The Effects of Systemic Use of Nicotine on the Rat Nasal Mucosa: a Histopathologic and Immunohistochemical Study

Yorgancılar, Ediz; Tunik, Selcuk; Deveci, Engin; Gün, Ramazan; Bakır, Salih; Kınış, Vefa; Ayaz, Ercan; Topçu, İsmail

doi:10.4067/s0717-95022012000300010

Cited by 2 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All morphological changes; congestion in blood vessels, inflammation, damage of collagen fibres, alveolar matrix, VEGF, osteonectin and MMP-2 expression were noted. The intensity of these changes were graded from 0 to 6 (0 -no change, 1 -low, 2intermediate, 3-4 -moderate, 5-6 -intense) [34].…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

et al. 2019

View full text Add to dashboard Cite

Background: The objective of this study was to investigate whether long term formaldehyde inhalation may affect periodontal membrane and alveolar bone loss leading to periodontitis. The negative effects of formaldehyde were described using vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP-2) and osteonectin antibodies involved in the extracellular matrix and angiogenetic development. Materials and methods: Thirty adult Wistar albino rats were used in this study. Rats were divided into two groups: a control group (n = 15) and formaldehyde administered group (n = 15). Formaldehyde group was exposed to inhalation of 10 ppm formaldehyde 8 hours a day, 5 days a week for 5 weeks. Maxillary bone regions were dissected under anaesthesia. After fixation in 10% formaldehyde solution, tissues were passed through graded ethanol series to obtain paraffin blocks. Five-micrometre histological sections were cut with RM2265 rotary microtome stained with Masson trichrome and VEGF, MMP-2 and osteonectin antibodies for examination under Olympus BH-2 light microscopy. Results: The present study revealed that congestion in blood vessels, degeneration of collagen fibres and alveolar matrix around alveolar bone were observed to be more significant in formaldehyde group than the control group (p ≤ 0.001). Interestingly, VEGF expression in the formaldehyde group was the most significant finding between the two groups (p < 0.001). When compared inflammation, MMP-2 and osteonectin expressions were significant (p < 0.01) in the formaldehyde group. Conclusions: It was suggested that formaldehyde toxicity decreased the expression of MMP-2 and in osteoblasts as well as affecting the retention of MMP levels in tooth cavity, which is very low in collagen fibres. But, vice versa for the expression of VEGF in dilated vascular endothelial cells and osteocytes in alveolar bone. As a conclusion, formaldehyde disrupts the periodontal membrane and may cause collagen fibres degeneration by affecting the alveolar bone matrix.

show abstract

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Nicotine is generally administered to the body with the inhalation of tobacco smoke. Also, pharmaceutically available as chewing gum, transdermal patches, or microtablets for stopping smoking as a replacement therapy [9] [10]. The widespread nicotine intake, smoking was shown to have a negative impact on bone healing after fracture as well.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Nikotine Administration Impairs Blood Parameters and Femoral Bone Tissue Structure in an Osteoporosis Model of Rats

Baloğlu¹,

Özkorkmaz²

2019

JBM

View full text Add to dashboard Cite

Osteoporosis is a systemic bone disease that results in the loss of bone mass and impared bone structure. Animal models for osteoporosis are generated by ovariectomy. Adult female Sprague-Dawley rats were divided into control, bilateral ovariectomy and bilateral ovariectomy and subcutaneous nicotine administered (received nicotine sulphate, 2 mg/kg) groups, daily for 28 days. At the end of the period, rats were sacrified under anesthesia blood samples were taken and femoral tissues were dissected. Estrogen, calcium and alkaline phosphatase levels were measured. Tissue samples were prepared for histopathogical examination. Sections were stained with Hematoxylin and Eosin and examined under light microcope. Biochemical parameters were decreased depending on the overiectomy, also decrement was notable with nicotine intake. In the ovariectomy group; increased inflammatory cells with degenerative changes around the femoral compact bone and dilatation of osteon structures in bone trabeculae and apoptotic changes in osteocyte cells in bone lacuna were apparent. In the ovariectomy with nicotine administration group, excessive dilatation of the havers lamellae in the compact bone region, increased osteoclastic activity, picnosis and apoptotic nucleus of the osteoclastic cells located in the lacuna, and increased collagen fibers in the matrix were observed. We suggest that ovariectomy and nicotine administration together effect estrogen and calcium metabolism negatively, stimulate alterations in the structural properties of bone matrix, also affect osteocyte development and bone lamellar structure that may accelerate osteoporosis development.

show abstract

The Effects of Systemic Use of Nicotine on the Rat Nasal Mucosa: a Histopathologic and Immunohistochemical Study

Cited by 2 publications

References 13 publications

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

Long-Term Nikotine Administration Impairs Blood Parameters and Femoral Bone Tissue Structure in an Osteoporosis Model of Rats

Contact Info

Product

Resources

About