The Efficiency of<i>Schistosoma mansoni</i>Crude Antigens in Inhibition of Heat Shock Protein, Apoptosis, and Lysosomal Activity: An Immunohistochemical Study

Alnasser, Sulaiman Mohammed; Alotaibi, Meshal; Ramadan, Nancy K; Abd‐Elhafeez, Hanan H.; Abdel-Hakeem, Sara S.

doi:10.1093/micmic/ozac053

Cited by 5 publications

(1 citation statement)

References 50 publications

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“…Following the manufacturer’s instructions, anti-polyvalent horseradish peroxidase/3,3′-diaminobenzidine (DAB), a ready-to-use reagent (Thermo Fisher Scientific, Waltham, MA, USA), was used to achieve antigen localization using the avidin–biotin complex technique in the Lab Vision Ultra Vision Detection System 29 . The procedures were carried out following 30 – 34 .…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

Soliman

2024

Sci Rep

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Telocytes are a unique interstitial cell type that functions in adulthood and embryogenesis. They have characteristic immunohistochemical phenotypes while acquiring different immunohistochemical properties related to the organ microenvironment. The present study aims to investigate the immunohistochemical features of embryonic telocytes during myogenesis and describe their morphology using light microscopy and TEM. Telocytes represent a major cellular constituent in the interstitial elements. They had distinguished telopodes and podoms and formed a 3D interstitial network in the developing muscles. They formed heterocellular contact with myoblasts and nascent myotubes. Telocytes also had distinctive secretory activity. Telocytes identified by CD34. They also express CD68 and MMP-9 to facilitate the development of new tissues. Expression of CD21 by telocytes may reveal their function in immune defense. They also express VEGF, which regulates angiogenesis. In conclusion, the distribution and immunological properties of telocytes in the myogenic tissue indicate that telocytes provide biological and structural support in the development of the myogenic tissue architecture and organization.

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

Soliman

2024

Sci Rep

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Nanocurcumin: A Promising Therapeutic Candidate for Experimental Trichinellosis

Abdel-Hakeem,

Abdel-Samiee,

Youssef

et al. 2024

Microscopy and Microanalysis

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In our pursuit of an alternative drug against Trichinella spiralis, we assessed the effectiveness of nanocurcumin in alleviating pathogenesis, parasitological factors, MMP-9 levels, and its expression in the enteral and parenteral phases of infection. The nanocurcumin particles, with a spherical shape and a size of 100 ± 20 nm, were used in the study. Eighty mice were divided into four groups: the control group, the untreated infected group, the nanocurcumin-treated group, and the albendazole-treated group. The nanocurcumin-treated group exhibited a statistically significant increase in the percentage of lymphocytes, along with a reduction in neutrophils, monocytes, and eosinophils compared to the untreated, infected group. Both the nanocurcumin (87.2 and 97.3%) and the albendazole-treated groups (99.8 and 98.2%) showed a significant reduction in the mean number of intestinal worms and encysted larvae, respectively. The treated groups exhibited normal intestinal villi, suppression of the inflammatory process, and fewer instances of degenerated larvae in the diaphragm and muscle compared to the untreated, infected group. Immunohistochemistry and ELISA analyses revealed a significant downregulation of MMP-9 levels in the intestines and muscles of the treated groups. Our data demonstrate that nanocurcumin contains highly versatile molecules capable of modulating biological activity against inflammation and its pathway markers.

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Modulation of the intestinal mucosal and cell-mediated response against natural helminth infection in the African catfish Clarias gariepinus

Abdel-Hakeem,

Fadladdin,

Khormi

et al. 2024

BMC Vet Res

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Fish gut is a versatile organ serving as the primary pathway for invasion by pathogens, particularly parasites, playing a crucial role in modulating the intestinal adaptive immune response. This study aimed to investigate the cellular-mediated reaction, mucosal acidity, and the expression of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and CD68 in the intestines of catfish, Clarias gariepinus, naturally infected with helminths. Forty catfish were collected from the Nile River and examined for intestinal parasites. The intestinal tissues of the control and infected fish were fixed for histochemical and immunohistochemical studies. Two groups of helminths were found: cestodes Tetracampos ciliotheca and Polyonchobothrium clarias, and nematodes Paracamallanus cyathopharynx, with a prevalence rate of 63.63%, 18.0%, and 18.0%, respectively. Our results showed that the infected fish had a statistically significant rise in the activity of immune cells, including mast cells, eosinophil granular cells, and dendritic cells. This correlated with upregulation in the expressions of PCNA, VEGF, and CD68. Histochemical analyses demonstrated a marked increase in acidic mucus production, Sudan black B, and bromophenol mercury blue. This study enriches our understanding of the evolution of vertebrate immunity in combating intestinal parasitic infections and the host’s adaptive responses.

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The Efficiency ofSchistosoma mansoniCrude Antigens in Inhibition of Heat Shock Protein, Apoptosis, and Lysosomal Activity: An Immunohistochemical Study

Cited by 5 publications

References 50 publications

Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

Nanocurcumin: A Promising Therapeutic Candidate for Experimental Trichinellosis

Modulation of the intestinal mucosal and cell-mediated response against natural helminth infection in the African catfish Clarias gariepinus

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