The effects of streptozotocin-induced diabetes on brucellosis of rats

Abstract

It is believed that an infection is more common and runs a more protracted course in people with diabetes. In clinical practice, it is important to be aware of these associations, as the prognosis is often dependent upon prompt recognition and appropriate treatment. To show the course of brucellosis in the diabetic state, a model of Brucella melitensis infection was used in the setting of streptozotocin-induced diabetes in rat. B. melitensis infection proceeded more severely in diabetic rats and the severity of diabetes affected the prognosis. However, no association was found between B. melitensis and insulin using in vitro and in vivo experiments. Our study illustrates that B. melitensis infection in diabetes should be taken seriously.

Laboratory Animal Models for Brucellosis Research

“…Despite the fact that rats do not develop physical signs of infection and are considered more resistant to infection than mice, they develop persistent bacteremia and do not have spontaneous cure after one month of infection [69, 70]. Therefore, rats have been selected as an experimental model for evaluation of increased susceptibility to infection (including Brucella infection) in patients with chronic disorders.…”

“…In this case, diabetes is induced by streptozotocin before challenge with B. melitensis. Diabetic rats have higher numbers of bacteria in the liver and spleen when compared to control rats [69]. Other studies investigated the effect of chronic ethanol consumption on the course of B. melitensis infection in a rat model [71–73].…”

Preclinical Models of Brucellar Spondylodiscitis

“…Mice, rats, rabbits, and sheep represent the available candidates to induce Brucellosis [23][24][25][26]. However, the preclinical model of Brucellar spondylodiscitis with a possible high translational efficiency has only been established in rabbits so far [14].…”

“…The rats, more resistant to Brucella infection than the mice [15], can develop the persistent bacteremia, and do not have a spontaneous cure over the 1-month infection [23]. Therefore, rats can serve as a model candidate to evaluate the increased susceptibility to Brucella infection, which mimics the chronic symptoms in patients.…”

Seguimiento clínico, serológico y mediante la reacción de polimerasa en cadena de una familia con brucelosis

“…El defecto en la producción de IL-12 afectó en consecuencia la producción de IFN-γ y la actividad bactericida de los monocitos contra estas bacterias intracelulares 24 . En el caso de Brucella, se ha reportado que ratas Wistar a las que se les indujo la diabetes mediante estreptozotocina, presentaron una infección más grave por B. melitensis que el grupo de ratas no diabéticas 25 . Más recientemente, se reportó que B. abortus tiene preferencia de sobrevivir y replicarse dentro de un cierto tipo de macrófagos (llamados macrófagos activados alternativamente, AAMs) durante el estado crónico de la infección en ratón.…”