The effects of various mixing solutions on the biocompatibility of mineral trioxide aggregate

Abstract

NaOCl was highly cytotoxic for hOAs whilst all other mixing solutions can be considered as convenient biocompatible mixing solutions as alternatives to H2 O for clinical use.

Evaluation of the bioactivity of fluoride‐enriched mineral trioxide aggregate on osteoblasts

“…Mineral trioxide aggregate is considered the gold-standard therapeutic agent for hard tissue induction (Karygianni et al 2016, Lv et al 2017. The results of the present study reveal that the enrichment of ProRoot MTA with fluoride improves its biocompatibility in terms of osteoblast proliferation, osteogenic gene expression and extracellular matrix mineralization, whilst the cement texture modifies neither the cell adhesion nor morphology.…”

“…Currently, research interest focuses on modifying the MTA composition with the aim of improving the physical (Lee et al 2011), antimicrobial (Schmalz & Galler 2017) or handling properties (Kogan et al 2006). However, little attention has been paid so far to the biocompatibility testing of MTA supplied with additives (Karygianni et al 2016), which furthermore have not been selected according to their cell-or tissue-supporting capabilities. Such additives would ideally (i) foster bone and/or pulp cell proliferation and differentiation, and hence increase hard tissue formation, whilst simultaneously (ii) being nontoxic and legally approved as a drug, and (iii) exerting antimicrobial actions.…”

Biocompatibility and Surface Characteristics of Resin-Modified Glass Ionomer Cements with Ammonium Quaternary Compounds or Silver Nanoparticles: AnIn VitroStudy

“…In part, this is explained because all materials used in the study, both glass ionomers and antimicrobials, have a reported degree of toxicity [48,49]. Different in vitro studies assessed the cytotoxicity of GICs and RMGICs on cultured cells Journal of Nanomaterials [42][43][44][45][46][47][48][49][50][51][52]. The studies have supported the concept that leachable components of the dental materials are responsible for the cytotoxicity in cell culture.…”

“…The in vitro studies can be conducted under controlled conditions and provide a significant amount of information [38]. Different cell lines are used to probe the biocompatibility of dental materials like pulp cells extracted from the maxillary incisors of Sprague Dawley rats, multipotent stromal cells, human foreskin fibroblast, human lung fibroblast, primary human osteoblast, L929 mouse fibroblast, SV40 large T-antigen-transfected bovine dental pulpderived cells, MDPC-23 odontoblast-like cell line, primary pulp fibroblast, and human alveolar osteoblast, for example [39][40][41][42][43][44][45][46]. In this study, the MA-104 epithelial cell line was used because the glass ionomer cements are in direct contact with the epithelial tissue of the oral mucosa.…”

Treatment of severe periodontic‑endodontic combined lesions with minocycline hydrochloride ointment combined with mineral trioxide aggregate

“…Certification for its clinical use was granted by the Food and Drug Administration in 1993 and it was adopted in dental therapy in following years (6). MTA has low cytotoxicity, agreeable biocompatibility and has been certified in vivo and in vitro (7,8). When MTA is used as a repair material in the apical zone, it has been observed to induce almost no inflammatory reaction, and almost all samples with MTA surfaces demonstrate new cementum formation (8).…”