The efficacy of using RGD peptide sequence on in vitro wound healing

Kahraman, Emine; Demirci, Emine Afra; Tanyolac, Zeynep Gulce; Yarali, Ziysan Buse; Karaman, Ozan

doi:10.1109/biyomut.2016.7849408

Cited by 2 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, this compound was selected for wound healing (9). Investigations have revealed that TRP has numerous biological and pharmacological activities, such as antimicrobial, anti-cancer, anti-inflammatory, anti-allergic, and anti-protozoal effects (8)(9)(10)(11)(12). Moreover, compounds coated with TRP (13,(19)(20)(21) have been shown to have anti-inflammatory, antimicrobial, wound healing, anti-tumor, anti-allergic, and other biological activities.…”

Section: Discussionmentioning

confidence: 99%

“…It can also be gained from other herbal species, such as Isatis, Clanthe, Wrightia, and Couroupota (9). Many studies have revealed that TRP has many biological and pharmacological activities, including antimicrobial, anti-inflammatory, anti-allergic, anti-cancer, anti-protozoal, and anti-allergic effects (8)(9)(10)(11)(12). The excisional wound (EW) model utilized in the present research is the most used wound model, and it is easy to register the time-dependent closure rate of the wound by forming an open wound (13).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Lead Target Molecule for Excisional Wound Healing: Trypthantrin Compound

Kutlu¹,

Halıcı

Gedikli

et al. 2022

Iran J Pharm Res

View full text Add to dashboard Cite

Objectives: We aimed to evaluate the impact of the tryptanthrin (TRP) compound, with antimicrobial and anti-inflammatory effects, on the excisional wound (EW) model. In an EW model in mice, we tried to explain the possible effect of TRP through vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) that contribute significantly to wound healing. Methods: A total of 90 BALB-C female mice aged 6 - 8 weeks were used in the present study. Animals were randomly divided into five groups. After creating the EW model, three different doses (1, 2.5, 5 mg/kg) of TRP compound were applied topically for 14 days, and wound closure rates were measured on days 0, 3, and 7. Vascular endothelial growth factor and MMP-9 were evaluated on days 3, 7, and 14 on wound explants and on day 14 on serum samples by enzyme-linked immunosorbent assay. Histopathological analysis was performed on wound explants. Results: After the EW model creation, significant healing of the wound areas was observed in the groups for which TRP was applied, especially on the third day. Moreover, in groups that received the third dose of TRP, the wound closure rate was 94%. It was found that the wound areas were closed due to the increase in TRP dose. In line with wound healing, VEGF and MMP-9 levels gradually rose on the third and seventh days and decreased on the 14th day. Conclusions: Tryptanthrin compound usage on the EW model increased wound healing and did not leave a scar after 14 days.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Lead Target Molecule for Excisional Wound Healing: Trypthantrin Compound

Kutlu¹,

Halıcı

Gedikli

et al. 2022

Iran J Pharm Res

View full text Add to dashboard Cite

show abstract

Self-assembled Arginine–Glycine–Aspartic Acid Mimic Peptide Hydrogels as Multifunctional Biomaterials for Wound Healing

Ahmadi,

Jha,

Yadav

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Clinical management of nonhealing ulcers requires advanced materials that can enhance wound closure rates without relying on the release of drugs or other growth factors to obviate systemic deleterious side effects. In our previous work, we synthesized an integrin-binding cell adhesive M NH2 {Fmoc-FFβAR(K)βA-NH 2 consisting of an RGD mimic, [R(K)], with an amide terminus}, M OH {Fmoc-FFβAR(K)βA-OH consisting of an RGD mimic, [R(K)], with acid terminus}, and M R (Fmoc-FFβARGDβA-NH 2 consisting of an RGD peptide, reference) with multifunctional activity. Here, we reported the synthesis, characterization, and performance of a reversed derivative, R-M NH2 (Fmoc-FFβA(K)RβA-NH 2 consisting of an RGD mimic, [K(R)], with an amide terminus) of an antimicrobial cell adhesive peptide, M NH2 . Both peptides (M NH2 and R-M NH2 ) were found to interact with αvβ3 integrin, as shown by docking studies; however, they differed in cell adhesive properties, hydrogel formation, and antimicrobial efficacy. Later, the wound healing ability of a series of RGD/RGD peptide mimics (M R , R-M NH2 , M NH2 , and M OH ) was studied in a methicillin-resistant Staphylococcus aureus (MRSA)-infected Balb/c mouse model. All studied peptides showed cell adhesion and wound healing properties; however, only the amide-terminal RGD peptide mimic, M NH2 , and its reversed derivative, R-M NH2 , showed antimicrobial activity in both in vitro and in vivo studies. Of these, M NH2 showed the highest integrin-mediated spreading, migration, and proliferation of dermal cells in vitro as well as in vivo. Therefore, the M NH2 peptide mimic represents a paradigm shift in the development of dermoconductive strategies to treat chronic wounds.

show abstract

The efficacy of using RGD peptide sequence on in vitro wound healing

Cited by 2 publications

References 3 publications

A Lead Target Molecule for Excisional Wound Healing: Trypthantrin Compound

A Lead Target Molecule for Excisional Wound Healing: Trypthantrin Compound

Self-assembled Arginine–Glycine–Aspartic Acid Mimic Peptide Hydrogels as Multifunctional Biomaterials for Wound Healing

Contact Info

Product

Resources

About