The Efficacy of Sodium Aescinate on Cutaneous Wound Healing in Diabetic Rats

Abstract

This study is aimed to evaluate the potential effects of sodium aescinate (SA, the sodium salt of aescin) on wound healing in streptozotocin-induced diabetic rats. An excision skin wound was created in diabetic rats, and the wounded rats were divided into three groups: I) control group, II) gel-treated group, and III) SA-treated group. The control group wounds received topically normal saline once daily for 19 days. The gel-treated and SA-treated wounds received topically 400 μl of pluronic F-127 gel (25%) and 400 μl of SA (0.3%) in pluronic gel, respectively, once daily for 19 days. SA application in diabetic rats increased the wound contraction and significantly decreased the level of the inflammatory cytokine tumor necrosis factor-alpha (TNF-α) in comparison to the gel-treated group and control group. SA application in diabetic rats also resulted in a marked increase in the level of anti-inflammatory cytokine interleukin-10 (IL-10) and activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) compared to the other groups. Histopathologically, SA-treated wounds showed better granulation tissue dominated by marked fibroblast proliferation, and wounds were covered by thick regenerated epithelial layer. Additionally, the application of only pluronic gel produced some beneficial effects in some parameters in comparison to control group, but most of them were not significantly different. These findings demonstrated that SA may effectively control and improve wound healing in diabetic rats via its anti-inflammatory and antioxidant activities.

Effect of Thymoquinone on Wound Healing in Alloxan-Induced Diabetic Rats

“…This shows that hyperglycemic metabolic impairment affects wound healing ability. Chronic wound healing in diabetic patients usually manifests badly in diabetic ulcer, and this occurs as a consequence of excess reactive oxygen species (ROS) and dysfunctional inflammatory function [21,25]. It is usually characterized by continual influx of neutrophils that release cytotoxic enzymes and inflammatory mediators that cause damage to surrounding tissue [26].…”

“…In addition, lipid peroxidation is suspected to impair VEGF expression for normal angiogenesis process [35]. Antioxidant agents are effective to combat excessive ROS activity and thus minimize lipid peroxidation [22,25]. TQ has shown to improve endothelial function by reducing oxidative stress [36].…”

Effect of sodium aescinate on methyl parathion-induced myocardial injury in rats

“…Many research studies have demonstrated that SA exerts anti-inflammatory and antioxidant effects, suggesting a mechanism for its myocardial protective effects [12]. AOPP induced many complications, including myocardial injury, partly due to oxidative damage and apoptosis of myocardial cells [5].…”

Clinical effects of joint application of β-sodium aescinate and mannitol in treating early swelling after upper limb trauma surgery

“…β-sodium aescinate is a sodium containing compound extracted from the Saul son fruit (traditional Chinese medicine), with anti-inflammatory properties (5). …”

“…β-sodium aescinate is a sodium containing compound extracted from the Saul son fruit (traditional Chinese medicine), with anti-inflammatory properties (5). It is known (6) that β-sodium aescinate has relatively good efficacy in treating ischemia-reperfusion injury after acute cerebral hemorrhage and brain edema.…”