The effects of surface properties of liposomes on their activity against Pseudomonas aeruginosa PAO-1 biofilm

Ibaraki, Hisako; Kanazawa, Takanori; Chien, Wan-Yi; Nakaminami, Hidemasa; Aoki, Masakazu; Ozawa, Kosuke; Kaneko, Hiroshi; Takashima, Yuuki; Noguchi, Hiromitsu; Seta, Yasuo

doi:10.1016/j.jddst.2020.101754

Cited by 27 publications

(25 citation statements)

References 51 publications

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“…Taking this into account, the selection of lipid compositions able to improve the interaction and accumulation within the bacterial biofilm together with an enhanced release of loaded antibiotic in situ must be considered and optimized. Moreover, the particle size, bilayer morphology, surface properties, and encapsulation efficacy are also important characteristics to take into consideration [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms

et al. 2021

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Staphylococcus aureus biofilm-associated infections are a major public health concern. Current therapies are hampered by reduced penetration of antibiotics through biofilm and low accumulation levels at infected sites, requiring prolonged usage. To overcome these, repurposing antibiotics in combination with nanotechnological platforms is one of the most appealing fast-track and cost-effective approaches. In the present work, we assessed the potential therapeutic benefit of three antibiotics, vancomycin, levofloxacin and rifabutin (RFB), through their incorporation in liposomes. Free RFB displayed the utmost antibacterial effect with MIC and MBIC50 below 0.006 µg/mL towards a methicillin susceptible S. aureus (MSSA). RFB was selected for further in vitro studies and the influence of different lipid compositions on bacterial biofilm interactions was evaluated. Although positively charged RFB liposomes displayed the highest interaction with MSSA biofilms, RFB incorporated in negatively charged liposomes displayed lower MBIC50 values in comparison to the antibiotic in the free form. Preliminary safety assessment on all RFB formulations towards osteoblast and fibroblast cell lines demonstrated that a reduction on cell viability was only observed for the positively charged liposomes. Overall, negatively charged RFB liposomes are a promising approach against biofilm S. aureus infections and further in vivo studies should be performed.

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Section: Resultsmentioning

confidence: 99%

Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms

et al. 2021

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“…We previously reported that intravenous administration of the block-polymer micelle MPEG-PCL-CH2R4H2C and the cytoplasm-sensitive peptide STR-CH2R4H2C had strong therapeutic efficacy against inflammatory diseases such as RA and atopic dermatitis, and cancer [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. These molecules were designed in our laboratory as novel drug carriers.…”

Section: Introductionmentioning

confidence: 99%

“…These molecules were designed in our laboratory as novel drug carriers. We also indicated that liposomal surface properties are strongly affected to improve the delivery efficacy for the treatment of atopic dermatitis and the activity against biofilm [ 34 , 35 , 36 ]. However, few studies have comprehensively investigated the physical properties of intravenously administered nanocarriers that are highly effective at treating inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Fluorescence Imaging of Passive Inflammation Site Accumulation of Liposomes via Intravenous Administration Focused on Their Surface Charge and PEG Modification

et al. 2021

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Nanocarriers such as liposomes have been attracting attention as novel therapeutic methods for inflammatory autoimmune diseases such as rheumatoid arthritis and ulcerative colitis. The physicochemical properties of intravenously administered nanomedicines enable them to target inflamed tissues passively. However, few studies have attempted to determine the influences of nanoparticle surface characteristics on inflammation site accumulation. Here, we aimed to study the effects of polyethylene glycol (PEG) modification and surface charge on liposome ability to accumulate in inflammatory sites and be uptake by macrophages. Four different liposome samples with different PEG modification and surface charge were prepared. Liposome accumulation in the inflammation sites of arthritis and ulcerative colitis model mice was evaluated by using in vivo imaging. There was greater PEG-modified than unmodified liposome accumulation at all inflammation sites. There was greater anionic than cationic liposome accumulation at all inflammation sites. The order in which inflammation site accumulation was confirmed was PEG-anionic > PEG-cationic > anionic > cationic. PEG-anionic liposomes had ~2.5× higher fluorescence intensity than PEG-cationic liposomes, and the PEG-liposomes had ~2× higher fluorescence intensity than non-PEG liposomes. All liposomes have not accumulated at the inflammation sites in healthy mice. Furthermore, cationic liposomes were taken up to ~10× greater extent by RAW264.7 murine macrophages. Thus, PEG-cationic liposomes that have the ability to accumulate in inflammatory sites via intravenous administration and to be taken up by macrophages could be useful.

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“…Thus, liposome formulations are ideally suited for gastrointestinal infections via oral delivery. In the stomach, the acidic pH leads to phage protein denaturation, while enzymes in the gut degrade phage particles (63,64,65). In chickens, three Salmonella phages were observed to be more stable in gastric fluid (in vitro) and hence determined to have a longer duration of efficacy; one myxovirus and two podoviridae were protected from degradation when encapsulated inside cationic lipid particles, which additionally extended residence time in the animals (57).…”

Section: Liposomesmentioning

confidence: 99%

“…Liposomes have been shown to penetrate bacterial biofilms to access the site of infection, which is often a problem for conventional antibiotics ( 59 , 60 ). Aside from this, liposome encapsulation helps to retain phages at the infection site compared to nonencapsulated ones.…”

Section: Phage Delivery Systemsmentioning

confidence: 99%

Encapsulation and Delivery of Therapeutic Phages

Loh

Gondil

Manohar

et al. 2021

Appl Environ Microbiol

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Delivery of therapeutic compounds to the site of action is crucial. While many chemical substances such as beta-lactam antibiotics can reach therapeutic levels in most parts throughout the human body after administration, substances of higher molecular weight such as therapeutic proteins may not be able to reach the site of action (e.g. an infection), and are therefore ineffective. In the case of therapeutic phages, i.e. viruses that infect microbes that can be used to treat bacterial infections, this problem is exacerbated; not only are phages unable to penetrate tissues, but phage particles can be cleared by the immune system and phage proteins are rapidly degraded by enzymes or inactivated by the low pH in the stomach. Yet, the use of therapeutic phages is a highly promising strategy, in particular for infections caused by bacteria that exhibit multi-drug resistance. Clinicians increasingly encounter situations where no treatment options remain available for such infections, where antibiotic compounds are ineffective. While the number of drug-resistant pathogens continues to rise due to the overuse and misuse of antibiotics, no new compounds are becoming available as many pharmaceutical companies discontinue their search for chemical antimicrobials. In recent years, phage therapy has undergone massive innovation for the treatment of infections caused by pathogens resistant to conventional antibiotics. While most therapeutic applications of phages are well described in the literature, other aspects of phage therapy are less well documented. In this review, we focus on the issues that are critical for phage therapy to become a reliable standard therapy and describe methods for efficient and targeted delivery of phages, including their encapsulation.

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The effects of surface properties of liposomes on their activity against Pseudomonas aeruginosa PAO-1 biofilm

Cited by 27 publications

References 51 publications

Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms

Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms

In Vivo Fluorescence Imaging of Passive Inflammation Site Accumulation of Liposomes via Intravenous Administration Focused on Their Surface Charge and PEG Modification

Encapsulation and Delivery of Therapeutic Phages

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