The Electrochemical Detremination of Nitric Oxide in Seawater Media with Microelectrodes

Zhengbin, Zhang; Xing, Lei; Jiang, Li‐Qing; Wang, Yongchen; Ren, Chao; Cai, Wenju

doi:10.3390/s30800304

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…The results show that NO exists in the marine phytoplankton ecological system. In order to detect NO in the marine ecological system, a sensor with detection limit of 10 −10 mol/L NO was used [6] .…”

Section: Discussionmentioning

confidence: 99%

Discovery of nitric oxide in marine ecological system and the chemical characteristics of nitric oxide

Zhengbin

Xing

et al. 2006

SCI CHINA SER B

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Nitric oxide was discovered in both the lab and the alga culture pond of Daya Bay (1-300 m 3 ) before the growth of alga reached the maximum. The results included: (1) NO was detected before the growth of alga reached the maximum in the case of red tide alga and food alga, and the concentration of NO decreased rapidly after the growth maximum; (2) the curve between NO concentration and time indicated that the concentration of NO in the daytime was more than that at night, and the maximal concentration of NO appeared in the midday (1-3 pm); (3) the growth of alga reached the maximum in the alga culture pond of Daya Bay in about 8-10 d, and NO was discovered in 5-7 d; (4) the measured NO concentration was 10 −9 mol/L, 10 −9 -10 −8 mol/L, and 10 −8 mol/L for Haeterosigma akashiwo, mixed alga in Daya Bay and Chaetoceros Curvisetus individually; (5) the relation of illumination with NO production was discussed.Keywords: marine ecological system, the detection of nitric oxide, red tide alga, the chemical characteristics of nitric oxide production.

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Section: Discussionmentioning

confidence: 99%

Discovery of nitric oxide in marine ecological system and the chemical characteristics of nitric oxide

Zhengbin

Xing

et al. 2006

SCI CHINA SER B

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“…Compared with our previous research on the detection of NO in seawater using polarography and homemade Nafion/Co(salen) modified platinum electrode [21-22], amperometric detection of nitric oxide with NO microsensors has high sensitivity (4.2×10 -10 mol/L NO), stability (relative standard deviation is 6.30‰). The method is a kind of practical NO determination method in seawater.…”

Section: Resultsmentioning

confidence: 92%

Amperometric Detection of Nitric Oxide with Microsensor in the Medium of Seawater and Its Applications

Xing¹,

Zhengbin²,

Liu

et al. 2005

Sensors

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Amperometric detection of nitric oxide with ISO-NOPMC NO microsensors (WPI) is systemically studied in the six media including seawater. The linear range of the microsensor for NO was from 10 -6 to 10 -9 mol/l and the detection limit was 4.2×10 -10 mol/L(S/N=2). With this method, we provide preliminary evidence that NO production could be a general attribute of marine alga (Haeterosigma akashiwo). Experiment conducted with inhibitor of uncoupler 2,4-DNP (2,4-dinitropheno) revealed that NR (nitrate reductase) activity is responsible for NO formation.

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“…Preparation of nitric oxide and sodium nitroprusside solutions Two milliliters of Milli-Q water sealed in a 10 mL vial was bubbled with high purity nitrogen gas (99.999%, Heli Industry Gas Center, Qingdao, China) at 10 mL min −1 (6 mm diameter tube) for at least 30 min to remove oxygen in the water. The deoxygenated water was then bubbled with high purity NO gas (99.9%, Date Gas Ltd, Dalian, China) for 30 min until reaching NO saturation with the concentration of about 1.4 × 10 −3 mol L −1 (Lantoine et al 1995;Zhang et al 2003). Standards were freshly made for each experiment, and different concentrations of NO solutions were obtained by diluting the saturated solution using Milli-Q water (18.2 MW cm, Millipore Company, USA).…”

Section: Algal Cultivationmentioning

confidence: 99%

Effects of Nitric Oxide On The Growth of The Marine Microalgae And The Parameters of Carbonate Chemistry

Li¹,

Zhao²,

Liu³

et al. 2021

Preprint

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Nitric oxide (NO) is a non-traditional regulator for the growth of plant and phytoplankton. This study monitored the growth of five marine phytoplankton species, namely, Platymonas helgolandica var. tsingtaoensis, Platymonas subcordiformis, Skeletonema costatum, Gymnodinium sp., and Prorocentrum donghaiense, and examined the parameters of the carbonate system in the culture media after adding different concentrations of NO and sodium nitroprusside (SNP, NO donor) solutions. The different concentrations of exogenous NO had various roles in the growth of these microalgae. The two food algae,namely, P. helgolandica var. tsingtaoensis, P. subcordiformis, and three red tide algae, namely S. costatum, Gymnodinium sp., and P. donghaiense showed different responses to the same NO concentration. The red tide algae were more sensitive to exogenous NO than the food algae. NO with the concentration of 1.4 × 10− 6 mol L− 1 had the optimum stimulatory effect on the growth of the microalgae, the cell density increased by 9.8 ~ 38.3%. SNP solution with the concentration of 100 µmol L− 1 inhibited the growth of the two food algae, the cell density decreased by 38.8 ~ 84.3%. Meanwhile, 10 µmol L− 1 SNP solution additions to the three red tide algae declined the cell density by 95.3 ~ 99.9%. Low concentrations of SNP (0.1 µmol L− 1 for the two food algae and 0.01 µmol L− 1 for the red tide algae) promoted the growth of microalgae slightly. Different concentrations of exogenous NO could also influence parameters of the carbonate system in the culture media. NO participates in the regulation of marine microalgae photosynthesis, which may influence the parameters of carbonate system.

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The Electrochemical Detremination of Nitric Oxide in Seawater Media with Microelectrodes

Cited by 11 publications

References 23 publications

Discovery of nitric oxide in marine ecological system and the chemical characteristics of nitric oxide

Discovery of nitric oxide in marine ecological system and the chemical characteristics of nitric oxide

Amperometric Detection of Nitric Oxide with Microsensor in the Medium of Seawater and Its Applications

Effects of Nitric Oxide On The Growth of The Marine Microalgae And The Parameters of Carbonate Chemistry

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