The effects of strength training and raloxifene on bone health in aging ovariectomized rats

Stringhetta-Garcia, Camila Tami; Singulani, Monique Patrício; Santos, Leandro Figueiredo dos; Louzada, Mário Jefferson Quirino; Nakamune, Ana Cláudia Stevanato; Chaves‐Neto, Antônio Hernandes; Rossi, Ana Cláudia; Ervolino, Edílson; Dornelles, Rita Cássia Menegati

doi:10.1016/j.bone.2015.11.023

Cited by 30 publications

(33 citation statements)

References 52 publications

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“…An analysis of two histological sections from each animal was performed at ×400 magnification. The immunolabeling pattern was evaluated using scores, adapted from the criteria established by Stringhetta‐Garcia et al, (). For BMP 2/4, OCN, and VEGF, SCORE 0 means total absence of immunolabeling; SCORE 1 means low labeling, considering that approximately 1/3 of the cells were immunoreactive and that there was a light labeling in the extracellular matrix; SCORE 2 means moderate labeling, considering that approximately 1/2 of the cells were immunoreactive and that there was a moderate labeling in the extracellular matrix; SCORE 3 means high labeling, considering that approximately 2/3 of the cells were immunoreactive and that there was a strong labeling in the extracellular matrix.…”

Section: Methodsmentioning

confidence: 99%

Effects of leukocyte‐ and platelet‐rich fibrin associated or not with bovine bone graft on the healing of bone defects in rats with osteoporosis induced by ovariectomy

Engler-Pinto

Siéssere

Calefi

et al. 2019

Clinical Oral Implants Res

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Objective To evaluate the healing of critical size defects (CSDs) in calvaria of rats with osteoporosis induced by ovariectomy and treated with leukocyte‐ and platelet‐rich fibrin (L‐PRF) associated or not with bovine bone graft (XENO). Material and Methods A total of 32 rats underwent a bilateral ovariectomy procedure. After 3 months, one 5 mm in diameter CSD was created in the middle of the calvaria of each animal. In group C, defect was filled with blood clot only. In PRF, XENO, and PRF‐XENO groups, defects were filled with 0.1 ml of L‐PRF, 0.1 ml of XENO, and a mixture of 0.1 ml of L‐PRF plus 0.1 ml of XENO, respectively. L‐PRF compressed clots were used to cover the defects in PRF and PRF‐XENO groups. Animals were submitted to euthanasia at 30 postoperative days. Histomorphometric, microtomographic, and immunohistochemical analyses were performed. Results PRF‐XENO group presented greater amount of neoformed bone (NB) when compared with XENO group, as well as higher expression of vascular endothelial growth factor (VEGF), osteocalcin (OCN), and bone morphogenetic protein (BMP‐2/4) (p < .05). PRF group presented increased amount of NB and higher expression of VEGF, OCN, BMP‐2/4, and Runt‐related transcription factor 2 (RUNX‐2) when compared with group C (p < .05). Conclusions (a) The isolated use of L‐PRF clot can improve bone neoformation in CSDs in rats with osteoporosis induced by ovariectomy, but seems to lead to decreased amount of bone neoformation when compared to the isolated use of XENO; (b) L‐PRF potentiates the healing of XENO in rats with osteoporosis induced by ovariectomy.

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Section: Methodsmentioning

confidence: 99%

Effects of leukocyte‐ and platelet‐rich fibrin associated or not with bovine bone graft on the healing of bone defects in rats with osteoporosis induced by ovariectomy

Engler-Pinto

Siéssere

Calefi

et al. 2019

Clinical Oral Implants Res

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“…The dilution of primary antibodies was based on a titration test. Immunohistochemical processing followed the protocol described by Stringhetta-Garcia et al (2016).…”

Section: Histomorphometry Osteocyte Number and Immunohistochemistrymentioning

confidence: 99%

llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause

Pereira

Stringhetta-Garcia

Xavier

et al. 2017

Experimental Gerontology

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During perimenopause, oxidative stress increases, which may result in disruption of bone turnover, and consequently in osteoporosis. The use of antioxidants may be an effective nutritional approach to reducing osteoporosis in this period of life. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. Our hypothesis was that MT would decrease oxidative stress and mitigate bone deterioration. To test this, we analyzed oxidative stress markers of bone turnover, and local and systemic markers of bone metabolism of rats during natural perimenopause. Female Wistar rats (aged 16months) in proven perimenopause period received 20mg/kgBW/day of mate tea, by gavage (PM+MT Group, n=10) or water (PM Group, n=10). Female rats aged 4months (AD Group, n=10) received water. The treatment period was four weeks. MT minimized the deterioration of rat microarchitecture, characterized by increase in the bone trabecular area, number of osteocytes and areal bone mineral density. These results were accompanied by a lower level of malondialdehyde, an oxidative stress marker, in femoral tissue homogenate. Plasmatic tartrate-resistant acid phosphatase, a typical osteoclastic function marker, decreases after treatment, indicating a decrease in osteoclastic function. MT also modified the immunostaining pattern of bone metabolism markers, decreasing the receptor activator of nuclear factor kappa-B ligant (RANKL), superoxide dismutase isoform 2 (SOD2) and increasing osteoprotegerin (OPG), a decoy receptor for the RANKL, which positively modulates bone mass. These results suggested MT was capable of decreasing bone resorption by inhibiting the osteoclastogenesis in a RANKL-dependent signaling pathway activated by oxidative stress. Taken together, the results indicated that MT minimized bone loss in perimenopause and this effect is at least partly due to the decrease in oxidative stress, confirming our hypothesis.

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“…The animals were familiarized with a climbing ladder (1.1 × 0.18 m, 2-cm grid, 80°incline-Engineered by Department of maintenance -São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazill) for 3 days, based on previous studies [23,26]. The adaptation period was composed by 3 sessions, in non-consecutive days, without load.…”

Section: Strength Training Protocol and Maximum Voluntary Carrying Camentioning

confidence: 99%

Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

et al. 2016

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We evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3× week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm/point, 3 points, spot light 0.028 cm, 14 J/cm) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student's t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-α levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-α to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration.

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The effects of strength training and raloxifene on bone health in aging ovariectomized rats

Cited by 30 publications

References 52 publications

Effects of leukocyte‐ and platelet‐rich fibrin associated or not with bovine bone graft on the healing of bone defects in rats with osteoporosis induced by ovariectomy

Effects of leukocyte‐ and platelet‐rich fibrin associated or not with bovine bone graft on the healing of bone defects in rats with osteoporosis induced by ovariectomy

llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause

Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

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