The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Sun, Tao; Liu, Xuesheng; Su, Yunzhe; Wang, Zihang; Cheng, Baohua; Dong, Na; Wang, Jiajun; Shan, Anshan

doi:10.1002/btm2.10446

Cited by 10 publications

(7 citation statements)

References 69 publications

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“…Diarrhea and colitis caused by bacterial infection are common diseases in both humans and animals (Hauck et al, 2016 ; Sun et al, 2022 ; Jia et al, 2023 ). Antibiotics have been widely used to treat bacteria-induced colitis.…”

Section: Introductionmentioning

confidence: 99%

Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota

et al. 2023

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Humic acids (HAs) are natural polymers with diverse functional groups that have been documented and utilized in traditional Chinese medicine. Dextran sulfate sodium (DSS)-induced colitis has been used as a model to study inflammatory bowel disease. In this research, we investigate the effect of HAs on ameliorating DSS-induced colitis in mice. Our aim here was to investigate if HAs could be a remedy against colitis and the mechanisms involved. The results show that HAs facilitated a regain of body weight and restoration of intestinal morphology after DSS-induced colitis. HAs treatment alters the community of gut microbiota with more Lactobacillus and Bifidobacterium. Changes in bacterial community result in lower amounts of lipopolysaccharides in mouse sera, as well as lower levels of inflammatory cytokines through the Toll-like receptor 4 (TLR4)-NF-κB pathway. HAs also promoted the expression of tight junction proteins, which protect the intestinal barrier from DSS damage. Cell experiments show that HAs display an inhibitory effect on DSS growth as well. These results suggest that HAs can alleviate colitis by regulating intestinal microbiota, reducing inflammation, maintaining mucosal barriers, and inhibiting pathogen growth. Thus, HAs offer great potential for the prevention and treatment of colitis.

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Section: Introductionmentioning

confidence: 99%

Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota

et al. 2023

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“…The intestinal surface is covered by a mucus layer, including mucins, AMPs, and secretory IgA, which provides the proper distance to prevent bacterial–epithelial interactions. ,, Once the function of the epithelial barrier is impaired, leading to the development of intestinal inflammation. AMPs are typically expressed by intestinal epithelial cells and defend against invading pathogens .…”

Section: Discussionmentioning

confidence: 99%

Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway

Gao

Yang

Liu

et al. 2022

J. Agric. Food Chem.

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Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways. The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms. The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model. We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge. Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice. In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis. Meanwhile, tryptophan also reshaped the gut microbiota composition in LPSchallenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%). Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response. Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy. Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.

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“…Several small-molecule drugs have been found to affect the composition and function of the gut microbiota, which, in turn, may alter the host immune system and vaccine efficacy. For example, a small molecule bioactive peptide R7I could affect the composition of gut microbiota in mice model 108 . The R7I treatment drastically lowered the presence of Clostridia and increased the dominance of Odoribacteraceae , an efficient isoalloLCA-synthesizing strain that was able to ward off potential pathogens.…”

Section: Small-molecule Drugsmentioning

confidence: 99%

A comprehensive perspective on the interaction between gut microbiota and COVID-19 vaccines

Hong

Lan

et al. 2023

Gut Microbes

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The efficacy of COVID-19 vaccines varies between individuals and populations, and the reasons for this are still not fully understood. Recent clinical studies and animal models have indicated that the gut microbiota may influence the immunogenicity of the vaccine and, thus, its effectiveness. This suggests that there is a bidirectional relationship between the gut microbiota and the COVID-19 vaccine, with the varying components of the microbiota either enhancing or reducing the vaccine’s efficacy. To put an end to the spread of the COVID-19 pandemic, the necessity of vaccines that create powerful and long-term immunity is now more important than ever, and understanding the role of the gut microbiota in this process is essential. Conversely, COVID-19 vaccines also have a significant effect on the gut microbiota, decreasing its total number of organisms and the variety of species present. In this Review, we analyze the evidence that suggesting an interaction between the gut microbiota and COVID-19 vaccine effectiveness, consider the immunological mechanisms that may be responsible for this connection, and explore the possibility of using gut microbiota-focused interventions to improve the efficacy of COVID-19 vaccines.

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The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Cited by 10 publications

References 69 publications

Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota

Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota

Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway

A comprehensive perspective on the interaction between gut microbiota and COVID-19 vaccines

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