The effects of photodynamic treatment with new methylene blue N on the Candida albicans proteome

Abstract

Photodynamic treatment induces fluence-dependent protein damage and modifies C. albicans proteome.

Singlet oxygen production by a polypyridine ruthenium (II) complex with a perylene monoimide derivative: A strategy for photodynamic inactivation of Candida albicans

“…This might include generation of singlet oxygen, and subsequent pollutant degradation and photodynamic activity [7,10,15]. A number of perylene-3,4,9,10-tetracarboxylic acid (diimide) derivatives, known as PDIs, and polypyridine complexes derived from perylenes have shown good results in the production of reactive species of reactive oxygen species (ROS) [11,[16][17][18]. The ligands containing the perylene moiety are reddish dyes with very high quantum yields of luminescence.…”

“…The perylene dyes are known for their poor solubility in organic solvents (typically 1-2 mg L À1 ) [11,21,22]. Their PDI diimide derivatives with symmetrical and unsymmetrical secondary, or tertiary alkyl side chains N,N'-bis(1-isobutyl-3 methylbutyl) perylene-3,4:9,10-bis(dicarboximide), N,N'-bis(1-butylhexyl)perylene-3,4:9,10-bis(dicarboximide) and N,N'-bis(5-tert-butyl-1,3,4-thiadiazole-2-yl)perylene-3,4:9, 10-bis(dicarboximide) were synthesized and had significantly improved solubility (10 mg/mL) in dichloromethane, chloroform, ether, hexane, chlorobenzene and toluene [16,17]. Single or double amine substitution on the perylene core can also lead to a red shift of the absorption maximum up to 750 nm [11,22,23].…”

“…The shape of the fluorescence spectra of cis-[Ru(phen) 2 (pPDI)] 2 + is characteristic of the free perylene emission [10,11,13,16,22,43].…”

The second International Symposium on Fungal Stress: ISFUS

“…Gilberto U. L. Braga explained that antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional antifungal agents that can be used to kill fungi, which cause diseases in animals or plants (de Menezes et al, 2014;Gonzales et al, 2017). APDT, using phenothiazinium photosensitizers, efficiently kills planktonic cells of Candida species and conidia of several pathogenic fungi, damages the fungal plasma membrane increasing its permeability and greatly impacting their proteomes (Brancini et al, 2016).…”

Chemical features of the photosensitizers new methylene blue N and S137 influence their subcellular localization and photoinactivation efficiency in Candida albicans

“…Conversely, energy transfer or Type II reaction results in the formation of singlet oxygen. In either case, reactive oxygen species (ROS) such as singlet oxygen, superoxide radical anions, and hydroxyl radicals have a broad spectrum of activity and can damage several microbial targets such asas among the various proteins, lipids, and nucleic acids encountered, therefore making selection of resistant strains unlikely (Brancini et al 2016;Wainwright et al 2017). Among photoantimicrobials evaluated as antifungals, the phenothiazinium dyes methylene blue and toluidine blue are the most commonly used, mainly due to their low toxicity and their long-established use for other clinical applications (Rodrigues et al 2013;Wainwright et al 2017).…”