The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress

Abstract

Background:Stress in infancy has dramatic effects on different systems, including the nervous system, endocrine, immune, reproductive and etc.Objective:The purpose of this study was to investigate the effects of extract of Iranian propolis (EIP) on ovarian tissue and oxidative stress in rats with maternal separation stress.Materials and Methods:48 immature female rats were divided randomly into six groups. 1) Control group, 2) Control group+saline, 3) Stress group, includes infants that were separated from their mothers 6 hr/day, the 4th, 5th and 6th groups consisted of infants who in addition to daily stress received 50, 100 and 200 mg/kg of EIP, respectively. Then serum corticosterone, 17-beta-estradiol, malondialdehyde, total superoxide dismutase, glutathione peroxidase and ferric reducing antioxidant power levels were measured. The ovarian sections were stained by H&E, PAS, and TUNEL methods and were studied with optical microscopy. Results:Stress increased the blood serum corticosterone levels and 17-beta-estradiol reduced significantly (p<0.001) and EIP prevented from this changes (p<0.01). EIP significantly increased the number of ovarian follicles, oocytes and oocytes diameter in neonatal rat following stress (p<0.01). EIP also significantly decreased the number of atretic follicles, TUNEL+granulosa cells, malondialdehyde levels and increased ferric reducing antioxidant power, total superoxide dismutase and glutathione peroxidase serum levels in neonatal rats following stress. The dose of 200 mg/kg EIP was more effective.Conclusion:This Study showed that the Iranian Propolis significantly could prevent oxidative stress and histopathological changes in the ovary of the neonatal rat the following stress.

Progesterone exerts antidepressant-like effect in a mouse model of maternal separation stress through mitigation of neuroinflammatory response and oxidative stress

“…(Thirunavukkarasu et al 2012;Nouri et al 2017). In our study, the levels of MDA were significantly increased and the content of antioxidant capacity markedly decreased in MS mice, which indicates lipid proxidation and oxidative stress in the hippocampus which are in agreement with prior studies (Arabameri et al 2017;Menezes et al 2017). Treatment with progesterone not only increased antioxidant capacity, but also decreased the MDA level in the hippocampus of MS mice.…”

Propolis as a Potential Disease-Modifying Strategy in Parkinson’s disease: Cardioprotective and Neuroprotective Effects in the 6-OHDA Rat Model

“…Sham animals received saline injection at the same brain points. Twenty-four hours after stereotaxic surgery, animals in the groups with the +P termination received daily gavages with Brazilian green propolis (200 mg/kg) [ 53 , 54 , 55 ], while rats in the other groups received filtered water for 40 days. Rats were weighed once a week.…”

“…On the first day after the 6-OHDA injection, propolis solution was administered at a dose of 200 mg/kg [ 53 , 54 , 55 ] to the sham + P and 6-OHDA + P animals and filtered water to the others until the 40th day. The standardized propolis extract (EPP-AF ® , Ribeirão Preto, São Paulo, Brazil) employed in this study was kindly provided by Apis Flora Co. and is a composition containing mostly green propolis in water-soluble dry extract form [ 58 ].…”

Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application

“…Arabameri et al [ 64 ] proved that the Iranian propolis could significantly prevent oxidative stress (by alleviating the changes in ferric-reducing antioxidant power, SOD, GPx, and MDA) as well as histopathological changes (the number of ovarian follicles, oocytes, and oocytes diameter) in the ovaries of the neonatal rat following maternal separation stress (infants were separated from their mothers 6 hours per day). All three applied doses exerted positive effect, but the most effective was the dose of 200 mg/kg ( Table 2 ).…”