The effects of the electro-photodynamic in vitro treatment on human lung adenocarcinoma cells

Saczko, Jolanta; Nowak, Magdalena; Skołucka, Nina; Kulbacka, Julita; Kotulska, Małgorzata

doi:10.1016/j.bioelechem.2009.12.006

Cited by 24 publications

(16 citation statements)

References 24 publications

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“…What is more, this combined therapy is low invasive and selective. In our previous study the effective action of EP with PDR with hematoporphyrin derivative (HpD) was proven Saczko et al 2010;Kulbacka et al 2011). The electro-photodynamic therapy applied to lung cancer cells (A549) revealed a significant increase of the cytotoxic effect on cells when compared to HpD-PDT alone, even at drug doses significantly lower than typical levels.…”

Section: Discussionmentioning

confidence: 96%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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Two porphyrins, CoTPPS and MnTMPyPCl 5 , were tested for their photodynamic activity and potential novel use in a therapy of human cancers. We investigated an effect of photodynamic reaction (PDR), electroporation (EP) and their combination (electro-photodynamic reaction [EP-PDR]) on human colon adenocarcinoma cell lines (LoVo and resistant to doxorubicin LoVoDX), human breast adenocarcinoma (wild type MCF-7/WT and resistant to doxorubicin MCF-7/DOX), and human melanoma (Me45). The efficiency of macromolecules transport was examined with cytofluorymetry by assessing the degree of propidium iodide (PI) penetration. Additionally, cellular ultrastructure after EP was evaluated. We determined cyto-and photo-cytotoxic effect on the cells viability (MTT assay) after standard PDR and PDR combined with EP. Intracellular distribution and mitochondrial colocalization of both porphyrins was also performed. The experiments proved that both complexes exhibit desirable photodynamic properties on LoVo LoVoDX cells, and EP effectively supports photodynamic method in this type of cancer. The application of EP provided shorter time of incubation (only 10 min) and enhanced effect of applied therapy. The porphyrins did not affect the MCF-7 and Me45 cell lines.

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Section: Discussionmentioning

confidence: 96%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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“…Photosensitizer under the influence of light may induce many photochemical reactions in cells which lead to apoptosis, necrosis and autophagy [6–8]. There are many modifications of PDT which may increase its efficiency, for instance, electroporation, microdermabrasion or encapsulation into liposomes [9–12]. All above-mentioned modifications increase accumulation of photosensitizer in tissues.…”

Section: Introductionmentioning

confidence: 99%

The Use of Fractal Dimension Analysis in Estimation of Blood Vessels Shape in Transplantable Mammary Adenocarcinoma in Wistar Rats after Photodynamic Therapy Combined with Cysteine Protease Inhibitors

Jurczyszyn

Osiecka

Ziółkowski

2012

Computational and Mathematical Methods in Medicine

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Fractal dimension analysis (FDA) is modern mathematical method widely used to describing of complex and chaotic shapes when classic methods fail. The main aim of this study was evaluating the influence of photodynamic therapy (PDT) with cystein proteases inhibitors (CPI) on the number and morphology of blood vessels inside tumor and on increase of effectiveness of combined therapy in contrast to PDT and CPI used separately. Animals were divided into four groups: control, treated using only PDT, treated using only CPI and treated using combined therapy, PDT and CPI. Results showed that time of animal survival and depth of necrosis inside tumor were significantly higher in CPI+PDT group in contrast to other groups. The higher value of fractal dimension (FD) was observed in control group, while the lowest value was found in the group which was treated by cystein protease inhibitors. The differences between FD were observed in CPI group and PDT+CPI group in comparison to control group. Our results revealed that fractal dimension analysis is a very useful tool in estimating differences between irregular shapes like blood vessels in PDT treated tumors. Thus, the implementation of FDA algorithms could be useful method in evaluating the efficacy of PDT.

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“…It was found that Photofrin is a ligand for the mitochondrial peripheral benzodiazepine receptor, which is responsible for triggering pore transition [28]. Similar to these results, Saczko et al [29] reported mitochondrial localization of Photofrin in a human malignant melanoma cell line. Our investigation showed mitochondrial localization of Photofrin in the amelanotic cell line and the presence of Photofrin in endoplasmic reticulum in both melanoma cell lines after a short duration of incubation (4 h).…”

Section: Discussionmentioning

confidence: 53%

“…The EP supports nonselective transport of nonpermeant molecules into the cell, which can be used for gene transfection or drug delivery [27][28][29]. Electrically supported transport of drugs into cells is known as ECT [4,18,25,27,30].…”

Section: Introductionmentioning

confidence: 99%

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

Choromańska¹,

Kulbacka²,

Rembiałkowska³

et al. 2015

Melanoma Research

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Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.

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The effects of the electro-photodynamic in vitro treatment on human lung adenocarcinoma cells

Cited by 24 publications

References 24 publications

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

The Use of Fractal Dimension Analysis in Estimation of Blood Vessels Shape in Transplantable Mammary Adenocarcinoma in Wistar Rats after Photodynamic Therapy Combined with Cysteine Protease Inhibitors

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

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