The effects of pregnancy and parturition on the substance P content of the rat uterus: uterine growth is accompanied by hypertrophy of its afferent innervation

Amira, Smain; Morrison, J.; Rayfield, K. M.

doi:10.1113/expphysiol.1995.sp003874

Cited by 15 publications

(4 citation statements)

References 18 publications

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“…However, there are species differences, since no comparable degeneration of the afferent innervation occurs in the rat uterus during pregnancy (Traurig et al, 1984). Indeed, Amira et al (1995) have reported hypertrophy of the afferent innervation in the rat uterus during pregnancy. The present molecular data show that PPT-B, the gene that encodes NKB, is expressed in the human uterus and indicate that NKB is the predominant tachykinin associated with the human uterus.…”

Section: E Patak Et Almentioning

confidence: 99%

Tachykinins and tachykinin receptors in human uterus

Patak

Candenas

Pennefather

et al. 2003

British J Pharmacology

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1 Studies were undertaken to determine the nature of the receptors mediating contractile effects of tachykinins in the uteri of nonpregnant women, and to analyse the expression of preprotachykinins (PPT), tachykinin receptors and the cell-surface peptidase, neprilysin (NEP), in the myometrium from pregnant and nonpregnant women. 2 The neurokinin B (NKB) precursor PPT-B was expressed in higher levels in the myometrium from nonpregnant than from pregnant women. Faint expression of PPT-A mRNA was detectable in the myometrium from nonpregnant but not pregnant women. PPT-C, the gene encoding the novel tachykinin peptide hemokinin-1 (HK-1), was present in trace amounts in the uteri from both pregnant and nonpregnant women. 3 Tachykinin NK 2 receptors were more strongly expressed in tissues from nonpregnant than from pregnant women. NK 1 receptor mRNA was present in low levels in tissues from both pregnant and nonpregnant women. A low abundance transcript corresponding to the NK 3 receptor was present only in tissues from nonpregnant women. 4 The mRNA expression of the tachykinin-degrading enzyme NEP was lower in tissues from nonpregnant than from pregnant women. 5 Substance P (SP), neurokinin A (NKA) and NKB, in the presence of the peptidase inhibitors thiorphan, captopril and bestatin, produced contractions of myometrium from nonpregnant women. The order of potency was NKAbSPXNKB. The potency of NKA was unchanged in the absence of peptidase inhibitors. Nle 10 ]NKA(4 -10). 8 These data are consistent with a role of tachykinins in the regulation of human uterine function, and reinforce the importance of NK 2 receptors in the regulation of myometrial contraction.

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Section: E Patak Et Almentioning

confidence: 99%

Tachykinins and tachykinin receptors in human uterus

Patak

Candenas

Pennefather

et al. 2003

British J Pharmacology

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“…In the rat, Traurig et al 31 observed no change in the SP-IR content of the uterus during the oestrous cycle, after ovariectomy or during pregnancy, while another study reported an increase in the SP-IR concentration during gestation. 38 A study by Robbins et al, 39 although not designed to study transmitter content directly, indicated that response sensitivity of the afferent nerves was affected by the hormonal variations occurring throughout the oestrous cycle.…”

Section: Occurrence and Distribution Of Tachykinins In The Uterusmentioning

confidence: 99%

Effects Of Tachykinins On Uterine Smooth Muscle

Patak

Story

2000

Clin Exp Pharma Physio

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1. Sensory nerves supplying the mammalian uterus have been shown to contain substance P (SP) and neurokinin (NK)A. This review presents some of the advances that have led to a greater understanding of the effects of tachykinins on uterine smooth muscle. 2. The cell-surface peptidase neprilysin (EC.3 24.11, endopeptidase 24.11, enkephalinase, CALLA, CD10) has been shown to play a major role in regulating the actions of tachykinins on both rat and human myometrium. Because this peptidase is known to be regulated by steroids and pregnancy, its effects may be of physiological relevance. 3. Tachykinins produce contractions of isolated myometrial preparations from non-pregnant rats and mice. The NK2 receptor mediates these effects in rat uterus, while the NK1 receptor may mediate these effects in the mouse uterus. 4. The effects of tachykinins have been examined on myometrial preparations obtained at Caesarean section from near-term pregnant women. In the presence of the peptidase inhibitors (thiorphan, captopril and bestatin), the mammalian tachykinins SP, NKA and NKB produced concentration-dependent uterine contractions. 5. The order of agonist potency NKA > SP = NKB suggested that NK2 receptors mediate uterine contractions in the human. This was confirmed using the stable analogues [Sar9,Met(O2)11]SP, [Lys5MeLeu9Nle10]NKA(4-10) and [N-MePhe7]NKB, which are NK1, NK2 and NK3 receptor selective, respectively. Only [Lys5MeLeu9Nle10]NKA(4-10) produced concentration-related contractions of human uterine smooth muscle. 6. The experimental findings described in the present review, taken together with results published previously in the literature, indicate that tachykinin peptides may play a physiological or pathophysiological role in regulating uterine smooth muscle activity. However, more extensive research will be required to confirm such a role for these peptides.

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“…Uterine sympathetic nerves undergo a profound remodelling in response to physiological and experimental changes in the circulating levels of sex hormones (Owman and Stjernquist 1988;Brauer et al 1992Brauer et al , 1995Zoubina et al 1998Zoubina et al , 2001; however, the influence of sex hormones on uterine parasympathetic and sensory nerves is less well established (Stjernquist et al 1985; Alm and Lundberg 1988;Owman and Stjernquist 1988;Amira et al 1995;Haase et al 1997;Zoubina et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Plasticity in developing rat uterine sensory nerves: the role of NGF and TrkA

Chalar

Richeri

Viettro

et al. 2003

Cell and Tissue Research

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In the present study we investigated the effects of infantile/prepubertal chronic oestrogen treatment, chemical sympathectomy with guanethidine and combined sympathectomy and chronic oestrogen treatment on developing sensory nerves of the rat uterus. Changes in sensory innervation were assessed quantitatively on uterine cryostat tissue sections stained for calcitonin gene-related peptide (CGRP). Uterine levels of NGF protein, using immunohistochemistry and ELISA, and mRNA, using Northern blots and in situ hybridization, were also measured. Finally, levels of TrkA NGF receptor in sensory neurons of T13 and L1 dorsal root ganglia (DRG), which supply the uterus, were assessed using densitometric immunohistochemistry. These studies showed that: (1) chronic oestrogen treatment led to an 83% reduction in the intercept density of CGRP-immunoreactive nerves; (2) sympathectomy had no effect on the density of uterine sensory nerves or on the pattern of oestrogen-induced changes; (3) NGF mRNA and protein increased following sympathectomy or chronic oestrogen treatment; and (4) oestrogen produced increased intensity of labelling (28%) for TrkA receptors in small-diameter sensory neurons, but decreased labelling (13%) in medium-sized neurons, which represent the large majority of the DRG neurons supplying the upper part of the uterine horn. Contrary to expectations, increased levels of NGF after sympathectomy and oestrogen treatment did not lead to increased sensory innervation of the uterus. The possibility that alterations in neuronal levels of TrkA contribute to the lack of response of uterine sensory nerves to the oestrogen-induced increase in NGF levels is discussed.

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The effects of pregnancy and parturition on the substance P content of the rat uterus: uterine growth is accompanied by hypertrophy of its afferent innervation

Cited by 15 publications

References 18 publications

Tachykinins and tachykinin receptors in human uterus

Tachykinins and tachykinin receptors in human uterus

Effects Of Tachykinins On Uterine Smooth Muscle

Plasticity in developing rat uterine sensory nerves: the role of NGF and TrkA

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