The Effects of Polypodium leucotomos Extract on the Specific Antibody Production Patterns in BALB/c Mice Immunized with Third Stage Larvae Antigens of Anisakis simplex

Cuéllar, C.; Martínez, María-Aránzazu; Fernández, Felipe; Fernández, Agustín

doi:10.1076/phbi.35.3.153.13292

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…In summary, our results using this helminth model seem to indicate that Anapsos downregulates the Th2 responses generated against T. spiralis antigens. Further investigations are required in order to clarify whether this downregulation results from the direct induction of the opposite Th1 subsets as suggested by Cuellar et al (1997) and indicated here by the increased IgG3 levels, or perhaps this treatment was driving the immune system towards an irrelevant Th2 response induced by thymus independent epitopes present in the plant extract.…”

Section: Discussionmentioning

confidence: 76%

“…It is important to comment on the dynamics of the IgG3 response. Contrary to other helminths (Cuellar et al, 1997) the Trichinella antigens classically induce high levels of IgG3 (Robinson et al, 1994). Class switch to IgG3 is supposed to be regulated by the Th1 cytokine g-IFN (Snapper et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Further approaches to the immunomodulant effects of Anapsos have been attempted in animal models infected by parasites. A marked reduction in IgG1 levels together with an increase of the IgG2a was observed following treatment of mice prior to or after immunization with the crude homogenate antigens from the third stage larvae of the nematode parasite Anisakis simplex, thus suggesting a reverse effect of the anhelmintic Th2 type response towards the Th1 type (Cuellar del Hoyo et al, 1997).…”

Section: Introductionmentioning

confidence: 96%

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Modulation by Anapsos (Polypodium leucotomos extract) of the antibody responses against the nematode parasiteTrichinella spiralis

et al. 1999

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The immunomodulant effects of Anapsos, an extract of the naturally occurring fern Polypodium leucotomos was assessed in Balb/c mice immunized with a crude soluble extract (CSE) of Trichinella spiralis L1 larvae. Treatment from day 10 to 1 prior to immunization caused a significant reduction (p < 0.05) in total antibody levels (IgG + M) that was evident from week 2 onwards. Suppression of the IgG1 response was transient, as serum levels were significantly (p < 0.01) decreased in treated animals during weeks 2 and 3 post‐immunization, afterwards increasing to values similar to those in the control group. An opposite pattern was observed in the IgG2a and IgG 2b profiles where, following a brief increase in weeks 3 and 1, respectively, the values fell below those of the control and remained for the whole observation. Anapsos potentiates the IgG3 response against T. spiralis CSE. Deglycosylation of the CSE used in the ELISA assay significantly reduces the IgG3 recognition capacity in both control and treated mice. This treatment did not affect the time‐course of an intestinal infection by T. spiralis. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Modulation by Anapsos (Polypodium leucotomos extract) of the antibody responses against the nematode parasiteTrichinella spiralis

et al. 1999

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Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD

Martínez-Fernández

Nogal-Ruiz

López‐Abán

et al. 2004

Veterinary Parasitology

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Humoral immune responses induced by Gymnorhynchus gigas extracts in BALB/c mice

Rodero,

Cuéllar

1999

J. Helminthol.

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The aim of this study was to determine if the plerocercoid larvae of Gymnorhynchus gigas, a common cestode of the ray’s bream (Brama raii), possess antigenic compounds potentially capable of provoking anaphylactic episodes. A murine experimental model, using BALB/c mice, was developed to study the humoral immune response induced by G. gigas extracts. A highly specific humoral immune response was detected and cross-reactions were not observed between parasite and host antigens. The presence of IgM and IgG3 levels suggest the presence of thymus-independent antigens in the parasitic extract. The IgG antibody class showed the highest levels, with the IgG1 the predominant subclass. These IgG1 levels are in accordance with the supposed presence of a type I allergic reaction after the ingestion of G. gigas plerocercoids parasitizing fish, as well as inducing anaphylaxia in fish. These results indicate that somatic products released from ingested larvae of G. gigas could induce the development of a Th2 response capable of causing allergic disorders.

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The Effects of Polypodium leucotomos Extract on the Specific Antibody Production Patterns in BALB/c Mice Immunized with Third Stage Larvae Antigens of Anisakis simplex

Cited by 8 publications

References 20 publications

Modulation by Anapsos (Polypodium leucotomos extract) of the antibody responses against the nematode parasiteTrichinella spiralis

Modulation by Anapsos (Polypodium leucotomos extract) of the antibody responses against the nematode parasiteTrichinella spiralis

Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD

Humoral immune responses induced by Gymnorhynchus gigas extracts in BALB/c mice

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