The effects of Vigna unguiculata on cardiac oxidative stress and aorta estrogen receptor-β expression of ovariectomized rats

Khusniyati, Etik; Sari, Alfie Ardiana; Yueniwati, Yuyun; Noorhamdani, Noorhamdani; Nurseta, Tatit; Keman, Kusnarman

doi:10.1016/s2305-0500(14)60037-3

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Moreover, the dose of 1.25 mg/kg /day decreased the MDA level to the same level as that of normal rats. This result is also consistent with previous studies, which showed that treatment with cowpea (V. unguiculata) extract reduced MDA levels in the heart [10] and blood serum [18].…”

Section: Discussionsupporting

confidence: 93%

“…Treatment with cowpea (V. unguiculata) extract significantly increased fibroblast proliferation at all doses. K-, negative control; K+, untreated ovariectomized rats; P1, treated with 1.25 mg/kg ; P2, treated with 2.5 mg/kg ; P3, treated with 5 mg/kg Cowpea (V. unguiculata) is a source of various isoflavones such as genistein, quercetin, and daidzein, which have antioxidant and antiinflammatory properties [10]. Isoflavones have similar molecular structures as 17β estradiol, and they also have estrogenic activity.…”

Section: Discussionmentioning

confidence: 99%

“…The rats used in this research were chosen according to several criteria based on previous research [10]. Wistar rats (Rattus norvegicus) were divided into five groups: negative control, ovariectomy, and ovariectomy treated with 1.25, 2.5, and 5 mg/kg cowpea (Vigna unguiculata) extract.…”

Section: Experimental Sample Preparationmentioning

confidence: 99%

“…Fibroblast cells play an important role in maintaining vaginal elasticity by synthesizing collagen reticular and elastic fibers and carbohydrates such as a glycosaminoglycan, proteoglycan, and glycoprotein in the extracellular matrix [7,8]. High-performance liquid chromatography has shown that Vigna unguiculata has a higher concentration of genistein and daidzein than soybean [9,10]. This study studied the effects of a phytoestrogen extract of cowpea (Vigna unguiculata) on vaginal tissue of ovariectomized rats, by evaluating SOD levels, production of MDA, and fibroblast proliferation.…”

Section: Introductionmentioning

confidence: 99%

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Phytoestrogen in cowpea (Vigna unguiculata L. Walp) (Fabaceae) extract reduces vaginal oxidative stress and increases proliferation of fibroblast in ovariectomized rats

Setyarini¹,

Wiyasa²,

Ratnawati³

et al. 2021

Trop. J. Pharm Res

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Purpose: To evaluate the effects of a phytoestrogen extract of cowpea (Vigna unguiculata) on malondialdehyde (MDA) and superoxide dismutase (SOD) levels, mitogen-activated protein kinase (MAPK) activity, as well as proliferation of fibroblasts in ovariectomized rat vagina. This is with a view to identify the optimal dose of a phytoestrogen supplement for use during the menopausal period of women. Methods: Wistar rats (Rattus norvegicus) were divided into five groups: negative control, ovariectomy, and ovariectomy groups treated with 1.25, 2.5, or 5 mg/kg of cowpea extract, respectively. The three doses were used to identify the optimal dose required to reduce vaginal oxidative stress and increase fibroblast proliferation in ovariectomized rats. After treatment, the ovariectomized rat vagina was assessed for SOD and MDA levels, MAPK activity, and fibroblast proliferation. Results: Cowpea extract at a dose of 2.5 and 5 mg/kg caused significant reduction in SOD levels in the vaginal tissue of rats compared with control (p < 0.05). In contrast, cowpea extract at 1.25 mg/kg resulted in MDA levels similar to that of control rats (p < 0.05). Cowpea extract treatment had positive effects on SOD and MDA levels and on fibroblast proliferation. Increase in SOD levels in rat vagina was induced by increased MDA levels, which is characteristic of oxidative stress, while ovariectomy resulted in decreased MAPK activity. Conclusion: Overall, the cowpea extract has no significant effect on MAPK activity or fibroblast proliferation. However, it has a significant effect on SOD and MDA levels and on fibroblast proliferation at a dose of 2.5 mg/kg.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytoestrogen in cowpea (Vigna unguiculata L. Walp) (Fabaceae) extract reduces vaginal oxidative stress and increases proliferation of fibroblast in ovariectomized rats

Setyarini¹,

Wiyasa²,

Ratnawati³

et al. 2021

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…As a result, there was migration of smooth muscle cells from tunica media to sub endothelial layer and plague formation. [22][23][24] Patel states that aorta has slightly different characteristics with other blood vessels, in which it is more vulnerable to vasodilatation effect than occlusion as a result of aging. The increase of blood pressure and atherosclerosis may accelerate this process and cause aneurysm.…”

Section: 56mentioning

confidence: 99%

The effect of isoflavones on diameter change of hypo estrogenic rat abdominal aorta measured by 12- MHz ultrasound

Yueniwati

Indah

Nurseta

2016

Bangladesh J Med Sci

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Background:The decrease of estrogen level causes atherosclerosis as well as the decline of vascular relaxation ability that, in turns, causes the narrowing of blood vessel's lumen. Phytoestrogens give positive effect during the atherosclerosis process and vascular motor tone. Objectives: to find out the effect of isoflavones on the diameter change of hypo estrogenic rats abdominal aortic measured by 12-MHz ultrasound. Materials and Methods: Thirty female rats were divided into 5 groups (n = 6 each) including 1 control group (sham), one ovariectomized (OVX) group, and three test groups. The test rats were OVX group received 0.5 mg/kg BW, 2.5 mg/kg BW and 5 mg/kg BW of isoflavones. Aortic diameter was analyzed in abdominal aorta using ultrasound with 12-MHz linear probes and on histologic specimens. Results: The decreasing of abdominal aortic diameter in all groups shows no significant difference (p>0.05). The measurement using ultrasound does not show any difference compared with that of histologic specimen (p=0.418), with the sensitivity value of 64.7%, specificity of 61.5% and accuracy of 63.3%. Discussion: There is no effect on the giving of isoflavones of several doses during 4-week-period on the diameter of hypo estrogenic rat's abdominal aorta. The measurement of rat's abdominal aortic diameter using 12-MHz ultrasound is compatible with the measurement on the histologic specimens.

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Other Health Benefits of African Medicinal Spices and Vegetables

Kuete¹

2017

Medicinal Spices and Vegetables From Africa

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The effects of Vigna unguiculata on cardiac oxidative stress and aorta estrogen receptor-β expression of ovariectomized rats

Cited by 5 publications

References 38 publications

Phytoestrogen in cowpea (Vigna unguiculata L. Walp) (Fabaceae) extract reduces vaginal oxidative stress and increases proliferation of fibroblast in ovariectomized rats

Phytoestrogen in cowpea (Vigna unguiculata L. Walp) (Fabaceae) extract reduces vaginal oxidative stress and increases proliferation of fibroblast in ovariectomized rats

The effect of isoflavones on diameter change of hypo estrogenic rat abdominal aorta measured by 12- MHz ultrasound

Other Health Benefits of African Medicinal Spices and Vegetables

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