The effects of vitamin B12 on the TLR-4/NF-κB signaling pathway in ovarian ischemia-reperfusion injury-related inflammation

Deniz, Esra; Topçu, Atilla; Ozturk, Aykut; Öztürk, Seda Duman; Arpa, Medeni; Atak, Mehtap

doi:10.1016/j.intimp.2022.108676

Cited by 8 publications

(3 citation statements)

References 37 publications

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“…Our present data found that LF reduced the expressions of critical genes and proteins in the TLR4/NF-κB signaling pathway in the ovaries of POF mice. These findings were similar to those from the previous literature [ 34 – 36 ]. It may be concluded that LF could prevent CTX-induced ovarian inflammation response via a TLR4/NF-κB dependent mechanism.…”

Section: Discussionsupporting

confidence: 93%

Protective effect of Luffa cylindrica fermentation liquid on cyclophosphamide-induced premature ovarian failure in female mice by attenuating oxidative stress, inflammation and apoptosis

Feng,

Zhang,

et al. 2024

J Ovarian Res

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Premature ovarian failure (POF) is a leading cause of women’s infertility without effective treatment. The purpose of this study was to investigate the protective effects of Luffa cylindrica fermentation liquid (LF) on cyclophosphamide (CTX) -induced POF in mice and to preliminarily investigate the underlying mechanisms. Thirty-two Balb/c mice were divided into four groups randomly. One group served as the control, while the other three received CTX injections to establish POF models. A 14-day gavage of either 5 or 10 μL/g LF was administered to two LF pretreatment groups. To analyze the effects of LF, the ovarian index, follicle number, the levels of serum sex hormones, superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), inflammatory factors, and apoptosis of the ovarian cells were measured. The effects of LF pretreatment on the expression of TLR4/NF-κB and apoptosis pathways were also evaluated. We found that LF pretreatment increased the ovarian index and the number of primordial and antral follicles while decreasing those of atretic follicles. LF pretreatment also increased the serum levels of estradiol (E2) and anti-Müllerian hormone (AMH), while decreasing those of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Furthermore, LF pretreatment increased the levels of SOD and GSH in the ovaries, while decreasing those of MDA, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). LF administration reduced the amount of TUNEL+ ovarian cells and the levels of TLR4 and NF-κB P65 protein expression. In conclusion, LF has antioxidant, anti-inflammatory as well as anti-apoptotic effects against CTX-induced POF, and the inhibition of TLR4/NF-κB and apoptosis pathways may be involved in its mechanisms. Graphical Abstract

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Section: Discussionsupporting

confidence: 93%

Protective effect of Luffa cylindrica fermentation liquid on cyclophosphamide-induced premature ovarian failure in female mice by attenuating oxidative stress, inflammation and apoptosis

Feng,

Zhang,

et al. 2024

J Ovarian Res

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“…The authors found that, after IR, oxidative stress and inflammation were increased and these cellular events upregulated the expression of NF-κB in IR groups 20 . A similar study was done by Deniz et al In their ovarian IR injury model on animals, they found NF-κB expression was increased in ovarian tissues 21 . In the examination of control vagina, NF-κB expression was observed in basement membrane, cell infiltrates and in the vessels.…”

Section: Discussionsupporting

confidence: 70%

E-cadherin and NF-κB expression in the vagina after ovarian ischemia and reperfusion

Yurci,

Zaman,

Sarı

et al. 2024

Acta Cir. Bras.

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Purpose: To investigate inflammation and cell adhesion molecules in the vagina after ovarian ischemia-reperfusion (IR) injury. Methods: 20 Wistar albino female rats were divided into two groups: control, and IR groups. In IR group, blood flow was restricted for 2 hours for ovarian ischemia. Then, tissues were re-blood 2 hours for reperfusion. Vagina tissues were excised and processed for histopathological analysis. Histopathological and biochemical follow-ups were performed. Results: Both malondialdehyde and myeloperoxidase values were increased in IR group compared to control group. Glutathione content was decreased in IR group compared to control group. Epithelial degeneration, inflammation, dilatation, and nuclear factor-κB (NF-κB) expression were increased in IR group compared to control group. E-cadherin expression was significantly decreased in IR group. In the IR group, E-cadherin showed a positive reaction in adenomas, gland-like cryptic structures, cellular junctions with clustered inflammatory cells. In the IR group, NF-κB expression was increased in basement membrane, inflammatory cells, in blood vessels. Conclusions: Ovarian ischemia caused degeneration of epithelial cells in the vaginal region and disruptions in the cell junction complex, which leads to activation of E-cadherin and NF-κB signaling pathway and alterations in reproductive and embryonal development in the vaginal region.

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“…A variety of models of functional ovarian damage were shown to exhibit high levels of inflammatory factors and OS markers [41,42]. Furthermore, the use of antioxidants or dietary supplements (quercetin, ginsenoside Rg1, Vitamin B12) has been shown to significantly improve both inflammation and OS, thus enhancing ovarian function [43][44][45]. Therefore, inflammation plays an important role in ovarian aging.…”

Section: Inflammationmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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Ovarian aging refers to the process by which ovarian function declines until eventual failure. The pathogenesis of ovarian aging is complex and diverse; oxidative stress (OS) is considered to be a key factor. This review focuses on the fact that OS status accelerates the ovarian aging process by promoting apoptosis, inflammation, mitochondrial damage, telomere shortening and biomacromolecular damage. Current evidence suggests that aging, smoking, high-sugar diets, pressure, superovulation, chemotherapeutic agents and industrial pollutants can be factors that accelerate ovarian aging by exacerbating OS status. In addition, we review the role of nuclear factor E2-related factor 2 (Nrf2), Sirtuin (Sirt), mitogen-activated protein kinase (MAPK), protein kinase B (AKT), Forkhead box O (FoxO) and Klotho signaling pathways during the process of ovarian aging. We also explore the role of antioxidant therapies such as melatonin, vitamins, stem cell therapies, antioxidant monomers and Traditional Chinese Medicine (TCM), and investigate the roles of these supplements with respect to the reduction of OS and the improvement of ovarian function. This review provides a rationale for antioxidant therapy to improve ovarian aging.

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The effects of vitamin B12 on the TLR-4/NF-κB signaling pathway in ovarian ischemia-reperfusion injury-related inflammation

Cited by 8 publications

References 37 publications

Protective effect of Luffa cylindrica fermentation liquid on cyclophosphamide-induced premature ovarian failure in female mice by attenuating oxidative stress, inflammation and apoptosis

Protective effect of Luffa cylindrica fermentation liquid on cyclophosphamide-induced premature ovarian failure in female mice by attenuating oxidative stress, inflammation and apoptosis

E-cadherin and NF-κB expression in the vagina after ovarian ischemia and reperfusion

The role of oxidative stress in ovarian aging: a review

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