stem Cells by Low Level Laser Therapy: A Review and New Perspectives

Analysis of Changes Induced In Human Periodontal Ligament, Dental Pulp, Bone Marrow and Adipose Stem Cells by Low Level Laser Therapy: A Review and New Perspectives

Light‐emitting Diode Can Enhance the Metabolism and Paracrine Action of Mesenchymal Stem Cells

Application of photobiomodulation therapy in the recovery of a fracture model in different countries: a concise review

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