The Effects of Testosterone, Oestradiol and Pregnant Mare Serum Gonadotrophin on Growth and Enzyme Activity in the Rat Testis

Elkington, J. S. H.; Blackshaw, A. W.

doi:10.1530/jrf.0.0230001

Cited by 15 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acid and alkaline phosphatases regulate the secretory activity of the testis and are present in seminiferous tubules and Leydig cells (Elkington & Blackshaw, 1970). In the present study decrease in the acid phosphatase activity is seen.…”

Section: Patil S a And Patil S Bsupporting

confidence: 63%

Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice

Patil

2011

Int. J. Morphol.

View full text Add to dashboard Cite

Section: Patil S a And Patil S Bsupporting

confidence: 63%

Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice

Patil

2011

Int. J. Morphol.

View full text Add to dashboard Cite

“…The possible hormonal control of LDH-X activity in the testis was suggested when Elkington and Blackshaw (1970) found that the depression of LDH-X activity in the testis of the intact rat by oestradiol could be prevented by combinations of testosterone and pregnant mare serum gonadotrophin administered with the oestradiol treatment. Recently Winer and Nikitovitch-Winer (1971) have shown that FSH and LH or testosterone alone will maintain LDH-X activity in the testes of rats hypophy->I< Part II, Aust.…”

Section: Introductionmentioning

confidence: 99%

Studies on Testicular Function III. Effects of FSH, LH, Testosterone and Dihydrotestosterone on Restoration and Maintenance of Testicular-Specific Lactate Dehydrogenase Activity in the Hypophysectomized Rat

Elkington¹,

Blackshaw²

1974

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Hypophysectomy of the adult male rat depressed the activity of testicular-specific lactate dehydrogenase (LDH-X). Groups of rats were treated with FSH (F), LH (L) or testosterone (T) alone or in combination after 32 days of post-hypophysectomy regression (restoration experiment) for a further 32 days or with F, T or dihydrotestosterone (D) alone or in combination from 2 days after hypophysectomy (maintenance experiment) for 30 days.LDH-X activity per milligram of protein was restored to normal by the hormonal combinations FT and LT while F, L, or T given alone or the FL and FLT combinations were ineffective. However, the total LDH-X activity per testis was only partially restored by the hormonal combinations FT, LT and FLT.In the maintenance experiment LDH-X activity per milligram of protein was maintained by all hormonal treatments except D, while the total LDH-X activity per testis was completely maintained by the FT hormonal combination. All other hormonal treatments except F alone maintained the activity of this isoenzyme above the level in the hypophysectomized rat.The restoration and maintenance of LDH-X activity in the testis of the hypophysectomized rat was successful with those combinations of hormones that were also able to restore or maintain the numbers of late primary spermatocytes and spermatids. Thus, the level of LDH-X activity in the testis is dependent on the presence of late primary spermatocytes and spermatids and hence this enzyme is a good marker for the production of these cells in the rat testis.

show abstract

“…Lactate dehydrogenase has been used as a marker for active spermatogenesis (Blackshaw andElkington 1970a, 1970b;Elkington and Blackshaw 1970). In this experiment the decrease in LDH-X activity between 8 and 16 days after hypophysectomy began somewhat later than the decrease in the nuclear index for late primary spermatocytes and spermatids, but this may be related to the relative insensitivity of the isoenzyme techniques and does not alter the view that this isoenzyme of LDH is found in the tubular epithelium of the rat testis.…”

Section: ·03 1604·00mentioning

confidence: 89%

“…Studies on the effect of hypophysectomy on enzymes in the testis has concerned mainly the dehydrogenases (see Blackshaw 1970 for references) and lipid metabolism (see Johnson 1970 for references). The testicular hydro lases have received little study in mammals (Elkington and Blackshaw 1971;Vanha-Perttula 1971a, 1971b VanhaPerttula and Nikkanen 1971) but in the testis of the reptile Leptodactylus chaquensis acid phosphatase was located in degenerating cells after hypophysectomy (Bertini et al 1969).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hypophysectomy on Testicular Hydrolases, Lactate Dehydrogenase, and Spermatogenesis in the Rat

Elkington¹,

Blackshaw²,

Jong³

1973

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Hypophysectomy of the adult male rat depressed testis and seminal vesicle weight between 4 and 8 days after hypophysectomy.The activity of soluble acid proteinase (E.C. 3.4.4.23) and ,B-glucuronidase (E.C. 3.2.1.31) increased between 8 and 16 days after the operation, while that for acid phosphatase (E.C. 3.1.3.2) increased until 16 days after hypophysectomy and then decreased to the end of the experiment.There was an increase in the total acid proteinase and ,B-glucuronidase activity per gram testis between 8 and 16 days after hypophysectomy; there was no change in the total activity of acid phosphatase per gram testis until 16-32 days after treatment, when there was a decrease. After hypophysectomy there was a decrease in acid phosphatase activity per testis after 16 days, and acid proteinase activity per testis after 8 days, while there was an increase in both free and total ,B-glucuronidase activity per testis after 16 days. A decrease in lactate dehydrogenase isoenzyme (LDH-X) activity in the testis occurred between 8 and 16 days after the operation.Histochemical evidence showed an increase in acid phosphatase activity in the seminiferous tubules 4-8 days after hypophysectomy, which coincided with a decrease in the yield of round spermatids from late primary sperma tocytes and of long spermatids from round spermatids.It is concluded that lysosomal enzymes of Sertoli cell origin are important in testicular degeneration and, probably, in normal spermatogenesis. Acid phosphatase and acid proteinase are found mainly in spermatocytes and spermatids, while ,B-glucuronidase is found mainly in the Sertoli cells and spermatogonia. LDH-X occurs in the seminiferous tubular epithelium associated with late primary spermatocytes and spermatids.

show abstract

The Effects of Testosterone, Oestradiol and Pregnant Mare Serum Gonadotrophin on Growth and Enzyme Activity in the Rat Testis

Cited by 15 publications

References 14 publications

Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice

Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice

Studies on Testicular Function III. Effects of FSH, LH, Testosterone and Dihydrotestosterone on Restoration and Maintenance of Testicular-Specific Lactate Dehydrogenase Activity in the Hypophysectomized Rat

The Effect of Hypophysectomy on Testicular Hydrolases, Lactate Dehydrogenase, and Spermatogenesis in the Rat

Contact Info

Product

Resources

About