The Effects of Polyamines and Hydrogen Peroxide on Root Formation in Olive and the Role of Polyamines as an Early Marker for Rooting Ability

Rugini, Eddo; Francesco, Giuseppe; Muganu, Massimo; Astolfi, Stefania; Caricato, G.

doi:10.1007/978-1-4615-5403-5_10

Cited by 28 publications

(21 citation statements)

References 13 publications

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“…There are a few studies on the correlation between H 2 O 2 and plant rooting. In olive cuttings, H 2 O 2 promotes early rooting (Rugini et al 1997). Arabidopsis thaliana rhd 2 (root hair defective 2) mutants have marked reductions in reactive oxygen species, and H 2 O 2 is involved in the formation of root hairs (Foreman et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Development of Lateral Roots in Soybean

Guo-xing

Zhang

Youliang

2006

JIPB

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In order to determine whether hydrogen peroxide (H 2 O 2 ) generated by polyamine oxidative degradation is involved in the development of lateral roots in soybean, the length and the number of lateral roots, the activities of polyamine oxidases and diamine oxidases, and the endogenous free polyamine and H 2 O 2 content were analyzed in soybean (Glycine max (Linn.) Merr.) main roots of 2-d-old seedlings after treatments for 2 d with exogenous β β β β β-hydroxyethylhydrazine (an inhibitor of polyamine oxidases), H 2 O 2 , putrescine, cyclohexylamine (an inhibitor of spermidine synthase) or N,N'-dimethylthiourea (a scavenger of hydrogen peroxide). β β β β β-hydroxyethylhydrazine treatment strongly inhibited the development of lateral roots in soybean seedlings, reduced the activities of polyamine oxidases and diamine oxidases, decreased H 2 O 2 levels, and led to the accumulation of endogenous polyamines in the main roots. The inhibitory effect of β β β β β-hydroxyethylhydrazine on root development could be alleviated by exogenously applied 10 µmol/L H 2 O 2 (a major product of polyamine oxidation). Treatment with cyclohexylamine and putrescine promoted root growth slightly, but treatment with cyclohexylamine plus N,N'-dimethylthiourea or putrescine plus N,N'-dimethylthiourea prevented the development of soybean lateral roots. The effects of these treatments on the development of soybean lateral roots were consistent with the changes in endogenous H 2 O 2 levels. These results suggest that the development of soybean lateral roots is associated with the oxidative degradation of polyamines, and that their products, especially H 2 O 2 , are likely to play an important role in the growth of soybean lateral roots.

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Section: Discussionmentioning

confidence: 99%

Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Development of Lateral Roots in Soybean

Guo-xing

Zhang

Youliang

2006

JIPB

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“…Quantitative and qualitative differences in rooting between genotypes and varying environmental conditions during tree growth could not be equalized by H 2 O 2 application. No interaction between years or cultivars and the treatment was obtained (Rugini et al 1997, Sebastiani and Tognetti 2004). The strong influence of genetic factors on olive rooting efficiencies encourages searching for appropriate molecular markers for tree breeding.…”

Section: Introductionmentioning

confidence: 94%

Physiologic responses and gene diversity indicate olive alternative oxidase as a potential source for markers involved in efficient adventitious root induction

Macedo

Cardoso

Hernández

et al. 2009

Physiologia Plantarum

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Olive (Olea europaea L.) trees are mainly propagated by adventitious rooting of semi-hardwood cuttings. However, efficient commercial propagation of valuable olive tree cultivars or landraces by semi-hardwood cuttings can often be restricted by a low rooting capacity. We hypothesize that root induction is a plant cell reaction linked to oxidative stress and that activity of stress-induced alternative oxidase (AOX) is importantly involved in adventitious rooting. To identify AOX as a source for potential functional marker sequences that may assist tree breeding, genetic variability has to be demonstrated that can affect gene regulation. The paper presents an applied, multidisciplinary research approach demonstrating first indications of an important relationship between AOX activity and differential adventitious rooting in semi-hardwood cuttings. Root induction in the easy-to-root Portuguese cultivar 'Cobrançosa' could be significantly reduced by treatment with salicyl-hydroxamic acid, an inhibitor of AOX activity. On the contrary, treatment with H2O2 or pyruvate, both known to induce AOX activity, increased the degree of rooting. Recently, identification of several O. europaea (Oe) AOX gene sequences has been reported from our group. Here we present for the first time partial sequences of OeAOX2. To search for polymorphisms inside of OeAOX genes, partial OeAOX2 sequences from the cultivars 'Galega vulgar', 'Cobrançosa' and 'Picual' were cloned from genomic DNA and cDNA, including exon, intron and 3'-untranslated regions (3'-UTRs) sequences. The data revealed polymorphic sites in several regions of OeAOX2. The 3'-UTR was the most important source for polymorphisms showing 5.7% of variability. Variability in the exon region accounted 3.4 and 2% in the intron. Further, analysis performed at the cDNA from microshoots of 'Galega vulgar' revealed transcript length variation for the 3'-UTR of OeAOX2 ranging between 76 and 301 bp. The identified polymorphisms and 3'-UTR length variation can be explored in future studies for effects on gene regulation and a potential linkage to olive rooting phenotypes in view of marker-assisted plant selection.

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“…Moreover, treatment of mung bean seedlings with H2O2 resulted in a decrease of POX activity (Li et al, 2009c). In olive, exogenous application of Put induced a POX peak (Özkaya and Celik, 1994;Rugini et al, , 1997) which could be a result of Put degradation through the Δ'-pyrroline pathway, where H2O2 is the main by-product (Tiburcio et al, 1997). H2O2 accumulation could then stimulate POX activity (Gaspar et al, 1997;Rugini et al, 1992) and ultimately root formation.…”

Section: Role Of Hydrogen Peroxidementioning

confidence: 99%

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

Porfírio

Silva

Cabrita

et al. 2016

Scientia Horticulturae

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Olive (Olea europaea) is one of the most important fruit species in the Mediterranean basin, where 95% of the world's olive orchards are planted, and it has become an economically valuable crop worldwide, due to an increasing interest in olive oil for human consumption. New olive orchards are being planted outside the Mediterranean, calling for an effort to identify the genotypes best adapted to the new conditions. However, some olive cultivars remain difficult to propagate, which significantly reduces the capacity to use the full genetic diversity of the species. Improving rooting ability in cuttings from recalcitrant olive cultivars has become a critical topic, which implies fundamental research on the anatomy, physiology, biochemistry and genetics of the adventitious root formation process. Besides, the existence of different rooting behaviors among olive cultivars also makes the species a candidate model plant for these studies. Olive propagation techniques evolved through time from field-or nursery-planted hardwood cuttings, to semi-hardwood cuttings in greenhouses under mist, and, more recently, to in vitro culture techniques. Nevertheless, research about adventitious root formation carried on each propagation method was mostly based on trial and error approaches. Researchers have mainly investigated different factors involved in the process of adventitious rooting by testing their effect in the rooting capacity of different cultivars, leading to a high dispersion and fragmentation of the available information. The goal of this review is to present the most relevant results achieved on adventitious root formation in olive cuttings, aiming to provide an integrated perspective of the current knowledge.

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The Effects of Polyamines and Hydrogen Peroxide on Root Formation in Olive and the Role of Polyamines as an Early Marker for Rooting Ability

Cited by 28 publications

References 13 publications

Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Development of Lateral Roots in Soybean

Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Development of Lateral Roots in Soybean

Physiologic responses and gene diversity indicate olive alternative oxidase as a potential source for markers involved in efficient adventitious root induction

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

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