The effect of flooding on soil enzyme activities and soil organic carbon (SOC) dynamics remains a widely debated topic. Here, we investigated spatial variations in C-hydrolyzing enzyme activities, soil C contents in different fractions [i.e. labile and recalcitrant carbon (LC and RC)] from 6 sites with four different elevations at two soil depths (0-10 cm and 10-30 cm) in riparian zones of the Three Gorges Reservoir, China. At region scales, the SOC, RC contents, and RC/SOC (RIC) generally showed decreasing tendency from the upstream to the downstream. The C-hydrolyzing enzyme activities were higher in the midstream compared to other sites, which did not correspond well with the changing trend of SOC content, but matched with the spatial variation in LC content. At ecosystem scales, the RC and RIC declined with decreased elevations, but the LC showed opposite trend. Whereas, the four C-hydrolyzing enzyme activities and the specific enzyme activities were corresponded well with the changing trend of Lc content. Soil c contents and enzyme activities were generally higher in top soil than deep soil across sites and elevation zones. These results reveal that the LC is the tightest factor in regulating C-hydrolyzing enzyme activities, whereas the soil C quality (i.e. RIC) and flooding collectively drive C-hydrolyzing enzyme activities possibly by affecting decomposition rates of SOC in the riparian zones. Anthropogenic activities such as large hydroelectric project can directly affect ecosystem processes and function by altering vegetation types, soil properties and hydrology 1,2 , and ultimately modify soil carbon (C) and nitrogen (N) cycling 3-5. Soil organic carbon (SOC), one of the largest and most susceptible C pools of the earth surface ecosystem, can act either as source or sink for C primarily counting on multiple factors including human activities 6-10. It is well known that the SOC pool is generally determined by the balance between litter C input and decomposition 7,11 , while the SOC decomposition process is predominantly catalyzed by a variety of extracellular enzymes 12-14. Particularly, these enzymes can hydrolyze substrates and catalyze important transformations involving in soil organic matter (SOM) synthesis 15,16. Although some studies have evaluated changes in soil C and N dynamics under large hydroelectric project 3,4,17 , how spatial variations in SOC dynamics link to C-hydrolyzing enzyme activities at region and/or ecosystem scales under large hydroelectric project remains largely unclear. The linkages between SOC and C-hydrolyzing enzyme activities can be influenced primarily by altering vegetation types under large hydroelectric projects 2,3,18,19. Most SOC exclusively originates from plant residues on site, and hence alterations in vegetation type are expected to change the quality and quantity of SOC, which in turn can affect C-hydrolyzing enzyme activities 20-23. For instance, previous studies have reported that soil hydrolytic enzyme activities are tightly related to SOC content 12,23,24 ,...