The Effects of the Ethanol Extract of Dracocephalum Moldavica (Badrashbu) Against Strains of Antibiotic-Resistant Escherichia coli and Klebsiella pneumonia

Abstract

Background: Today, due to the increased drug resistance, plants are used for treatment. The aim of this study was to investigate the effect of ethanol extract of Badrashbu (Dracocephalum moldavica) against strains of antibiotic-resistant Escherichia coli and Klebsiella pneumonia. Methods: Badrashbu plant extracts were prepared using a rotary device. Escherichia coli and Klebsiella pneumonia were isolated from nosocomial infection patients at hospitals of Zabol. The MIC and MBC of Badrashbu extracts in different concentrations of bacteria were determined by disk diffusion method. Results:The results of plant extracts showed that the highest MIC (minimum inhibitory concentration) for E. coli isolates was 20 milligrams per milliliter, and 6 strains were inhibited at this concentration. The highest MIC for K. pneumonia was 40 milligrams per milliliter, and only one strain was inhibited at the concentration. This result can be used to treat the disease. Conclusions:The results of this study showed that Badrashbu extract inhibits Escherichia coli and Klebsiella pneumonia; so it can be used to reduce the plant disease.

The optimization growth of Dracocephalum forrestii in RITA® bioreactor, and preliminary screening of the biological activity of the polyphenol rich extract

“…MIC values against various pathogenic microorganisms revealed for hydromethanolic extract from D. forrestii was ranged obtained for extracts from D. kotschyi and D. moldavica which demonstrated minimal inhibitory concentration values between 1.5-20 mg/L [Kamali et al 2015, Khadije et al 2017]. Similar, antibacterial potential was observed for D. polyachetum against various S. aureus strains (MIC values of 0.78-25 mg/mL) [Yaghoobi et al 2018].…”

Evaluation of the Cytotoxic, Antioxidative and Antimicrobial Effects of Dracocephalum moldavica L. Cultivars

“…Relatively little of the available information refers to ethanolic extracts. An ethanolic extract obtained from D. moldavica cultivated in Iran was reported for its in vitro ability to inhibit the antibiotic-resistant Escherichia coli and Klebsiella pneumoniae nosocomial strains [ 21 ]. The ethyl acetate fraction of D. moldavica L. ethanolic extract demonstrated efficacy against S. aureus isolates with an established minimal inhibitory concentration (MIC) of 50% and 90% for 780 μg/mL and 1065 μg/mL, respectively, and an ability to disrupt biofilm formation from cell membrane damage.…”

“…Among flavonoids, tilianin is an interesting glycoside with important biological properties that was firstly isolated from this species [ 15 , 16 , 17 ]. Both classes of compounds are correlated to the biological activities of the species, such as its antioxidant [ 1 , 6 , 11 , 12 , 13 , 18 , 19 , 20 ], antimicrobial [ 1 , 18 , 21 , 22 ], and cardioprotective [ 15 , 23 , 24 , 25 , 26 , 27 ] effects, which are the most studied ones, together with its anti-inflammatory [ 28 , 29 ], neuroprotective [ 16 , 30 ], sedative [ 31 ], cytotoxic [ 14 , 32 ], and antidepressant [ 33 ] effects, which are lesser known. The antimicrobial potential of the species was attributed to its essential oils, linked to its antioxidant capacity and demonstrated against Staphylococcus aureus , Escherichia coli , Salmonella Typhimurium , and Listeria monocytogenes [ 1 , 18 ].…”

“…The antimicrobial potential of the species was attributed to its essential oils, linked to its antioxidant capacity and demonstrated against Staphylococcus aureus , Escherichia coli , Salmonella Typhimurium , and Listeria monocytogenes [ 1 , 18 ]. A hydroalcoholic extract obtained from the aerial parts of the species produced significant results against even antibiotic-resistant strains of E. coli and Klebsiella pneumoniae [ 21 ] and Staphylococcus aureus [ 22 ]. On the other hand, the cytotoxic activity of the species is less documented in previously performed studies, being attributed to flavonoids and studied on multiple myeloma and acute myeloid leukemia cell lines [ 14 ], but also to triterpenes and tested against cervical, lung, and hepatic cancerous cell lines [ 32 ].…”