Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD

Martínez-Fernández, A. R.; Nogal-Ruiz, Juan José; López‐Abán, Julio; Ramajo, V.; Oleaga, Ana; Manga-González, Yolanda; Hillyer, George V.; Muro, Antonio

doi:10.1016/j.vetpar.2004.07.023

Cited by 58 publications

(50 citation statements)

References 26 publications

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“…Gerber (11) found higher immune responses in guinea pigs vaccinated against canine parvovirus, in pigs vaccinated against pseudorabies virus, and in cats vaccinated against feline infectious virus when Quil A together with oil was used as an adjuvant than when Quil A or oil was used alone. Martínez-Fernández et al (17) reported an increased immune response induced by Fh12 FABP of Fasciola hepatica in oil emulsion containing Quil A in sheep. Oil emulsion provides the depot effect with progressive release of the antigens at the site of injection.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Xiao

Rajput

Liu

et al. 2007

Clin Vaccine Immunol

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Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals. Vaccination against FMD is a routine practice in many countries where the disease is endemic. This study was designed first to investigate the extract of the seeds of Momordica cochinchinensis (Lour.) Spreng. (ECMS) for its adjuvant effect on vaccination of inactivated FMDV antigens in a guinea pig model and then to evaluate the supplement of ECMS in oil-emulsified FMD vaccines for its immunopotentiation in pigs. The results indicated that ECMS and oil emulsion act synergistically as adjuvants to promote the production of FMDV-and VP1-specific immunoglobulin G (IgG) and subclasses in guinea pigs. A supplement of ECMS in a commercial FMD vaccine significantly enhanced FMDV-specific indirect hemagglutination assay titers as well as VP1-specific IgG and subclasses in pigs. Therefore, ECMS could be an alternative approach to improving swine FMD vaccination when the vaccine is poor to induce an effective immune response.Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals, particularly domestic species, such as cattle, swine, and sheep. Financial losses due to FMD are huge, and it is believed to be the most economically important animal disease in the world (26). Vaccination against FMD is a routine practice in many countries where the disease is endemic. Most commercially available FMD vaccines are inactivated whole-virus preparations containing oil emulsions as an adjuvant to improve their efficacy. However, some FMD vaccines have been reported to induce poor immune responses. For example, Xie et al. (33) observed that only 20.9% of piglets produced immune responses with antibody titers high enough for protection following vaccination against FMD (type O) in the Ningxia province of China. Hao et al. (12) analyzed 91 serum samples of pigs having received vaccination against FMD (type O) and found that only 31.9% of the samples had antibody titers required for immune protection immunity. Therefore, there is a need to improve currently available FMD vaccines in order to effectively protect animals from FMD infection.Our previous research has demonstrated that a supplement of saponin extracted from the bark of Quillaja saponaria Molina (Quil A) in vaccines significantly enhances immune responses to FMD vaccination (32). However, Quil A may be limited in veterinary use because of its hemolytic activity (23). Another study has shown that an extract from a traditional Chinese medicine, the seeds of Momordica cochinchinensis (Lour.) Spreng. (ECMS), has an adjuvant effect on the immune responses elicited by ovalbumin (OVA), with a much lower hemolytic activity (30). ECMS has also been proven effective in enhancing immune responses to vaccination against avian influenza (H5N1) in chickens (24). The medical use of the seeds has been described in ancient Chinese medical literature, Kai Bao Materia Medica, from the Song Dynasty (AD 793) (10) and is currently included in both Chinese pharmacopoei...

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Section: Discussionmentioning

confidence: 99%

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Xiao

Rajput

Liu

et al. 2007

Clin Vaccine Immunol

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“…Proteolytic enzymes are important for digestion of the host tissue and are used in the parasite's defense mechanism. The proteolytic enzymes and antioxidants have been proposed as vaccine candidates against fascioliasis (5)(6)(7)(8)(9)(10)(11)(12)(13).…”

mentioning

confidence: 99%

“…Studies on F. hepatica fatty acid binding proteins (FABPs) have been focused mainly on their potential application as vaccine candidates against fascioliasis (6,10). However, no information is currently available on how FABPs interact with cells of the innate immune system to modulate host immunity.…”

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confidence: 99%

Fasciola hepatica Fatty Acid Binding Protein Induces the Alternative Activation of Human Macrophages

Figueroa-Santiago

Espino

2014

Infect Immun

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The liver fluke Fasciola hepatica is a highly evolved parasite that uses sophisticated mechanisms to evade the host immune response. The immunosuppressive capabilities of the parasite have been associated with antigens secreted through the parasite's tegument, called excretory-secretory products (ESPs). Proteomic studies have identified the fatty acid binding protein (FABP) as one of molecules present in the parasite ESPs. Although FABP has been investigated for potential use in the development of vaccines against fascioliasis, its direct interaction with cells of immune system has not been studied. In this study, FABP was purified in native form from soluble extracts of F. hepatica adult flukes using a combination of molecular sieving chromatography and preparative isoelectric focusing. The immunological effect of the purified protein, termed Fh12, was assayed in vitro using monocyte-derived macrophages (MDM) obtained from healthy human donors. Results from the assay indicate that Fh12 produced a significantly increased arginase expression and activity and induced the expression of chitinase-3-like protein (CHI3L1). The assay also showed that Fh12 downregulated the production of nitric oxide (NO) and the expression of nitric oxide synthase (NOS2). This indicates that Fh12 induced the production of alternatively activated macrophages (AAM). The results also demonstrated the ability of Fh12 to downregulate the secretion of the proinflammatory and inflammatory cytokines tumor necrosis factor alpha (TNF-␣), interleukin-12 (IL-12), and IL-1␤B, even after stimulation with lipopolysaccharide (LPS), as well as its ability to stimulate the overexpression of IL-10. These results suggest a potent anti-inflammatory role for Fh12, which could occur via targeting of Toll-like receptor 4 (TLR4).

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“…The mixture of saponin with oil adjuvant is not new, and the synergistic effects of this combination on immunization have been reported previously. For example, Gerber reported that Quil A, in combination with oil as an adjuvant, promoted greater immune responses in pigs immunized against pseudorabies virus, in guinea pigs immunized against canine parvovirus, and in cats immunized against feline infectious virus (33), Martinez-Fernandez et al found that an oil emulsion containing Quil A increased the immune responses induced by Fh12 fatty acid-binding protein of Fasciola hepatica in sheep (34), and Xiao et al observed that supplements of saponins such as Quil A and extract from the seeds of Momordica cochinchinensis (Lour.) Spreng in FMD vaccines significantly enhanced immune responses in pigs (29,35).…”

Section: Discussionmentioning

confidence: 99%

Rapeseed Oil and Ginseng Saponins Work Synergistically To Enhance Th1 and Th2 Immune Responses Induced by the Foot-and-Mouth Disease Vaccine

Zhang

Wang

et al. 2014

Clin. Vaccine Immunol.

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Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD

Cited by 58 publications

References 26 publications

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Fasciola hepatica Fatty Acid Binding Protein Induces the Alternative Activation of Human Macrophages

Rapeseed Oil and Ginseng Saponins Work Synergistically To Enhance Th1 and Th2 Immune Responses Induced by the Foot-and-Mouth Disease Vaccine

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