The effects of the surface charge and hydrophobicity of Escherichia coli on its adhesion to beef muscle

Li, J; McLandsborough, Lynne

doi:10.1016/s0168-1605(99)00159-2

Cited by 159 publications

(117 citation statements)

References 24 publications

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“…The poorly-adhered beef strain increased 10-fold when calcium chloride levels were similarly increased. This is contradictory to the results of [25], who reported that increasing ionic strength of the monovalent sodium-based phosphate buffer from 1.5 to 150 mM decreased the number of E. coli cells strongly adhering to beef muscle by about 10-fold. Other researchers found ionic strength had little impact on adhesion of Salmonella to poultry tissue [33].…”

Section: Discussioncontrasting

confidence: 99%

“…Research has found that increased cells in the initial suspension resulted in more cells adhering to beef muscle [25]. Although we were comparing adhesion to kaolinite, similar results were found for only the goose strain, but not the beef strain.…”

Section: Discussionsupporting

confidence: 77%

“…Researchers evaluated E. coli adhesion to beef muscle tissue over a wider pH range (4 to 10) and found marked differences between two strains [25]. They concluded that the influence of pH was likely due to changes in the beef muscle substratum.…”

Section: Discussionmentioning

confidence: 99%

“…Hydrophobicities of the individual E. coli strains were measured using the method of [25]. An aqueous solution of the individual E. coli strain grown in 3.5 mM CaCl 2 incubation solution was partitioned between the aqueous incubation solution and hexadecane.…”

Section: H Cell Surface Propertiesmentioning

confidence: 99%

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Effects of Strain Type and Water Quality on Soil-Associated Escherichia coli

Gallagher¹,

Lago²,

Hagedorn³

et al. 2013

IJESD

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Abstract-Escherichia coli strains from gulls, chickens, humans, Canada geese, horses, deer, and swine exhibited nearly 25-fold differences in adhesion to kaolinite particles. Hydrophobicity and zeta potential were not correlated with adhesion. There were significant differences in adhesion patterns between avian strains and most mammalian strains, while there were no differences in adhesion patterns between domestic animal strains and wild strains, or between ruminant and non-ruminant mammals. Selected strains exhibited varying responses to changes in pH, sorbent type, ionic strength, and generational cell age. The results indicate that adhesion by different strains under varying environmental conditions is more variable than previously recognized and that sediment-adhered bacteria can represent a significant population. Such wide variation in adhesion behavior could affect the assessment of bacterial contamination in receiving waters, and has implications for field sampling techniques, laboratory culture conditions, and experimental design of water quality projects, including TMDL protocols.Index Terms-E. coli, fecal coliforms, water pollution, bacterial adhesion, microbial source tracking.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: H Cell Surface Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Strain Type and Water Quality on Soil-Associated Escherichia coli

Gallagher¹,

Lago²,

Hagedorn³

et al. 2013

IJESD

View full text Add to dashboard Cite

show abstract

“…The absence of electrostatic attractive interactions between the E. coli cell walls and the hydrogels might also contribute to the lower degree of attachment. In addition, as the net charge of the cell walls of E. coli at physiological pH is negative (Li and McLandsborough 1999), a repulsive electrostatic interaction between E.coli and the anionic polySPMA can also explain the lower degree of attachment of E.coli on the anionic polySPMA. The relatively high bacterial attachment found for the polyDMAEMA-coated PP spacer probably results from attractive electrostatic interactions.…”

Section: Bacterial Attachment Testmentioning

confidence: 99%

Hydrogel-coated feed spacers in two-phase flow cleaning in spiral wound membrane elements: A novel platform for eco-friendly biofouling mitigation

et al. 2015

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Biofouling is still a major challenge in the application of nanofiltration and reverse osmosis membranes. Here we present a platform approach for environmentally friendly biofouling control using a combination of a hydrogel-coated feed spacer and two-phase flow cleaning.Neutral (polyHEMA-co-PEG10MA), cationic (polyDMAEMA) and anionic (polySPMA) hydrogels have been successfully grafted onto polypropylene (PP) feed spacers via plasmamediated UV-polymerization. These coatings maintained their chemical stability after 7 days incubation in neutral (pH 7), acidic (pH 5) and basic (pH 9) environments. Anti-biofouling properties of these coatings were evaluated by E. coli attachment assay and nanofiltration experiments at a TMP of 600 kPag using tap water with additional nutrients as feed and by using optical coherence tomography. Especially the anionic polySPMA-coated PP feed spacer shows reduced attachment of E. coli and biofouling in the spacer-filled narrow channels resulting in delayed biofilm growth. Employing this highly hydrophilic coating during removal of biofouling by two-phase flow cleaning also showed enhanced cleaning efficiency, feed channel pressure drop and flux recoveries. The strong hydrophilic nature and the presence of negative charge on polySPMA are most probably responsible for the improved antifouling behavior. A combination of polySPMA-coated PP feed spacers and two-phase flow cleaning therefore is promising and an environmentally friendly approach to control biofouling in NF/RO systems employing spiral-wound membrane modules.3

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Evaluating the role of sediment‐bacteria interactions on Escherichia coli concentrations at beaches in southern Lake Michigan

et al. 2013

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[1] Association of bacteria with suspended sediment in the water column complicates the assessment and prediction of nearshore water quality. We examine the impact of sedimentbacteria interactions on Escherichia coli (EC) levels at beaches in southern Lake Michigan using three-dimensional EC fate and transport models with and without explicit descriptions of sediment-bacteria interactions. We simulate hydrodynamics using a nested-grid numerical model and use a semiparametric wave model to compute wave heights and net bottom shear stress. The attachment-detachment dynamics of bacteria in the water column are modeled using a linear partition coefficient. The numerical models were tested against data, collected in summer 2008, which included measurements of EC at three beaches and multiple ADCP deployments for currents and waves. Our results indicate that the model that accounts for sediment-bacteria interactions describes the observed data significantly better and that sediment, directly and indirectly, interacts with bacteria to influence their fate and transport. The improvement results from the model's ability to describe the multiple short-duration, low-intensity resuspension events at our sampling sites. A major resuspension event was noted during the simulation period but the sampling frequency during the event was inadequate to resolve the details of the peak. Using the linear isotherm model to simulate attachment-detachment dynamics of bacteria in the water column, we found that the fraction of bacteria attached to suspended sediment particles in the water column is highly variable in the vertical at offshore locations but nearly constant closer to the shore.

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The effects of the surface charge and hydrophobicity of Escherichia coli on its adhesion to beef muscle

Cited by 159 publications

References 24 publications

Effects of Strain Type and Water Quality on Soil-Associated Escherichia coli

Effects of Strain Type and Water Quality on Soil-Associated Escherichia coli

Hydrogel-coated feed spacers in two-phase flow cleaning in spiral wound membrane elements: A novel platform for eco-friendly biofouling mitigation

Evaluating the role of sediment‐bacteria interactions on Escherichia coli concentrations at beaches in southern Lake Michigan

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