The Effects of Pre-Storage Leukoreduction on the Conservation of Bovine Whole Blood in Plastic Bags

Vinholte, Brena Peleja; Sousa, Rejane dos Santos; Assis, Francisco Flávio Vieira de; Neto, Osvaldo Gato Nunes; Portela, Juliana Machado; Pinto, Gilson Andrey Siqueira; Ortolani, Enrico Lippi; Benesi, Fernando José; Júnior, Raimundo Alves Barrêto; Minervino, Antônio Humberto Hamad

doi:10.3390/biology9120444

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…However, the leukoreduction process removes most white cells and platelets. 26 , 27 By using nonleukoreduced blood, unique data were provided on storage and radiation effects on platelets, WBC, and differential WBC count. While platelet function was evaluated, functional assessments of white cells were not performed.…”

Section: Discussionmentioning

confidence: 99%

Cellular and Biochemical Effects of Combined X-Ray Radiation and Storage on Whole Blood

et al. 2022

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Background Evaluating the impact of ionizing radiation on stored blood is relevant since blood banks are major assets in emergency conditions such as radiation incident/attack. This study aimed to fill our knowledge gap of combined radiation and storage effects on blood. Methods Blood collected from 16 anesthetized rats was anticoagulated, aliquoted into storage bags, and assigned to 8 groups using protocols combining storage (1-day vs 3-day 4oC) plus irradiation (75 Gy vs 0 Gy - control). Bags were positioned inside an X-ray irradiator (MultiRad-350). Complete blood count, differential white blood cell count, biochemistry, and hemostasis were analyzed (≥7 bags/group). Results Na+, bicarbonate, glucose, and pH significantly reduced, while K+, Cl−, and lactate increased by storage. Coagulation measures were not significantly altered after radiation. White blood cell count and most cell types were numerically reduced after radiation, but changes were statistically significant only for monocytes. No significant alterations were noted in aggregation or rotational thromboelastometry parameters between irradiated and control. Conclusions Evaluating cellular/biochemical parameters aids in assessing stored blood adequacy after radiation. Data suggest that fresh or cold-stored blood can sustain up to 75 Gy without major critical parameter changes and may remain suitable for use in critically ill patients in military/civilian settings.

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Section: Discussionmentioning

confidence: 99%

Cellular and Biochemical Effects of Combined X-Ray Radiation and Storage on Whole Blood

et al. 2022

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“…Blood withdrawal was performed by jugular venipuncture with a previously described protocol [ 22 ]. Briefly, blood volume was withdrawn by gravity using an analytical scale.…”

Section: Methodsmentioning

confidence: 99%

Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep

Sousa

Oliveira

et al. 2022

Veterinary Sciences

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Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants.

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“…The blood storage bags used in veterinary medicine were developed for the storage of human blood, however studies have been evaluating the viability of such blood bags for the storage of blood of different animal species [ 7 , 8 ]. The CPDA-1 bags contain a solution of citrate, phosphate, dextrose, and adenine (63 mL) that allows the storage of human blood for 35 days, while the CPD/SAG-M bags, in addition to citrate, phosphate, and dextrose (63 mL) have an additive solution in a satellite bag containing saline, adenine, and mannitol (100 mL) that provides a greater supply of energy and phosphate for the survival of red blood cells.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Barros

Sousa

Tavares

et al. 2021

Biology

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Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

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The Effects of Pre-Storage Leukoreduction on the Conservation of Bovine Whole Blood in Plastic Bags

Cited by 3 publications

References 32 publications

Cellular and Biochemical Effects of Combined X-Ray Radiation and Storage on Whole Blood

Cellular and Biochemical Effects of Combined X-Ray Radiation and Storage on Whole Blood

Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep

Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

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