The Efficacy in Vitro and in Vivo of Bacteriocin Against Erwinia Amylovora : Comparison of Biological and Chemical Control of Fire Blight

Jabrane, A.; Deckers, T.; Ledoux, L; Lepoivre, Philippe; Thonart, Philippe

doi:10.17660/actahortic.1996.411.72

Cited by 6 publications

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“…(B) N-terminal sequence alignment of the 43-kDa serracin P subunit, the Fels-2 prophage of Salmonella enterica (25), the phage P2 major tail sheath protein (38), the putative major tail sheath protein of Yersinia pestis prophage (31), and the CTX prophage of Pseudomonas aeruginosa (15 tide, and many other bacterial strains for which effective agents have not been determined yet (27). We have previously reported that S. plymithicum J7 crude supernatant shows antibacterial activity against fire blight in vivo in greenhouse and field experiments (19). The efficiency of the bacteriocin was generally comparable to that of streptomycin, an antibiotic used in human and animal medicine and therefore not used for plant protection in many countries (36).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora , the Fire Blight Pathogen

Jabrane

Sabri

Compère

et al. 2002

Appl Environ Microbiol

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Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon induction with mitomycin C. A phage-tail-like bacteriocin, named serracin P, was purified from an induced culture supernatant of S. plymithicum J7. It was found to be the only compound involved in the antibacterial activity against sensitive strains. The N-terminal amino acid sequence analysis of the two major subunits (23 and 43 kDa) of serracin P revealed high homology with the Fels-2 prophage of Salmonella enterica, the coliphages P2 and 168, the CTX prophage of Pseudomonas aeruginosa, and a prophage of Yersinia pestis. This strongly suggests a common ancestry for serracin P and these bacteriophages.

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Section: Discussionmentioning

confidence: 99%

Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora , the Fire Blight Pathogen

Jabrane

Sabri

Compère

et al. 2002

Appl Environ Microbiol

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“…In this study, X. perforans T3 strain bacteriocins have been shown to directly inhibit T1 strains in the greenhouse and the field. Bacteriocins have been observed in lab settings for many phytopathogenic bacteria, including Agrobacterium (28,48), Burkholderia solanacearum (12), Corynebacterium (7, 15) Erwinia (5,7,20,21), Pseudomonas (12,14,42), Xanthomonas (1,10,17,56), and Vibrio (18); however, few bacteria have been shown to have a competitive advantage over the nonproducing strains in the field. Pseudomonas syringae strain PSW-1 was shown to express a bacteriocin that can suppress sensitive strains in bean hypocotyls at certain concentrations, and the bacteriocin activity can be isolated from infected hypocotyls of bean (42).…”

Section: Discussionmentioning

confidence: 99%

Relative Importance of Bacteriocin-Like Genes in Antagonism ofXanthomonas perforansTomato Race 3 toXanthomonas euvesicatoriaTomato Race 1 Strains

Hert

Roberts

Momol

et al. 2005

Appl Environ Microbiol

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In a previous study, tomato race 3 (T3) strains of Xanthomonas perforans became predominant in fields containing both X. euvesicatoria and X. perforans races T1 and T3, respectively. This apparent ability to take over fields led to the discovery that there are three bacteriocin-like compounds associated with T3 strains. Bacterial spot of tomatoes and peppers is caused by Xanthomonas campestris pv. vesicatoria. Pohronezny and Volin (34) estimated that as high as 50% loss of marketable fruit was due to bacterial spot on tomatoes. Control of bacterial spot of tomato is difficult when high temperatures and high moisture exist. Bactericides, such as fixed coppers and streptomycin, have provided the primary means of control (30,45,46); however, streptomycin-resistant mutants and copper-tolerant strains became prevalent on treated plants (46). Marco and Stall (30) showed that many Xanthomonas euvesicatoria strains were tolerant of copper and addition of mancozeb to copper sprays improved control of the copper-tolerant strains (6, 30). However, they also showed that this treatment is insufficient when conditions favorable for disease development exist.On tomato, three races, designated tomato race 1 (T1), T2, and T3, were identified based on their reaction on three tomato genotypes (25,26, 44). Recent reclassification of the xanthomonads associated with bacterial spot of tomato has changed the species classification of these races. Currently, T1 strains are classified as X. euvesicatoria, T2 strains are classified as X. vesicatoria, and T3 strains are now classified as Xanthomonas perforans (24). T1 and T2 were first identified in 1990 (55) and are distributed worldwide (3), although only T1 strains were present in Florida until the early 1990s. In 1991, T3 appeared in Florida fields (26) and eventually predominated (22). This new race had antagonistic activity toward T1 strains (8) and was determined to be a new group designated group C (23). Genetically, T3 strains are most closely related to T1 strains based on DNA similarity values (23).In a previous study, wild type (WT) T3 strains were shown to be antagonistic toward WT T1 strains (8). Tudor-Nelson et al. (52) identified three cosmid clones, designated BCN-A, BCN-B, and BCN-C, which were found to confer the ability to inhibit a sensitive T1 strain in plate assays. These compounds were determined to have narrow inhibition spectra and fit the definition of a bacteriocin described by Reeves (36) based on the following criteria: (i) the presence of a biologically active protein moiety, (ii) inducibility with mitomycin C, and (iii) non-self inhibition (52). The three clones isolated by TudorNelson et al. (52) were unique in activity and specificity based on differential inhibition of selected sensitive T1 and T2 strains (52). It has been speculated that the bacteriocins may confer in part or completely this competitive advantage.Of the bacteriocins which have been well characterized in

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Enzybiotics: Endolysins and Bacteriocins

et al. 2017

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The Efficacy in Vitro and in Vivo of Bacteriocin Against Erwinia Amylovora : Comparison of Biological and Chemical Control of Fire Blight

Cited by 6 publications

References 0 publications

Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora , the Fire Blight Pathogen

Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora , the Fire Blight Pathogen

Relative Importance of Bacteriocin-Like Genes in Antagonism ofXanthomonas perforansTomato Race 3 toXanthomonas euvesicatoriaTomato Race 1 Strains

Enzybiotics: Endolysins and Bacteriocins

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