The effects of sinomenine on intestinal absorption of paeoniflorin by the everted rat gut sac model

Chan, Kelvin; Liu, Zhong Qiu; Jiang, Zhihong; Zhou, Hua; Wong, Yuen Fan; Xu, Hong‐Xi; Liu, Liang

doi:10.1016/j.jep.2005.08.020

Cited by 64 publications

(60 citation statements)

References 23 publications

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“…The former technique allows for the direct detection of COU potentially produced in situ. The latter is a useful screening tool for studying the digestive absorption and metabolic behavior of substances introduced in the various parts of the gastrointestinal tract (10). In addition, this last technique allows the CA4H activity of recombinant yeasts to be tested using the lowest nontoxic dose of CIN (7 mol).…”

Section: Discussionmentioning

confidence: 99%

RecombinantSaccharomyces cerevisiaeStrain Expressing a Model Cytochrome P450 in the Rat Digestive Environment: Viability and Bioconversion Activity

Garrait

Jarrige

Blanquet

et al. 2007

Appl Environ Microbiol

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An innovative "biodrug" concept, based on the oral administration of living recombinant microorganisms, has recently emerged for the prevention or treatment of various diseases. An engineered Saccharomyces cerevisiae strain expressing plant P450 73A1 (cinnamate-4-hydroxylase [CA4H] activity) was used, and its survival and ability to convert trans-cinnamic acid (CIN) into p-coumaric acid (COU) were investigated in vivo. In rats, the recombinant yeast was resistant to gastric and small intestinal secretions but was more sensitive to the conditions found in the large intestine. After oral administration of yeast and CIN, the CA4H activity was shown in vivo, with COU being found throughout the rat's digestive tract and in its urine. The bioconversion reaction occurred very fast, with most of the COU being produced within the first 5 min. The gastrointestinal sac technique demonstrated that the recombinant yeast was able to convert CIN into COU (conversion rate ranging from 2 to 5%) in all the organs of the rat's digestive tract: stomach, duodenum, jejunum, ileum, cecum, and colon. These results promise new opportunities for the development of drug delivery systems based on engineered yeasts catalyzing a bioconversion reaction directly in the digestive tract.

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Section: Discussionmentioning

confidence: 99%

RecombinantSaccharomyces cerevisiaeStrain Expressing a Model Cytochrome P450 in the Rat Digestive Environment: Viability and Bioconversion Activity

Garrait

Jarrige

Blanquet

et al. 2007

Appl Environ Microbiol

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show abstract

“…The mechanism underlying this improvement of bioavailability of paeoniflorin may be explained by, that sinomenine could decrease the efflux transport of paeoniflorin by P-glycoprotein in the small intestine [57] .…”

Section: Sinomeninementioning

confidence: 99%

Bioavailability enhancers of herbal origin: An overview

Kesarwani

Gupta

2013

Asian Pacific Journal of Tropical Biomedicine

398

197

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“…The SM·HCl (90 mg/ kg) significantly improves the bioavailability of paeoniflorin in rats (Liu et al, 2005a), while coadministration of paeoniflorin does not affect the pharmacokinetic parameters and tissue distribution of sinomenine (Liu et al, 2005b). The mechanism is suggested that sinomenine decreases the efflux transport of paeoniflorin by P-glycoprotein (Chan et al, 2006).…”

Section: Pharmacokineticsmentioning

confidence: 99%

Sinomenium acutum: A review of chemistry, pharmacology, pharmacokinetics, and clinical use

Zhao¹,

Peng²,

Zhang³

et al. 2012

Pharmaceutical Biology

125

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The effects of sinomenine on intestinal absorption of paeoniflorin by the everted rat gut sac model

Cited by 64 publications

References 23 publications

RecombinantSaccharomyces cerevisiaeStrain Expressing a Model Cytochrome P450 in the Rat Digestive Environment: Viability and Bioconversion Activity

RecombinantSaccharomyces cerevisiaeStrain Expressing a Model Cytochrome P450 in the Rat Digestive Environment: Viability and Bioconversion Activity

Bioavailability enhancers of herbal origin: An overview

Sinomenium acutum: A review of chemistry, pharmacology, pharmacokinetics, and clinical use

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