The effects of silver nanoparticles on RAW 264 7  Macrophages and human whole blood cell cultures

Lategan, Kim Leigh; Walters, Chavon; Pool, Edmund John

doi:10.2741/4722

Cited by 14 publications

(2 citation statements)

References 27 publications

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“…In murine macrophage cell lines (RAW) biomarkers associated with inflammation were stimulated using ca. 25 µg/mL of Ag NPs coated with PVP [79]. In addition, our results showed that the toxic behavior was evident at low doses of conventional Ag NPs.…”

Section: 𝑤 =supporting

confidence: 49%

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

et al. 2022

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The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability.

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Section: 𝑤 =supporting

confidence: 49%

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

et al. 2022

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“…These downstream processes are dependent on the intrinsic properties of NPs and the activation states of macrophages [249]. Commercial silver NPs have been reported to induce inflammation biomarker expression in murine RAW 264.7 macrophages and anti-inflammation biomarker expression in human whole blood cell cultures [251]. Gold NPloaded mesoporous silica has been demonstrated to induce M2 polarization and promoted the secretion of anti-inflammatory factors of macrophages [252].…”

Section: Cellular Interactions Of Metal/metal Oxide Nanoparticlementioning

confidence: 99%

Biocompatibility of nanomaterials and their immunological properties

et al. 2021

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Nanomaterials (NMs) have revolutionized multiple aspects of medicine by enabling novel sensing, diagnostic, and therapeutic approaches. Advancements in processing and fabrication have also allowed significant expansion in the applications of the major classes of NMs based on polymer, metal/metal oxide, carbon, liposome, or multi-scale macro-nano bulk materials. Concomitantly, concerns regarding the nanotoxicity and overall biocompatibility of NMs have been raised. These involve putative negative effects on both patients and those subjected to occupational exposure during manufacturing. In this review, we describe the current state of testing of NMs including those that are in clinical use, in clinical trials, or under development. We also discuss the cellular and molecular interactions that dictate their toxicity and biocompatibility. Specifically, we focus on the reciprocal interactions between NMs and host proteins, lipids, and sugars and how these induce responses in immune and other cell types leading to topical and/or systemic effects.

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Engineered Nanomaterials

Lin

2023

Patty's Toxicology

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Engineered nanomaterials (ENMs) are intentionally designed materials of ≤100 nm such as titanium dioxide nanoparticles, cerium oxide nanoparticles, iron oxide nanoparticles, silver or gold nanoparticles, carbon family nanomaterials (graphene, quantum dots, nanohorns, nanotubes, fullerene/buckyballs), transitional metal dichalcogenide nanoparticles, or silica nanoparticles. These have entered our lives through industrial and consumer products, or workplace exposure. As learned industrial professionals, we can deal with this influx through a better understanding of ENMs themselves, and their underlying potential toxicity. In spite of ever‐changing rules due to an improved understanding of the toxicological and environmental impact of ENMs, it is also extremely important to understand current EU and U.S. regulations with regard to workplace exposure limits as well as how to better measure and manage the health risks. In this updated chapter, we cover the various types of ENMs, potential health effects such as cardiovascular, pulmonary, skin, gut, and immune toxicity, exposure assessment, and occupational risk management.

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The effects of silver nanoparticles on RAW 264 7 Macrophages and human whole blood cell cultures

Abstract: Introduction 3. Material and methods 3.1. Characterization of silver nanoparticles 3.2. Preparation of silver nanoparticles 3.

Cited by 14 publications

References 27 publications

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

Biocompatibility of nanomaterials and their immunological properties

Engineered Nanomaterials

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