The electrochemical aspects of some biochemical systems—VII. The current-generating site in metabolizing E. coli systems

Allen, M.J.

doi:10.1016/0013-4686(66)85002-8

Cited by 14 publications

(3 citation statements)

References 6 publications

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“…The electron transfer from cell to resin may be a reason for this phenomenon, because the resin surface will be negatively charged by the electron transfer and, consequently, cations will be attracted around the surface. ALLEN et al (7)(8)(9) proposed that the electron transfer from E. coli to an electrode was due primarily to the oxidation of hydrogen produced by the decomposition of formic acid by formic dehydrogenase. MATSUNAGA et al (10) proposed, using L. fermenti, that from NAD(P)H and FADH2 electrons were released, and that these co-enzymes would be reduced again by the enzymes requiring NAD …”

Section: Discussionmentioning

confidence: 99%

The electric current from Escherichia coli and the effect of resin on it.

Morisaki

1982

J. Gen. Appl. Microbiol.

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The mechanism of electric-current generation by Escherichia coli JAM 1016 and the effect of an anion-exchange resin on the amount of the current were investigated by using the electrode system developed by MATSUNAGA et al.(1). The current was increased by addition of succinic acid or glucose, and the increased current was diminished by arsenite. Rotenone and antimycin A3, inhibitors of the respiratory chain, increased the current at lower concentrations and decreased it at higher. These results indicate that the current primarily results from respiratory activity, and that the source of electrons transferred to the electrode is located along the respiratory process. The current was diminished by addition of the resin. This result suggests that electrons can be transferred from cells to resin as well as to the electrode. The transfer of electrons to the resin changed the rate of glucose uptake and enhanced the respiratory activity of the organism.Recently MATSUNAGA et al. (1) developed an electrode system for measuring microbial populations. They reported that Saccharomyces cerevisiae and Lactobacillus fermenti generated electric current when they came into contact with the electrode and that the amount of the current was proportional to the microbial population. But the mechanism of current generation was not fully understood.On the other hand, the importance of the liquid-solid interface to microorganisms has been asserted by many authors. I supposed that the interface might affect the current, and that through this effect the physiological properties of microorganisms might be changed.On this assumption I studied the following: (1) the mechanism of the current generation by E. coli; (2) the effect of an anion-exchange resin (Dowex 1) on the current; (3) the effect of the resin on the rates of glucose uptake and product formation.

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Section: Discussionmentioning

confidence: 99%

The electric current from Escherichia coli and the effect of resin on it.

Morisaki

1982

J. Gen. Appl. Microbiol.

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“…Consequently, the study of charge transfer across the bacteria-electrode interface has attracted the attention of pioneer researchers who followed this originally Utopian line of investigation. It was the case of Milton J. Allen and coworkers during the 1960s, whose creative work illustrates the effort made and provides very interesting guidelines for actual research [33][34][35][36][37][38][39].…”

Section: Interfacial Charge Transfermentioning

confidence: 99%

Approach to Microbial Fuel Cells and Their Applications

2010

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“…To determine which of the above-mentioned possible sources of electrons were available to the electrode, experiments were performed in which the microorganisms were prevented from coming in contact with the electrode (5). This was accomplished by encasing the anode in a dialysis mem-brane.…”

Section: Overall Anodic Reaction (Doubled): 2h2mentioning

confidence: 99%