The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis

Silva, Zenildo Santos; França, Cristiane Miranda; Prates, Renato Araújo; Botta, Sérgio Brossi; Mesquita‐Ferrari, Raquel Agnelli; Ana, Patrícia Aparecida; Pavani, Christiane; Fernandes, Kristianne Porta Santos; Silva, Daniela de Fátima Teixeira da; Hamblin, Michael R.; Bussadori, Sandra Kalil

doi:10.1007/s10103-019-02857-7

Cited by 9 publications

(16 citation statements)

References 31 publications

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“…Studies have shown that the isolated action of aPDT is not completely efficient for the microbial reduction and painful symptoms of patients [19], highlighting the importance of dental professionals in both the treatment and prevention of deep caries-affected dentin of permanent teeth [20]. Procedures using aPDT have also been developed to the treatment of dental caries [3,4,20], however, there are few controlled clinical trials in the literature confirming its efficacy. Papacarie™ is a gel composed of papain and chloramine [8,9] employed for the partial removal of carious tissue, effective against bacteria due to its properties, but some studies show that the antibacterial action is not so evident.…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial photodynamic therapy (aPDT) is a promising new approach that aims to promote the reduction of microorganisms and can be an innovative treatment to control oral biofilm microorganisms [1,2]. Furthermore, it has been used in the treatment of dental caries [3]. Many studies have been conducted to confirm the effectiveness of this treatment modality [3][4][5][6].…”

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

“…Alternatively, chemo-mechanical caries removal agents can be used instead [8]. Papacarie ™ is considered a minimally invasive clinical treatment approach to treating dental caries with proven efficacy [3,[7][8][9][10]. However, it is necessary to improve its antimicrobial action.…”

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

“…Bixa orellana, popularly known as "urucum", is a plant native to Brazil. This dye has important antioxidant and antimicrobial activities, and recent studies have shown its potential as a photosensitizer in aPDT [3,[11][12][13]. Due to its positive characteristics, especially regarding the lack of mutagenic and cytotoxic activity and antimicrobial effect, the Bixa orellana extract could be considered a suitable candidate as a photosensitizer.…”

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

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Antimicrobial photodynamic therapy as an adjunct for management of deep caries lesions - Study protocol for a randomized, controlled clinical trial

Martins¹,

Sena²,

Paula³

et al. 2022

Preprint

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Background: Alternatively to conventional treatments, chemo-mechanical caries removal agents can be used. A modality of treatment that has been increasing in dentistry is antimicrobial photodynamic therapy (aPDT). Bixa orellana, is being researched for application in aPDT. This protocol aims to determine the effectiveness of aPDT with Bixa orellana extract in deep caries lesions.Methods: A total of 160 teeth with deep occlusal dental caries will be selected and divided in 3 groups: G1 - control group (Caries removal with a lowspeed drill); G2 - Partial Caries Removal with Papacarie; G3 - Partial Caries Removal with Papacarie and application Bixa orellana extract (20%); G4 - Partial Caries Removal with Papacarie and application Bixa orellana extract (20%) with LED (aPDT). After treatment, all the teeth will be restored with glass ionomer cement and followed up clinically and radiographically, with evaluations at immediately, 1 week, 1, 3, 6, and 12 months. Dentin samples before and after treatment will be analyzed microbiologically. The data will be submitted to descriptive statistical analysis of the association between the categorical variables using the chi-square test and Fisher exact text. The Student t test and analysis of variance will be used for the comparison of mean signs and symptoms of reversible pulpitis. Pearson correlation coefficients will be calculated for the analysis of correlations among the continuous variables.Discussion: Procedures using aPDT have been developed for the treatment of dental caries, but, there are few controlled clinical trials in the literature confirming its efficacy.Trial registration: This protocol is registered at ClinicalTrials.gov under the number NCT05236205 and it was first posted on 01/21/2022 and last updated on 05/10/2022, https://clinicaltrials.gov/ct2/show/NCT05236205?term=NCT05236205&draw=2&rank=1.

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Section: Discussionmentioning

confidence: 99%

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

Section: Introduction Background and Rationale {6a}mentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial photodynamic therapy as an adjunct for management of deep caries lesions - Study protocol for a randomized, controlled clinical trial

Martins¹,

Sena²,

Paula³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Papain promotes proteolysis of the collagen fibrils exposed in the decayed tissue, which further softens the decayed tissue, facilitating its removal with hand instruments [16] . After use, the gel often changes color and becomes cloudy or produces bubbles, making it easier to identify the reaction, completion or absence (i.e., no decomposed tissue remains) [25] . The softened tissue is then removed with non-cutting tip instruments.…”

Section: Papacariementioning

confidence: 99%

Minimally invasive treatment of dental caries, an update

Ornelas¹,

Longoria²,

Silva³

et al. 2022

Int. J. Appl. Dent. Sci.

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The importance of this research lies in the effectiveness of the different minimally invasive techniques for the management of carious lesions. Objective: To analyze the minimally invasive treatment for the removal of carious lesions. The techniques evaluated were: conventional method, ART, papacarie, laser and abrasive air. Methodology: A compilation of articles published in the last 5 years was carried out using the PubMed electronic database. Abstracts and full texts were identified that included information on the techniques for minimally invasive treatment of carious lesions: the conventional method, ART, papacarie, laser and abrasive air. Results: The conventional technique is the most commonly used at present, but it is the most aggressive because it removes both healthy and infected tissue. ART is one of the most economical and least painful measures for caries management. Papacarie is a minimally invasive chemical removal method and does not require local anesthesia. Laser for caries removal is expensive, but it is one of the least abrasive ways to manage it, and the abrasive air does not irritate pulp or adjacent tissues. Conclusions: Removal of dental caries with rotary instruments is frequently associated with thermal and pressure effects on the pulp, resulting in pain. Due to the shortcomings of the bur, alternatives such as chemo-mechanical caries removal with sharp instruments (ART), laser and abrasive air were developed, which have a disintegrating effect on the caries tissue, while leaving healthy dentin largely intact.

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Photodynamic therapy with acai (Euterpe oleracea) and blue light in oral cells: A spectroscopic and cytotoxicity analysis

Silva

Andreo

Gonçalves

et al. 2022

Journal of Biophotonics

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Objective To evaluate the potential of photodynamic therapy (PDT) with blue light‐emitting diode (LED) 460 nm at 25, 50 and 100 J/cm2 using three concentrations of acai extracts (100, 40, and 10 mg/ml), in the proliferation and viability of head and neck tumor lines (SCC9). Methods Three groups of cells were analyzed for 3 days in an in vitro assay with MTT (3‐ (4,5‐dimethylthiazol‐2‐yl) ‐2,5, ‐diphenyltetrazolium bromide) and crystal violet: cells in the absence of acai extract and PDT (control group); cells in the presence of acai extract and no light; and cells in the presence of acai extract and LED blue light (PDT groups). Results When using acai as a PS combined with blue LED (460 nm, 0.7466 cm2, 1000 mW/cm2) and irradiation at 25, 50, and 100 J/cm2, after 72 h, cell viability (p < 0.0001 vs. control, p = 0.0027 vs. 100 mg/ml açai group, p = 0.0039 vs. 40 mg/ml açai group, p = 0.0135 vs. 10 mg/ml açai group; One‐Way ANOVA/Tukey) and proliferation (p < 0.05, One‐Way ANOVA/Tukey) decreased. Conclusion The acai in question is a potential photosensitizer (PS), with blue light absorbance and efficacy against head and neck tumor lines (SCC9).

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The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis

Cited by 9 publications

References 31 publications

Antimicrobial photodynamic therapy as an adjunct for management of deep caries lesions - Study protocol for a randomized, controlled clinical trial

Antimicrobial photodynamic therapy as an adjunct for management of deep caries lesions - Study protocol for a randomized, controlled clinical trial

Minimally invasive treatment of dental caries, an update

Photodynamic therapy with acai (Euterpe oleracea) and blue light in oral cells: A spectroscopic and cytotoxicity analysis

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