The effects of Securidaca longepedunculata root extract on ionic currents and contraction of cultured rat skeletal muscle cells

Effect ofSecuridaca longepedunculata. (Polygalaceae) Root Extract on Intracellular Calcium Transient in Cultured Rat Skeletal Muscle Cells

“…SL applied alone increased the intracellular free calcium level. The partial reversibility agrees with the results of Kone (1980) and Mouzou (1999). Data and reasoning can be summarized as follows: a) IP 3 receptors were not involved in the SL effect, as the induced calcium increase remained even in the presence of 2-aminoethoxydiphenylborate (2-APB), an inhibitor of calcium-releasing IP 3 receptors (Diver et al, 2001;Powell et al, 2001).…”

“…The root extract of SL increases the sodium current and contraction of cultured rat skeletal muscle cells (Mouzou et al, 1999). In previous work, we have shown an important augmentation of cultured rat skeletal muscle cell contraction without a significant effect on L-type calcium current (Mouzou et al, 1999). This initial finding suggests an exploration of the possible interference of the lyophilized root extract with calcium mobilizing systems in rat skeletal muscle cells.…”

“…The root extract has been shown to have many effects on frog muscle fiber, such as a curare-like effect at the neuromuscular junction, an increase in action potential duration, and some competition with the Naja nigricollis Reinhardt (Elapidae) venom (Kone, 1980). The root extract of SL increases the sodium current and contraction of cultured rat skeletal muscle cells (Mouzou et al, 1999). In previous work, we have shown an important augmentation of cultured rat skeletal muscle cell contraction without a significant effect on L-type calcium current (Mouzou et al, 1999).…”

“…The lyophilized extract was added directly to the control solution at 1 mg=ml to make the test solution (Mouzou et al, 1999). Rapid changes of solution in the surroundings of tested cells were achieved by means of a homemade (original) microperfusion system.…”

Anti-inflammatory activity of extracts of root bark of <i>Securidaca longipedunculata</i> fres (polygalaceae)

“…The cut root possesses a strong aromatic odour which strongly suggests the presence of volatile constituents. Pharmacologically, root extracts of the plant increased sodium ion currents and enhanced the contractile response elicited by durable depolarization thus suggesting the possibility of one or more of the constituents acting on the voltage-sensor of excitationcontraction coupling in rat skeletal muscles (Mouzou et al, 1999). Extract of the root bark of S. longipedunculata exhibited neuromuscular blocking and negative inotropic and chronotropic cardiac effects (Ojewole et al, 2000) and also demonstrated spasmolytic activity on vascular and extravascular smooth muscles in experimental animals (Ojewole et al, 2001).…”